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Citations for GE Life Sciences :
251 - 300 of 3606 citations for 6 Chloro N 5 ethoxy 1H pyrazol 3 yl 3 nitropyridin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... then washed for 3 × 20 min in 1× SSC and 0.1% SDS and imaged using a phosphorimager (GE Healthcare) and scanned using an Amersham Typhoon 5 scanner ...
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bioRxiv - Bioengineering 2020Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Cell Biology 2021Quote: ... SidK1-278-3×FLAG was further purified with a Superdex 200 10/300 Increase gel filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Samples were concentrated L using a 3 kDa Vivaspin™ 20 filter column (GE Healthcare, Mississauga, ON, μ Canada). The conformational mixtures were purified via size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2021Quote: ... 3 mM CaCl2 and 0.02% DDM) was injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), equilibrated with buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: Full-length mouse Csk (WT, H21I, K43D, and K222R3) were subcloned into a pGEX-6P-3 vector (GE Healthcare) containing an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Samples were first fully submerged in 3 mL of Ficoll-Plaque Plus endotoxin-free water (GE Healthcare, Uppsala, Sweden) in either a 6-well culture plate or 3-mL test tube for 1 hour ...
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bioRxiv - Biochemistry 2019Quote: ... was analysed at a constant temperature of 25°C under continuous flow of HBS-PE buffer (10mM HEPES pH 7.4, 3 mM EDTA, 0.005% (w/v) Surfactant P20 (GE Healthcare) at 30μl/min (sufficient to prevent mass transfer effects ...
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bioRxiv - Neuroscience 2020Quote: Resting-state fMRI data were collected on a whole-body 3 Tesla MR750 MRI scanner (GE Healthcare, Milwaukee, WI) with an 8-channel receive-only head array coil at the Laureate Institute of Brain Research ...
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bioRxiv - Evolutionary Biology 2019Quote: ... in 3 µL of water and spotted onto indicating Whatman FTA Classic Indicating cards (GE Healthcare Life Sciences, UK) using a micropipette ...
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bioRxiv - Immunology 2020Quote: ... 3-fold serial dilution (spanning a range of 1 μM to 1.4 nM) in HBS-EP buffer (GE healthcare). A high flow rate of 40 μL/min was used ...
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bioRxiv - Bioengineering 2020Quote: ... at room temperature with HBS-EP+ running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). IgGs were immobilized on CM5 chips (GE Healthcare # 29104988 ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2022Quote: ... acute- and chronic-type ATL patients with 3% dextran and the density gradient medium Ficoll-Paque Plus (GE Healthcare). For lymphoma-type ATL ...
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bioRxiv - Plant Biology 2023Quote: 3-dimensional Structured Illumination Microscopy (3D-SIM) and confocal images were acquired on a DeltaVision OMX Blaze (GE Healthcare) and LSM-Zeiss 710 respectively ...
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bioRxiv - Bioengineering 2023Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Plant Biology 2023Quote: ... Dried eluates were dissolved in 4 M urea with 0.1% ampholytes (pH 3–10; GE Healthcare, Chicago, IL, USA) and fractionated by off-gel focusing ...
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bioRxiv - Neuroscience 2023Quote: Both structural and functional MRI data were obtained using a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI). High-resolution T1-weighted structural images were captured using a three-dimensional brain-volume sequence (TE = 3.18 ms ...
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bioRxiv - Genetics 2023Quote: ... Blots were washed 3 times with 1x TBS-T and incubated with anti-mouse HRP (GE Healthcare Cat # NXA931) or anti-rabbit HRP secondary antibody (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 3 days post transfection and purified using GraviTrap TALON columns (GE Healthcare # GE29-0005-94). The columns were first equilibrated with purification buffer (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... MV samples were administered at six concentrations (1:3 dilutions in Neurobasal A (Hyclone, GE Healthcare, Chicago, IL, USA) containing Glutamax (Hyclone ...
