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Citations for GE Life Sciences :
251 - 300 of 2256 citations for AMPK alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The recombinant proteins were purified by Ni2+-affinity chromatography using Histrap HP FPLC columns (GE Healthcare) and a size-exclusion chromatography using a HiLoad 16/60 200 PG column (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was purified by TALON Superflow (GE Healthcare Life Sciences, Pittsburgh, PA, USA) according to the manufacturer’s description ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were purified from the filtrated supernatants using a HisTrap excel column (GE Healthcare). The purified proteins were biotinylated in vitro using the BirA enzyme in the presence of 0.5 mM Biotin and 10 mM ATP ...
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bioRxiv - Neuroscience 2022Quote: ... recombinant proteins were then eluted from a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was harvested and purified after 6 days with protein G sepharose beads (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with TBS + 0.1% Tween and incubated with goat anti-rabbit (1:2000, #NA934V) or anti-mouse (1:2000, #NA931V) secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Illinois) at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2020Quote: ... Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal) using an ECL kit (Amersham GE Healthcare Life Sciences) based on the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with a 1:5000 dilution of anti-rabbit (GE Healthcare, Little Chalfont, UK, NA934V) or anti-mouse secondary antibody (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated in horseradish peroxidase-labeled goat anti-rabbit or goat anti-mouse antibodies (GE Healthcare; 1:10,000) for 1 h at room temperature ...
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bioRxiv - Physiology 2019Quote: ... and subsequently with secondary anti-mouse/ -rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and revealed with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA). Blots were developed using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with anti-Rabbit HRP-conjugated secondary antibody (1:5000 of NA934, GE Healthcare) for 1 hour at room temperature and subsequently washed with PBST thrice ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated goat against mouse IgG and rabbit IgG (1:1000 for WB) were from GE Healthcare. Rabbit pAb against SYVN1 (# 13473-1-AP ...
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bioRxiv - Cell Biology 2020Quote: ... and secondary antibodies HRP-coupled anti-mouse or anti-rabbit (GE Healthcare and Jackson Laboratories, 1:5000). The cell cycle analysis was performed using HMLE cells that were fixed in 70% ethanol for 1 h on ice ...
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bioRxiv - Microbiology 2020Quote: ... while the secondary antibody (1/10,000) was a commercial Amersham ECL-HRP linked rabbit IgG (GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... These primary antibodies were detected with HRP-conjugated anti-mouse or anti-rabbit (1:5000; GE HealthCare), using the SuperSignal™ West Pico chemiluminescent substrate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were detected using either HRP-linked donkey anti–rabbit IgG (GE Healthcare, #NA934V; 1:10,000) or HRP-linked sheep anti– mouse IgG (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... Overexpressed recombinant proteins in supernatants were purified by AKTA start affinity chromatography system HiTrap Mabselect (GE Healthcare) according to the manufacturer’s instructions (S1 Fig).
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant Mtb-MenD protein was purified by IMAC with 5 mL HisTrap HP columns (GE Healthcare) using an imidazole gradient of 20–500 mM over 90 mL ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 recombinant proteins were purified over a His-TRAP FF column (GE Healthcare) with 1-mL bed volume ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... recombinant protein was purified from the filtered culture supernatant using HisTrap Excel columns (GE Healthcare Life Sciences). Each column was stored in 20% ethanol and washed with 5 column volumes (CV ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant 3CLpro were then purified using HIS Gravitrap Ni-NTA affinity columns (GE Healthcare, Chicago, IL), following the standard protocol (Kim et al. ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant WT and mutant IN molecules were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... The GST part was cleaved off from the recombinant protein using PreScission Protease (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 µl of antiserum were coupled to 60 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed three times in CPB and supplemented with 6 µl fresh rPAS ...
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bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare, Mississauga, ON). Endotoxin was removed using the Pierce™ High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant protein MdSUMO2A-HIS and MdDREB2A were purified by HIS Sepharose beads (GE Healthcare, Fairfield, CT, USA), GST-MdCE was purfied by Pierce™ Glutathione Spin Columns (16105 ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system as follows (GE Healthcare). First ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins were purified by nickel affinity chromatography using HisTrap Column (GE Healthcare Life Sciences Pittsburgh, PA). Rat polyclonal antisera against L ...
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bioRxiv - Molecular Biology 2020Quote: ... 250 μl of antiserum were coupled to 50 μl of recombinant protein A-sepharose (rPAS, GE Healthcare). Antibody beads were washed three times in PBS and treated with 5 μl fresh rPAS ...
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysisbioRxiv - Biophysics 2021Quote: ... Recombinant D9 was purified sequentially by a Ni-NTA column followed by a heparin column (GE Healthcare) and a GE Superdex 75 16/60 gel filtration column to separate aggregates and exchange into crystallization buffer (10 mM MES pH 5.6 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant CrRPE1 was injected on a Superose 6 Increase 10/300 GL column (GE Healthcare, Chicago, Illinois) and isocratically eluted in 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant protein GST-LSH10 was purified using Glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant CaM was purified using phenyl-sepharose resin (Phenyl Sepharose® 6 Fast Flow, GE Healthcare Inc.) as described (Bender et al. ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing soluble recombinant protein were pulled-down with Glutathione (GSH) Sepharose 4FastFlow (GE Healthcare, 17-5132) for 3h at 4°C with gently rocking ...
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bioRxiv - Molecular Biology 2023Quote: ... 170 µl of antiserum was coupled to 40 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed extensively in CPB and supplemented with 4 µl fresh rPAS ...
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bioRxiv - Microbiology 2024Quote: Recombinant WT and mutant IN proteins were analyzed using Superdex 200 10/300 GL column (GE Healthcare) with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... α-Rabbit-HRP (GE Healthcare), and α-Goat-HRP (GE Healthcare) ...
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bioRxiv - Plant Biology 2021Quote: ... Rabbit antisera (GE Healthcare) was used as secondary antibody with 1:5,000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice, GE Healthcare, 1:10000, Chicago, Illinois, USA). The bands were detected using chemiluminescence methods (ECLPrime® ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 1 h at room temperature with 1:10,000 dilutions of anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsburgh, PA), washed 3X with TBS-T ...
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bioRxiv - Physiology 2023Quote: ... with TBS-T and incubated (1-hour, room temperature) with HRP-linked anti-rabbit secondary antibody (1:10,000; GE Healthcare Bioscience, Baie d’Urfe, Canada). Chemiluminescence was assessed using the Luminata™ Crescendo Western HRP Substrate (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants from both approaches were filtered and mAbs were purified from culture supernatants using HiTrap protein G columns (GE Healthcare Life Sciences) according to the manufacturer’s protocol.