Labshake search
Citations for GE Life Sciences :
501 - 550 of 2097 citations for Chemokine C X C Motif Ligand 3 CXCL3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...
-
bioRxiv - Cell Biology 2023Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
-
bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with a linear gradient of increasing NaCl concentration from 300 mM to 1 M in 20 mM Tris-HCl ...
-
bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
-
bioRxiv - Biochemistry 2023Quote: ... SC and the D1 domain of FcμR was first immobilized on the chip: SC was biotinylated at the C-terminal and immobilized on the SA chip (GE Healthcare), whereas FcμR-D1 was immobilized on the CM5 chip to a level of resonance units similar to pIgR ...
-
bioRxiv - Microbiology 2023Quote: ... After 2.5 days the diatom cultures were filtered (1.2 µm pore size, GF/C, Whatman, GE Healthcare, Little Chalfont, United Kingdom). The cells were washed off the filter using 5 mL ice-cold methanol/ethanol/chloroform 1:3:1 (methanol ...
-
bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were filtered and then pelleted at 300 g for 5 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
-
bioRxiv - Biophysics 2024Quote: ... The peak elution fractions were pooled and concentrated to 2 mL using a Amicon Ultra 10K MWCO centrifugal filter at 4 °C and was loaded into a HiLoad Superdex 200 16/60 column (GE healthcare) equilibrated with 20 mM HEPES pH 8.0 ...
-
bioRxiv - Biophysics 2024Quote: ... The sample was centrifuged (10 min, 20000g, 4 °C) and applied to Superdex 200 10/300 gel-filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
-
bioRxiv - Biochemistry 2024Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4 °C by injecting sample onto a Superose 6 Increase 10/ 300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
-
bioRxiv - Biophysics 2024Quote: All SPR binding experiments were performed on a CM5 chip (Cytiva) at 25 °C using a Biacore T200 Instrument (GE Healthcare). The purified CNBH domains were immobilized on the CM5 chip using a standard amine coupling chemistry in the presence of 10 mM sodium acetate buffer at pH 5.5 as the immobilization buffer (buffer used to directly dissolve ligands) ...
-
bioRxiv - Immunology 2019Quote: ... Columns were prepared by coupling Y-3 and B22-249 antibodies to CNBr-activated Sepharose (GE Healthcare) (1 mg antibody/40 mg Sepharose) ...
-
bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
-
bioRxiv - Cell Biology 2020Quote: ... The clarified lysate obtained after centrifugation (30 min at 20000 × g, 4°C) was first run on a streptavidin affinity column (StrepTrap® HP, GE Healthcare) equilibrated with septin 1 buffer (50 mM Tris-HCl pH 8 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
-
bioRxiv - Biochemistry 2020Quote: ... for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences). Method for EMSA was modified from a previously published protocol (30) ...
-
bioRxiv - Microbiology 2020Quote: ... Time-resolved imaging was performed with a DeltaVision Personal DV wide field fluorescence microscope equipped with Ultimate Focus™ capabilities and an environmental chamber warmed to 37 °C (Applied Precision). Images were taken at 5 or 10 minute intervals.
-
bioRxiv - Immunology 2022Quote: ... and resuspended in 24 mL of RT 70% PercollTM (1.088 g/mL at 22 °C; GE Healthcare Life Sciences 17-0891-01). Aliquots of 8 mL cell/PercollTM suspension were overlayed with 4 mL HBSS and centrifuged at 400 xg for 30 minutes RT ...
-
bioRxiv - Genomics 2020Quote: Time-lapse imaging of stable RPE1 cells expressing 53BP1-eGFP and Lifeact-RFP was performed in a 37°C climate chamber attached to a Deltavision microscope system and the SoftWorx 4.9 software (Applied Precision/GE Healthcare) using a 40x 1.3 NA oil-immersion objective ...
-
bioRxiv - Biophysics 2020Quote: ... Cells were incubated for 3-5 days at 27 °C and transfection efficiency was monitored regularly by observing YFP expression level using a fluorescence microscope (DeltaVision, Applied Precision, LLC). At >50% transfection efficiency ...
-
bioRxiv - Biochemistry 2019Quote: ... Lysates were clarified by centrifugation (18,000×g for 60 min at 4°C) and incubated with glutathione Sepharose 4B (GE Healthcare, Uppsala, Sweden), applied to a gravity column ...
-
bioRxiv - Genetics 2020Quote: Incorporation of [α-32P]-dCTP was measured by spotting 5 μl aliquots of the reaction mixture (after the indicated time points at 37°C) on Hybond N+ membrane strips (GE Healthcare Lifesciences). The membranes were washed (3 × with 2 × SSC and 1 × with 95% EtOH ...
-
bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
-
bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
-
bioRxiv - Cell Biology 2019Quote: ... The rest was incubated for 1 h at 4°C with 20 µl Ni Sepharose High Performance beads (GE Healthcare Life Sciences) to isolate the nanobodies ...
