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Citations for GE Life Sciences :
151 - 200 of 2256 citations for AMPK alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The recombinant GST fusion proteins were affinity purified on GSH-Sepharose (GE Healthcare) from bacterial cultures incubated with 0.25 mM isopropyl-β-D-thiogalactopyranoside (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant proteins were purified with a fast flow Ni-NTA column (GE Healthcare) and eluted by 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant CoxbHtrA protein was purified using an ÄKTA avant system (GE Healthcare) equipped with a 5 mL StrepTrap HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: Recombinant RAD51 proteins were spotted on nitrocellulose membrane (GE Healthcare, Little Chalfont, UK). Membranes saturation ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). To produce antibody Fabs ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2024Quote: ... recombinant IgLON5 or LG1 protein was spotted onto a nitrocellulose membrane (GE Healthcare), membranes were blocked with 1% BSA in PBS ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... Alpha-synuclein was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction column (GE Healthcare) (76) ...
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bioRxiv - Immunology 2020Quote: ... clarified supernatants were passed over 2G12 mAb immobilized on CNBr-Activated Sepharose 4B resin (GE Healthcare; Cat# 17043001). Resin was washed with tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... and visualized using HRP-conjugated secondary antibodies (donkey anti-rabbit, 1:15,000, and sheep anti-mouse, 1:15,000, GE Healthcare) and ECL substrate (SuperSignal West Femto Maximum Sensitivity Substrate and Atto Ultimate Sensitivity Substrate ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 h at room temperature with HRP-conjugated rabbit or mouse secondary antibodies (1:50,000, GE Healthcare). Enhanced chemiluminescence (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Analysis was done by western blotting using the primary antibodies directed against H3K36me3 (ab9050, 1:2000) or H3K36me2 (ab9049, 1:2500) and as secondary antibody the anti-rabbit HRP (Na934v, GE Healthcare, 1:5000). The signal was detected by chemiluminescence after the addition of Pierce™ ECL Western Blotting substrate.
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
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bioRxiv - Biochemistry 2020Quote: ... and IgG anti-rabbit horseradish peroxidase–conjugated secondary antibody (1:10,000; GE Healthcare UK). For whole cerebellum and cerebellar slices ...
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bioRxiv - Plant Biology 2020Quote: ... we used a peroxidase-labeled anti-rabbit antibody (NIF824, GE Healthcare; 1:5000 dilution). Immunodetection was performed by incubating the membranes in the Western BLoT Quant HRP Substrate (Takara ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10000 dilution of HRP-conjugated donkey anti-rabbit antibody (GE Healthcare, NA934), and ECL detection reagent ...
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bioRxiv - Plant Biology 2020Quote: ... and HRP conjugated anti-rabbit IgG (Cat#NA934-1ML, GE Healthcare, 1:50,000 dilution). As a loading control ...
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bioRxiv - Cell Biology 2021Quote: ... followed by anti-rabbit and mouse antibodies coupled to HRP (1:2000, GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated in 1:10000 diluted anti-rabbit IgG (NA934-1ML; GE Healthcare) in TBS-T for one hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 μl of a 1:1000 dilution of anti-rabbit HRP antibody (GE Healthcare) was added per well ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibody was goat anti-rabbit IgG at 1:5000 dilution (GE Healthcare). Biotinylated proteins were blotted using HRP- conjugated Streptavidin at 1:1000 dilution (Thermo ...
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bioRxiv - Immunology 2021Quote: Kinetics binding measurement for anti-spike mAbs in this study was performed using a Biacore T200 instrument (GE Healthcare). The system was flushed with filtered 1xHBS-P+ running buffer (0.01M HEPES ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Immunology 2021Quote: ... Recombinant HA molecules were purified by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with 250 mM imidazole.
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bioRxiv - Plant Biology 2020Quote: ... The recombinant proteins were purified with a pre-packaged HisTrap FF column (GE Healthcare). The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were purified using a Ni-NTA Superflow affinity column (GE Healthcare) and Source-Q (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant proteins were purified by affinity chromatography using glutathione (GSH) agarose beads (GE Healthcare) and dialyzed in mild lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate containing recombinant proteins was passed through a PD-10 column (GE healthcare) to exchange buffer with Store Buffer (50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant protein was desalted with PD-10 column (SephadexTM G-25M, GE Healthcare), purified using a cation exchange column [HiTrap carboxymethyl (CM ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were purified by IMAC using an AKTA-pure-25 FPLC (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare) except for Fig ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare). The concentration of purified IgG was measured at OD280.
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bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated recombinant NS1 was purified by FPLC using a HisTrap HP Column (GE Healthcare), eluted with a 50–250 mM imidazole gradient ...
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bioRxiv - Bioengineering 2020Quote: ... for HRP-conjugated antibodies: donkey monoclonal anti-mouse (1:10000) and donkey monoclonal anti-rabbit antibody (1:10000, GE Healthcare) diluted in 2% BSA-TBST ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse, 1/3.000, #NA9310, GE Healthcare). An immune signal was detected using Clarity Western ECL substrate (#1705061 ...
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bioRxiv - Physiology 2019Quote: ... Equal loading of protein content was verified using alpha-tubulin as a housekeeping protein and bands visualised using ECL detection system (GE Healthcare, UK). Bands were measured using Image J densitometry ...
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bioRxiv - Immunology 2022Quote: ... HLA class-I and -II-associated peptides were isolated using the pan-HLA class I-specific mAb W6/32 and the pan-HLA class II-specific mAb TÜ39 (both in house mouse monoclonal) covalently linked to CNBr-activated Sepharose (GE Healthcare). HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Cell Biology 2020Quote: ... or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000, GE Healthcare, Buckinghamshire, UK), and SuperSigna West Femto Maximum Sensitivity Substrate (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... The following horseradish peroxidase-conjugated secondary antibodies were used: anti-rabbit (GE Healthcare; 1:3000), anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2019Quote: ... 1:3000 dilution) and then with anti-mouse or anti-rabbit secondary antibodies (GE Healthcare 1:5,000 dilution in 5% dry milk and 1X TBST) ...
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bioRxiv - Genetics 2020Quote: ... Anti-rabbit IgG or anti-mouse IgG conjugated with horseradish peroxidase (GE Healthcare, 1:3,000) and ECL (Pierce ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-Rabbit IgG Horseradish Peroxidase (HRP)-linked whole antibody (GE Healthcare, #NA934, 1:10000).
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies used were ECL-anti-rabbit HRP IgG (GE Healthcare, NA9340V, 1:10000), ECL-anti-mouse HRP IgG (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Western blots were developed with goat anti-rabbit HRP antibody (1:10,000; GE Healthcare #NA934V) and ECL reagent (GE Healthcare #RPN2106).
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bioRxiv - Immunology 2022Quote: ... followed by staining with the horseradish peroxidase (HRP)-conjugated anti-rabbit (1:50,000; GE healthcare) and anti-mouse (1:25,000 ...
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bioRxiv - Biochemistry 2020Quote: ... Horseradish peroxidase-conjugated anti-rabbit IgG (1:10,000, GE Healthcare Life Sciences, Fairfield, CT, USA) was used as the secondary antibody ...