Labshake search
Citations for GE Life Sciences :
501 - 550 of 2258 citations for Collagen 17 alpha 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... The fractions containing proteins were loaded onto the HiTrap Desalting 5 x 5 ml (Sephadex G-25 S) (GE Healthcare, Code#: 17-1408-01) with a running condition of 50 mM Tris (pH 8.0) ...
-
bioRxiv - Neuroscience 2023Quote: ... The remaining tag and protease were removed by reverse Ni-NTA purification in batch mode (Ni-Sepharose High Performance; GE Healthcare, 17-5268-02). Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare ...
-
bioRxiv - Cell Biology 2022Quote: White blood cells were isolated from leukocyte cones (NC24) supplied by the NHS blood and transplant service by centrifugation on Ficoll-Paque Premium (GE Healthcare 17-5442-03) for 60 min at 300 g ...
-
bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was diluted to 100mM NaCl and clarified by centrifugation before application to a 5mL Heparin HiTrap HP (GE Healthcare 17-0407-01) column equilibrated with Buffer B (25mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... Reduction of disulfide bonds and alkylation of cysteine residues was performed by incubating gel fragments in 10 mM 1,4-Dithio-D-threitol (GE Healthcare; cat. #17-1318-02) at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare ...
-
bioRxiv - Molecular Biology 2023Quote: ... 300 μM 5’ thiol-modified 18-nt DNA oligonucleotides—comprised of an AAA base sequence and a 15-base secondary probe binding sequence (Eurofins)—were reduced with 50 mM dithiothreitol in PBS at room temperature for 2 hours and purified using NAP5 columns (GE Healthcare 17-0853-01). Maleimide-activated antibodies were then combined with 11 equivalents of the reduced form of the thiol-modified DNA oligonucleotides in PBS and incubated at 4℃ overnight ...
-
bioRxiv - Cancer Biology 2023Quote: ... to separate blood constituents and isolation of buffy coat density gradient centrifugation (400 × g at 18°C for 30 min) of RPMI-1640 diluted blood over a Ficoll-Paque™ Plus reagent (Cat. 17-1440-02, GE Healthcare, Piscataway, NJ) was performed ...
-
bioRxiv - Immunology 2023Quote: ... spleens were removed and meshed through a 70 µm cell strainer in PBS and lymphocytes were isolated by Ficoll-Paque (GE Healthcare, 17-5442-03). Single cell suspensions were washed and stained for flow cytometric analysis ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cargo tail peptides were isolated on gravity-flow column containing Ni-NTA agarose (GE Healthcare) followed by gel-filtration chromatography on Superdex Peptide 10/300 (GE Healthcare ...
-
bioRxiv - Biochemistry 2021Quote: ... The eluent was then separated from the Cy3-peptide using a second heparin column (GE Healthcare) and polished using a MonoQ 5/50 GL (GE Healthcare) ...
-
bioRxiv - Biochemistry 2020Quote: ... 150 μl of DEPC-treated water was then added and the reaction passed through a home-poured G-25 sephadex (GE Healthcare cat. # 17-0033-01) spin column ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... the sections were collected on coverslips (22 mm x 22 mm) previously pretreated with a silanization solution (0.3% (v/v) bind-silane (GE Healthcare Life Sciences, 17-1330-01), 0.1% (v/v ...
-
bioRxiv - Cell Biology 2022Quote: ... A tenth of the lysate was taken as TCE control and the rest was added to washed Protein G-coupled Sepharose beads (Sigma-Aldrich GE Healthcare 17-0618-01) for preclearing (30 min) ...
-
bioRxiv - Biochemistry 2021Quote: ... Secreted protein was captured by two round of batch absorption (BA) with 4 mL/L of pre-equilibrated Ni Sepharose beads (GE Healthcare, Cat #17-5318-01). The bound beads were transferred to gravity flow column and sequentially washed with 30 CVs Wash Buffer (50 mM HEPES 7.5 ...
-
bioRxiv - Microbiology 2020Quote: ... the removal of SUMO protease and SUMO tag from the cleavage reaction was performed with affinity chromatography on a HisTrap HP column (GE Healthcare, Code#:17-5248-01). The SUMO protease and SUMO tag will stay in the HisTrap HP column and the cleaved native protein will be in the flow-through fractions ...
-
bioRxiv - Neuroscience 2023Quote: ... isolation was performed by diluting sample in PBS (equivalent blood volume) and transferred on top of the Ficoll layer (GE Healthcare Cat#17-5442-02). The tubes were then centrifuged at 400xg ...
-
bioRxiv - Immunology 2023Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
-
bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from fresh whole blood of healthy donors by gradient centrifugation using Ficoll-PaqueTM PLUS (GE Healthcare, Cat# 17-1440-02). For T cell activation ...
-
bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of healthy donors by gradient centrifugation using Ficoll-PaqueTM PLUS (GE Healthcare, Cat# 17-1440-02). For T cell activation ...
-
bioRxiv - Immunology 2024Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... pombe lysate were mixed and incubated at 4 °C for 30 min before the addition of glutathione-sepharose beads (GE Healthcare, Cat#17-0756-01). After 2-hour incubation ...
-
bioRxiv - Synthetic Biology 2020Quote: ... the plate was blocked with 200 μl of a 2% (w/v) ECL Advance Blocking Reagent (GE Healthcare) solution in PBS-T (PBS supplemented with 0.1% (v/v ...
-
bioRxiv - Cell Biology 2023Quote: ... detection of antibodies was detected using the Amersham ECL Western Blotting Prime Blocking and Blotting Reagents (GE Healthcare).