-
bioRxiv - Cell Biology 2019Quote: ... The lysate was cleared by centrifugation at 15’000×g for 1 h at 4°C and loaded on a His GraviTrap column (GE Healthcare Life Sciences), washed with 20 mM imidazole in PBS ...
-
bioRxiv - Biophysics 2021Quote: ... The column eluate containing cyt c was further purified through a gel filtration Superdex 75 16/600 column (GE Healthcare, Chicago, IL) phosphate buffer (25 mM sodium phosphate ...
-
bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant was transferred to a 50 mL tube containing 250 µl of Glutathione Sepharose 4B beads (GE Healthcare, 17075601) washed twice beforehand in 50 mL of PBS ...
-
bioRxiv - Plant Biology 2021Quote: ... Oligomeric states of OsbZIP47 protein was determined by analytical size exclusion chromatography (SEC) performed at 4°C on a Superdex 200 increase column (GE Healthcare Inc.) pre-equilibrated with buffer (25 mM sodium phosphate (pH 7.4 ...
-
bioRxiv - Molecular Biology 2022Quote: GST-fused bacterial expression plasmid for PAX9 was created by subcloning wild type and mutant (c.336C>G) PAX9 in BamHI and SalI site of pGEX4T1 vector (GE Healthcare, USA). E ...
-
bioRxiv - Cell Biology 2020Quote: ... Size-exclusion chromatography was performed at 4 °C using a HiLoad™ Superdex 200pg 16/600 GE preparative column (GE Healthcare, USA). The column was equilibrated with 50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2022Quote: ... The combined eluants were incubated overnight at 4 °C with 5 mL of Glutathione-Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were placed in a column and washed 3 times with 50 mL of Buffer B (480 mM NaCl ...
-
bioRxiv - Microbiology 2021Quote: ... Fusion events were quantified by detecting GFP+ pixels after 18 hours incubation at 37°C 5% CO2 using Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using the ImageQuant TL 8.2 image analysis software (GE Healthcare ...
-
bioRxiv - Cell Biology 2019Quote: Experiments were performed at 25 °C with a Biacore™ X100 or T200 apparatus (Biacore™, GE healthcare Life Sciences, Uppsala, Sweden). The experiments were performed on biotin CAPture kit sensor chips (Biacore™) ...
-
bioRxiv - Cancer Biology 2020Quote: ... + RNA was first denatured by heating at 65°C for 5 minutes and then transferred to A cellulose nitrate membrane (Amersham, GE Healthcare, US) using A Bio-dot device (US Bio-RAD).The membranes were then uV-crosslinked ...
-
bioRxiv - Synthetic Biology 2020Quote: The antigenicity of BG505 SOSIP–T33_dn2A trimer and BG505 SOSIP–T33_dn2 nanoparticle was analyzed on a BIAcore 3000 instrument at 25 °C and with HBS-EP (GE healthcare Life sciences) as running buffer ...
-
bioRxiv - Microbiology 2020Quote: ... The samples were then centrifuged (21 K, 4°C, 30 min) and the supernatant applied to an AKTA Start (GE Life Sciences) at 5 mL min−1 ...
-
bioRxiv - Microbiology 2020Quote: ... Fusion events were quantified by detecting GFP+ pixels after 18 hours incubation at 37°C 5% CO2 using Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using the ImageQuant TL 8.2 image analysis software (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... The dialyzed protein was diluted in buffer C to reach 50 mM NaCl immediately before loading to a Q-sepharose HP column (5 mL, GE Healthcare, USA), previously equilibrated with 10 CV of Buffer C ...
-
bioRxiv - Cell Biology 2021Quote: Cells were grown in appropriate synthetic dropout media at 30°C to mid-log phase (OD600 of ∼0.5) and imaged using DeltaVision Elite system (GE Healthcare Life Sciences) equipped with an Olympus IX-71 inverted microscope ...
-
bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged in a Beckman 45Ti rotor at 100,000 x g for 30 min at 4 °C and the supernatant applied to a HiLoad 26/10 Q Sepharose column (GE Life Sciences) equilibrated in 90 % buffer A and 10% buffer B (buffer A ...
-
bioRxiv - Immunology 2020Quote: ... 1 ml of the collected plasma was heat-inactivated (56°C for 40 min) and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... Membranes were washed of unbound probe with a pre-warmed (42°C) 2X saline-sodium citrate (SSC) buffer and detected by phosphor imaging (Storm™ 840, GE Healthcare).
-
bioRxiv - Microbiology 2022Quote: ... were collected by gentle vacuum filtration onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were then collected by gentle vacuum filtration of 1-2 L onto glass microfiber filters (grade GF/A, 47 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 2.0 mL centrifuge tubes (SafeLock ...
-
bioRxiv - Molecular Biology 2022Quote: ... The fusion protein was cleaved with 10% (w/w) TEV protease overnight at 4°C and the mixture was loaded on a 5 ml HisTrap™FF column (GE Healthcare). 6xHis-tag-labeled disulfide bond isomerase and TEV protease were retained ...