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Citations for GE Life Sciences :
451 - 500 of 4979 citations for 7 Boc 3 chloro 5 6 7 8 tetrahydroimidazo 1 2 a pyrazine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare) with 25 mM HEPES-KOH buffer (pH 7.5).
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bioRxiv - Cell Biology 2022Quote: A full-length zebrafish calaxin sequence was subcloned into pGEX-6P-2 vector (GE Healthcare). For the mEGFP-Calaxin protein ...
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bioRxiv - Plant Biology 2022Quote: ... for PDX3 and ACTIN-2 and captured using an Amersham Imager 680 system (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was incubated for 2 h with Glutathione 4B Sepharose (GE Healthcare, Germany) followed by washing of the Sepharose with 50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 mL of Glutathione-Sepharose resin (GE Healthcare) and incubated for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 40 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Forty 2 ml fractions were collected using an ÄKTA chromatography system (GE Healthcare Life Sciences). Collected fractions were analyzed by SDS-PAGE using a 16% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP via gel filtration (Superdex 75 Increase 10/300; GE Healthcare Life Sciences). Protein peak fractions were combined and concentrated to 1 mM ...
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bioRxiv - Microbiology 2023Quote: ... RNA (2 μg) of each group was spotted on a Hybond-N+ membrane (GE Healthcare), followed by UV cross-linking (120 mJ/cm2 ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... A MonoS column (5/50 GL GE Healthcare 1 mL) was equilibrated with 2% MonoS B ...
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bioRxiv - Plant Biology 2020Quote: ... Obtained samples were fractionated on the column Superose 6 10/300 GL (50 kDa - 5 MDa range, GE Healthcare Life Sciences, Äkta FPLC) at the rate 0.5 ml/min in 20 mM Tris-HCl pH7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was injected into a Ni2+-NTA agarose affinity column (containing 5 mL Ni2+-Sepharose 6 FF beads, GE Healthcare, Cat. 17531803). The column was incubated for 15 minutes and washed twice with 30 mL washing buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... were mixed together and incubated for at least 1 h at 4 °C before removing the excess of free TcB-TcC by SEC on a Superose 6 Increase 5-150 column (GE Healthcare Life Sciences). For TcB-TcC variants containing HVR-RBD chimeras ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 times for 5 min each with 1X TBST buffer and developed by chemiluminescence (GE healthcare, RPN2106). The Odyssey FC imaging system (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Biophysics 2021Quote: ... 8 mM of NHS-Cy5 (GE Healthcare) or NHS-Sulfo-Cyanine 7 (Lumiprobe ...
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bioRxiv - Biochemistry 2019Quote: ... Superose-6 Increase (GE Healthcare) or Superdex S-200 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 1 mL IgG Sepharose beads suspension (6 Fast Flow, GE Healthcare) pre-rinsed with buffer for 20 ml lysate on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... TOFU-6/PICS-1 was further purified by HiLoad 16/600 Superdex 75 column (GE healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was applied to 1 ml Nickel resin (Ni Sepharose 6 Fast Flow, GE Healthcare) preequilibrated with 20 ml buffer A and 20 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) using a PD-10 desalting column (GE Healthcare, USA). Light chain of human enterokinase (10 μg/ml ...
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bioRxiv - Biophysics 2019Quote: ... 2% Dimethyldichlorosilane in octamethylcyclooctasilane (PlusOne Repel-Silane ES) was purchased by GE Healthcare (Chicago, IL, US). Optical adhesive Norland 65 was purchased from Norland Products ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... Total protein contents were assayed using the 2-D Quant Kit (GE Healthcare, Little Chalfont, UK), measuring OD480 in a microplate reader (Tecan Group ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysates were loaded first with 2 ml of DEAE Sepharose fast flow (GE Healthcare) and the elutes obtained by a gradient of 100-500 mM NaCl in 50 mM NaH2PO4 ...
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bioRxiv - Plant Biology 2021Quote: ... and the protein concentrations were determined using a 2-D Quant Kit (GE Healthcare; Piscataway, USA).
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bioRxiv - Molecular Biology 2021Quote: ... 500 mM NaCl and 2 mM CaCl2 (IMAC A) using a Quixstand benchtop system (GE Healthcare) with a 10 kDa molecular weight cutoff (MWCO ...
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bioRxiv - Biochemistry 2022Quote: ... the elution was concentrated to 2 mL and applied to a Superdex 75 column (GE Healthcare) for size exclusion chromatography ...
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bioRxiv - Molecular Biology 2022Quote: ... siGENOME Control Pool Non-Targeting #2 (cat# D-001206-14-20) was purchased from GE Healthcare. siRNA transfection was performed using Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... proteins in 2% SDS were bound to paramagnetic beads (SeraMag Speed beads, GE Healthcare,#45152105050250,#651521050502) by addition of ethanol to a final concentration of 50% ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM TCEP) and applied to a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), and protein purity was assessed by SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were concentrated using the 2-D Clean-Up Kit (80648451, GE Healthcare, Chicago, IL), reduced in 1 × Laemmli’s sample buffer (32.9 mM Tris HCl ...
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bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 mM EDTA/NaOH [pH 8.0]) and visualized with a Typhoon imaging system (GE Healthcare).
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 2 h with Glutathione Sepharose® 4 Fast Flow Beads (GE Healthcare) while rotating ...
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bioRxiv - Biochemistry 2024Quote: ... the soluble fraction was incubated in batch for 2 h with glutathione Sepharose 4B (GE Healthcare), previously equilibrated in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Cancer Biology 2021Quote: ... Stained plates were washed 3 times in dH2O for 5 minutes at room temperature and air dried before imaging using the Imagescanner III (GE Healthcare). Growth was determined by percentage area of the plate covered by cells using ImageJ v1.52a ...
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bioRxiv - Plant Biology 2020Quote: ... Each Z-section image is based on 15 images generated from 3 different angles and 5 different SIM patterns and reconstructed using SOFTWORX software (Applied Precision).
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bioRxiv - Cell Biology 2020Quote: ... membranes were exposed for 10-30 s (in the case of GFP-Plk4) and for 3–5 min (in the case of actin) on a Amersham Imager 680 (GE Healthcare) and images were acquired with its CCD camera.
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bioRxiv - Cell Biology 2019Quote: ... and 3’-primary amino-modified miR-430 MO (5’-TCTACCCCAACTTGATAGCACTTTC-3’) was obtained from Gene tools LLC and labeled with Cy3 NHS-ester (GE Healthcare). After the conjugation for 1 h at room temperature ...
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bioRxiv - Genetics 2021Quote: ... The membrane was washed 3 × 5 min in PBST and then visualized by Amersham ECL Prime Western Blot Detection Reagent (GE Healthcare). Images were taken by Fluorescent Image Analyzer LAS-3000 (FujiFilm ...
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bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: A total of 1.5 mg each of 0 hr and 3 or 5 hr ssDNA samples were labeled with Cy3-dUTP or Cy5-dUTP (GE Healthcare) by random priming without denaturation with 4 mg random nonamer oligo (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... For AKT1 detection, anti-AKT (pan) (11E7) (Cell Signalling Technology, #4685, 1:1000 in 5% BSA) and donkey-anti-rabbit IG (GE Healthcare, NA9340V, 1:3000 in 5% BSA) antibodies were used ...