-
bioRxiv - Cancer Biology 2019Quote: ... 4 mg of trypsin digested peptides were separately processed on an AKTA Purifier UPLC system (GE Healthcare) fitted with an in-house column packed with 5-μm TiO2 beads (GL Sciences ...
-
bioRxiv - Immunology 2021Quote: ... or other peptide–HLA-A2 ligand was immobilized on a streptavidin-coated BIAcore SA chip (GE Healthcare) at around 1000 resonance units (RU) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare).
-
bioRxiv - Microbiology 2024Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare) and quantified with the program ImageQuant (version 8.2 ...
-
bioRxiv - Microbiology 2024Quote: ... The radioactivity incorporated into each peptide was then determined using a Typhoon FLA 9500 phosphorimager (GE Healthcare) and quantified with the program ImageQuant (version 8.2 ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
-
bioRxiv - Immunology 2022Quote: ... The cell free supernatant derived during harvest of the fermented broth was processed using Blue Sepharose™ 6 Fast Flow column (GE Healthcare, Cat# 17-0948-01) in flow-through mode followed by a hydrophobic interaction chromatography (HIC ...
-
bioRxiv - Cell Biology 2020Quote: ... were activated by 100 mM DTT for 2 hours at room temperature in the dark and then purified using NAP5 columns (GE Healthcare Life Sciences, 17-0853-02) to remove excessive DTT ...
-
bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 18000-20000 rpm at 4 °C and supernatant was allowed to bind to Glutathione resin (GS resin) (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat no. 17-5132-02) in a batch binding mode for overnight at 4 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... GST-Myc-GFP or GST-Myc-USP48 was overexpressed in HEK293T cells and purified following modified instruction of Glutathione Sepharose 4B (GE Healthcare Life Sciences, 17-0756-01) using 1% NP40 buffer ...
-
bioRxiv - Microbiology 2020Quote: ... the filters were incubated with HRP-conjugated secondary antibodies in the respective blocking agent (NA931V and NA934V, GE Healthcare) for 1-2 h at RT or overnight at 4°C ...
-
bioRxiv - Biophysics 2020Quote: ... This solution was purified by size exclusion chromatography using a superdex peptide column (10/300, GE Healthcare, Germany) in 20 mM phosphate pH 7.4 buffer containing 4M GdnCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2F2 Fab-peptide complexes were purified by size-exclusion chromatography (Superdex 200 Increase 10/300 GL, GE Healthcare), concentrated and diluted to 10 mg/mL ...
-
bioRxiv - Biochemistry 2020Quote: ... PDI (in PBS, pH 7) was submitted to size exclusion chromatography on a Superose™ 12 10/300 GL column (GE Healthcare, Cat. No. 17-5173-01) at a flow rate of 0.5 ml/min with PDI eluting between 11 and 14 ml.
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS containing 0.1% polysorbate-20 and then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... acetonitrile (ACN) and 0.1% (v/v) trifluoro acetic acid (TFA) using a Superdex Peptide PC 3.2/30 column (GE Healthcare). After evaporating to dryness and dissolving in 4% (v/v ...
-
bioRxiv - Microbiology 2022Quote: ... the sensor peptide was purified by size exclusion chromatography using the Superdex 75 10/300 GL column (GE Healthcare) and then stored at −80 °C before use.
-
bioRxiv - Cancer Biology 2022Quote: ... peptides from whole cell samples were focused on immobilized pH gradient (IPG) gel strips (GE Healthcare, Waukesha, WI, USA) on a linear pH range of 3-10 ...
-
bioRxiv - Bioengineering 2021Quote: ... Peptides were purified through a C18 prep column against an acetonitrile gradient on an AKTApure 25 FPLC (GE Healthcare) and confirmed by MALDI-TOF-MS (Bruker) ...
-
bioRxiv - Microbiology 2022Quote: ... The solution containing the peptide was applied to a HiPrep SP FF 16/10 column (GE Healthcare Life Sciences) equilibrated with 20 mM sodium phosphate buffer at pH 3.9 ...
-
bioRxiv - Plant Biology 2019Quote: ... pastoris and the peptide was purified from the culture medium using the Fast Protein Liquid Chromatography System (GE Healthcare) and Reverse Phase-High Performance Liquid Chromatography (RP-HPLC ...
-
bioRxiv - Neuroscience 2022Quote: ... the Aβ42 peptide was recombinantly expressed in BL21*(DE3) pLysS Escherichia coli and purified with DEAE-Sepharose (GE Healthcare). To get rid of large Aβ42 aggregates ...
-
bioRxiv - Plant Biology 2023Quote: ... the digested peptide filtrate was fractionated by strong cation exchange (SCX) chromatography using an AKTA Purifier system (GE Healthcare). The dried peptide mixture was reconstituted and acidified with 2 ml buffer A (10 mM KH2PO4 in 25%[v/v] of ACN ...
-
bioRxiv - Cell Biology 2023Quote: ... The antibody was affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
-
bioRxiv - Biochemistry 2023Quote: ... Crosslinked peptides were desalted and reconstituted with SEC buffer [30% (v/v) ACN in 0.1% (v/v) TFA] and fractionated using a Superdex Peptide PC 3.2/30 column (GE Healthcare). Collected fractions were analysed by liquid chromatography-coupled tandem mass spectrometry (MS/MS ...
-
bioRxiv - Molecular Biology 2023Quote: Interactions between the DMC1 protein and the synthetic BRCA2 peptides were characterized using a VP-ITC Calorimeter (GE Healthcare). The peptides tested were wild type and F2406A variant of the PhePP (F2s6 ...
-
bioRxiv - Developmental Biology 2023Quote: ... The antibodies were affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).