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Citations for GE Life Sciences :
601 - 650 of 2097 citations for Chemokine C X C Motif Ligand 3 CXCL3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
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bioRxiv - Biochemistry 2020Quote: ... lysates were incubated overnight at 4°C with 400 μl of Glutathione Sepharose 4B per l of expression culture (GE Healthcare GE17-0756-01) with gentle rotation ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified via a two-step chromatography process involving nickel-affinity chromatography via the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S200 10/300 GL column ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was performed at 37 °C for 16 h and terminated by desalting it to 50 mM ABC buffer with HiTrap Desalting column (GE Healthcare: Chicago, IL, USA). It was further purified using Ni-NTA resin and collected the flow-through to remove the side product ...
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bioRxiv - Cell Biology 2022Quote: ... the remainders of the lysates were nutated with anti-Cdc15 polyclonal antibody (VU326) (Roberts-Galbraith et al., 2009) for 1 hr at 4°C followed by incubation with protein A sepharose (GE Healthcare; 17-5280-04) for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Filtrates were then filtered gently by vacuum (<400 mbar under-pressure) over pre-combusted GF/A filters (> 5 h at 460°C; 47 mm, GE Healthcare Whatman, Buckinghamshire, UK), and finally over 0.22 µm pre-cleaned44 hydrophilic PVDF filters (<600 mbar under-pressure ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was dried for 30 min at 80° C in a drying oven before being blocked with 5% ECL advanced blocking reagent (GE Healthcare, Piscataway, NJ, USA) for one hour ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... DTX3L and Flag epitope for 1 h at 4°C before Protein G Sepharose beads (30 μL per reaction mixture; GE Healthcare #GE17-0618-01) were added for 12 h ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biophysics 2023Quote: ... Cdc6 protein was purified from the supernatant by incubating for 1 h at 4 °C with glutathione beads Fastflow (GE Healthcare #17-5132-02). The beads were washed 20 times with 5 ml Cdc6 lysis buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... to separate blood constituents and isolation of buffy coat density gradient centrifugation (400 × g at 18°C for 30 min) of RPMI-1640 diluted blood over a Ficoll-Paque™ Plus reagent (Cat. 17-1440-02, GE Healthcare, Piscataway, NJ) was performed ...
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bioRxiv - Biochemistry 2023Quote: ... ASK1 TBD was expressed at 25 °C for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were then dried at 80°C for 45 min and exposed overnight to a storage phosphor screen (GE Healthcare, BAS-IP SR 2025), which was subsequently imaged with Typhoon FLA700 gel scanner and quantified in Fiji/ImageJ.
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bioRxiv - Bioengineering 2024Quote: ... The oligomeric state and purity were determined at 25°C by size-exclusion chromatography on a Superdex 200 10/300GL column (GE Healthcare Technologies, Chicago, IL).
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed in 1 x TBST and incubated in the appropriate secondary antibodies (anti-Rabbit, GE Healthcare, NA934V; anti-mouse, GE Healthcare, NA931V) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was centrifuged at 9,400× g at 4 °C for 20 min and the supernatant was applied onto a 5 mL His-Trap TALON crude column (GE Healthcare Bioscience, Piscataway, NJ, USA) pre-equilibrated with deionized water and Talon binding buffer ...
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bioRxiv - Bioengineering 2019Quote: ... cells debris were removed by centrifugation at 16,000 × g for 20 min at 4 °C and the supernatant (supplemented with 20 mM Imidazole) was loaded on a 1 mL Ni+-HisTrapHP column (GE Healthcare #71-5027-68 AK) and washed with a buffer containing 100 mM Tris-HCl (pH 7) ...
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bioRxiv - Molecular Biology 2023Quote: ... The LacO-specific probe was end labeled with 32P-γ-ATP for 1 h at 37 °C before cleaning with illustra ProbeQuant G-50 micro column (GE Healthcare; 28-9034-08). The blot was incubated for 2 h at 42 °C in hybridization buffer (ULTRAhyb™ Ultrasensitive Hybridization Buffer [Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Eluate was then cleaved with TEV protease overnight at 4 °C and subsequently purified further by size exclusion chromatography (Superdex 200 26/600 from GE Healthcare; cat # GE28-9893-36) in TBS ...
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bioRxiv - Cell Biology 2023Quote: ... pombe lysate were mixed and incubated at 4 °C for 30 min before the addition of glutathione-sepharose beads (GE Healthcare, Cat#17-0756-01). After 2-hour incubation ...