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bioRxiv - Cell Biology 2024Quote: Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL;Mx1-Cre mice and Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL littermate controls were given 3 intraperitoneal injections of 40µg of poly I:C (GE Healthcare) dissolved in PBS over 5 days to induce Cre expression ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Microbiology 2020Quote: ... Protein sample were injected into a size exclusion column (Superose™ 6 Increase 5/150 GL, GE Healthcare Bio-Sciences) previously equilibrated with corresponding buffer and eluted directly into the SAXS flow-through capillary cell at a flow rate of 0.2 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA eluate was applied to size exclusion chromatography on a Superose 6 Increase 5/150 column (GE Healthcare) in basic buffer supplemented with 0.003 % LMNG and 0.0003 % CHS.
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
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bioRxiv - Biochemistry 2019Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM PMSF and 1x protease-inhibitor cocktail) and loaded onto a Superose™ 6 Increase column (GE Healthcare). Twenty-four fractions of 0.5 mL were collected and precipitated as described (Esteve-Bruna et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of media was concentrated over 2 ml heparin affinity medium (Heparin Sepharose 6 Fast Flow, GE Healthcare) packed into a 10 ml Poly-Prep Chromatography gravity flow column (Bio-Rad) ...
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bioRxiv - Bioengineering 2022Quote: ... the receptor fragment MET567 comprising the N-terminal SEMA domain and the cysteine-rich/PSI domain was immobilized at low-density (515 RU) using amine-coupling on a CM5 sensor chip (GE Healthcare). A blank inactivated reference channel was used to correct for non-specific binding ...
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bioRxiv - Immunology 2021Quote: ... PPACK-thrombin or fibrinogen in acetate buffer pH4.5 was immobilized to a chip surface (1000 or 10000 RU) using NHS/EDC-amine coupling and residual binding sites blocked by 1M ethanolamine (GE Healthcare). FH (0.5-62nM ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare). Approximately 1000 response units of Pf34 bait protein was captured onto the active flow cell ...
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bioRxiv - Cell Biology 2019Quote: ... as capture molecule to the dextran surface of both flow cells of a CM5 Chip (GE Heathcare) using an amine coupling kit (GE healthcare). GST tagged ligand proteins were captured on the detection flow cell of the chip and free GST was captured on the reference flow cell ...
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bioRxiv - Immunology 2023Quote: ... An Fc capture chip was produced by amine coupling of anti-mouse IgG antibody to a CM5 chip using a commercial Fc Capture Kit (GE Healthcare). First ...
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bioRxiv - Biophysics 2023Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Plant Biology 2023Quote: ... 5-mM 2-mercaptoethanol) before being applied to a HiTrap Heparin HP (GE Healthcare), and eluted by a linear NaCl gradient up to 1.0 M ...
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bioRxiv - Plant Biology 2019Quote: ... Hybond-N+ (GE Healthcare, Chicago, USA), was placed on the MS plate with 1% agar ...
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bioRxiv - Microbiology 2021Quote: ... Hybond-N membranes (Amersham/GE Healthcare) were used for Southern blots (45) ...
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bioRxiv - Biochemistry 2021Quote: ... and Amersham Hybond-N+ (GE Healthcare) membranes were equilibrated in 1X membrane wash buffer (20 mM Tris-Cl ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Cell Biology 2023Quote: ... wet electroblotting (100V for 1h) was used to transfer proteins onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...
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bioRxiv - Developmental Biology 2019Quote: ... the membrane was incubated with an appropriate secondary antibody (anti-rabbit or anti-mouse IgG HRP-conjugated Ab (1:10,000 in 3% skim milk in TBS, GE Healthcare), mouse TrueBlot ULTRA ...
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bioRxiv - Biochemistry 2019Quote: ... to 3 ml and the protein further purified by size exclusion chromatography (Hiload 16/600 Superdex 200 pg, GE healthcare) equilibrated with SEC buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... Wet gels were exposed to a phosphor screen for 0.5 h to 3 h and then scanned using a Typhoon FLA 7000 imaging system (GE Healthcare), after which gels were stained with Coomassie Brilliant Blue.