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bioRxiv - Cell Biology 2020Quote: ... and x-ray film (Amersham, GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... and X-ray film (GE Healthcare, Sweden) according to instructions of the manufacturers ...
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bioRxiv - Cell Biology 2022Quote: ... and X-ray film (Amersham, GE Healthcare).
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bioRxiv - Developmental Biology 2019Quote: ... the membrane was incubated with an appropriate secondary antibody (anti-rabbit or anti-mouse IgG HRP-conjugated Ab (1:10,000 in 3% skim milk in TBS, GE Healthcare), mouse TrueBlot ULTRA ...
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bioRxiv - Cell Biology 2023Quote: ... or with horseradish peroxidase (HRP)-conjugated secondary antibodies followed by a 3-min incubation with a chemiluminescent substrate (GE Healthcare) for ChemiDoc imaging ...
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bioRxiv - Biochemistry 2022Quote: ... the remaining 200 µL were snap-frozen in liquid nitrogen and stored at –80 °C) were isolated through MicroSpin S-400 columns (GE Healthcare, Cat. No. 27-5140-01), and 20 µL of 10% SDS were added ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... mice were injected with 2mg/kg body weight three times during 4-week period with miR-181a mimic (GE Healthcare, C-310435-05 conjugated to cholesterol) or custom antagomiR-181a (5’-FITC-mA*mC*mUmCmAmCmCmGmAmCmAmGmCmGmUmUmGmAmA*mT*mG*mU*mU - 3’Cholesterol ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 18000-20000 rpm at 4 °C and supernatant was allowed to bind to Glutathione resin (GS resin) (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat no. 17-5132-02) in a batch binding mode for overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates were afterwards washed 3 times with PBST and 50 μL of anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (Cytiva, GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... antigen was CENP-AHCP-3(105-183)::6xHis) and detected using an HRP-conjugated secondary antibody (rabbit or mouse; GE Healthcare). For antibody production CENP-AHCP-3(105-183)::6xHis was expressed in E ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Cell Biology 2020Quote: ... and exposed to X-ray films (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... and X-ray films (GE Healthcare, Chicago, IL).
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bioRxiv - Molecular Biology 2021Quote: A commercial SPR (BIAcore X, GE Healthcare, USA) was used to study the kinetics of binding and dissociation of lectin-Fc dimers to the trimeric full-length Spike in real time ...
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bioRxiv - Biochemistry 2020Quote: ... and exposed on X-Ray film (GE Healthcare Amersham Hyperfilm ECL ...
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bioRxiv - Biochemistry 2021Quote: ... X-ray film were purchased from GE Healthcare, Sweden ...
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bioRxiv - Molecular Biology 2020Quote: ... the clarified milk was thawed and loaded at 150 cm/h and 90 cm/h onto a 22 mL (1.6 x 11 cm) and 40 mL (1.6 x 20 cm) rPA Sepharose FF (GE Healthcare, Uppsala, Sweden) Protein A affinity chromatography column equilibrated with PBS for samples with a 25 mL and 100 mL milk starting volume ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were separated on 12.5% (v/v) acrylamide gels of 25 cm x 21 cm x 1 mm using an Ettan DALTsix electrophoresis unit (GE Healthcare). The SDS-PAGE was first run at a current of 2 W/gel for 1 h and then at a constant current of 15 W/gel for 5 h ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Cell Biology 2021Quote: ... Postnuclear supernatants were incubated with 3 μg of antibodies coupled to protein A– or protein G–Sepharose beads (GE Healthcare, Solingen, Germany) for 4 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) and peptides focused for 20 kVh ...
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bioRxiv - Cancer Biology 2021Quote: ... pH 3-10 (GE Healthcare) diluted 1:50 in 5% glycerol ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was stopped with 1.7 mM Tris-HCl pH 8.0 and crosslinked TcdA1-BSA-Lewis X was purified from excess of BSA-Lewis X by SEC using a Superose 6 3.2-300 column (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... The measurements performed using Biacore X instrument (GE Healthcare). Response regulators were coupled onto carboxymethyldextran hydrogel-coated SPR Sensorchip (XanTec bioanalytics GmbH ...
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bioRxiv - Cancer Biology 2020Quote: ... and visualized with an x-ray film (GE Healthcare).