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Citations for GE Life Sciences :
51 - 100 of 3606 citations for 6 Chloro N 5 ethoxy 1H pyrazol 3 yl 3 nitropyridin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
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bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Immunology 2021Quote: ... incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... fragments or Fabs 2/1.12 or 2/6.14 were either used as analytes or amine immobilized on a CM5 chip in a Biacore 3000 (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The bioreactors were inoculated at a target cell density of 0.4 × 106 cells/mL in ActiPro batch media and fed daily beginning on Day 3 (3% (v/v) Boost 7a and 0.3% (v/v) Boost 7b (GE Healthcare)) ...
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bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
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bioRxiv - Neuroscience 2022Quote: ... and an aliquot of synaptosomal suspension (2 ml) was placed onto a 3-ml Percoll discontinuous gradient (GE Healthcare) containing (in mM ...
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bioRxiv - Immunology 2023Quote: ... [3] A HisTrap FF column (Cytiva, GE Healthcare) was used in the Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... and subcloned into pGEX-4T-3 (GE Healthcare) using BamHI and SalI ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 times for 5 min each with 1X TBST buffer and developed by chemiluminescence (GE healthcare, RPN2106). The Odyssey FC imaging system (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... and the cleaved DRH-3 CTD and DRH-3 NTD were purified using a Superdex 75 HiLoad 16/600 size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 5-15 mg ml−1 in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... peroxidase-linked species-specific F(ab’)2 fragment (from donkey) (1:10,000 in 3% skim milk/TBST, NA9340, GE Healthcare) Mouse TrueBlot ULTRA ...
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bioRxiv - Developmental Biology 2019Quote: ... peroxidase-linked species-specific F(ab’)2 fragment (from sheep) (1:10,000 in 3% skim milk/TBST, NA9310, GE Healthcare) ECL anti-rabbit IgG ...
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bioRxiv - Microbiology 2020Quote: ... The modified Cy3 labeled fragment was generated by labeling an amine-modified RNA containing a 5′ monophosphate ordered from Horizon Discovery with monoreactive Cy3 (GE Life Sciences) and the labeled RNA was purified by reverse-phase HPLC ...
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bioRxiv - Immunology 2021Quote: ... by an Amine Coupling Kit (GE Healthcare, BR-1000-50). The interaction between CyPA and CD147 was detected using the kinetic analysis/concentration series/direct binding mode ...
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bioRxiv - Microbiology 2021Quote: ... proteins were captured using an amine coupling kit (GE Healthcare), in which the carboxylmethyl dextran matrix of the sensor chip was activated by injection of a mixture of 0.2M 1-ethyl-3-[(3-dimethylamino ...
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bioRxiv - Cancer Biology 2021Quote: ... Stained plates were washed 3 times in dH2O for 5 minutes at room temperature and air dried before imaging using the Imagescanner III (GE Healthcare). Growth was determined by percentage area of the plate covered by cells using ImageJ v1.52a ...
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bioRxiv - Plant Biology 2020Quote: ... Each Z-section image is based on 15 images generated from 3 different angles and 5 different SIM patterns and reconstructed using SOFTWORX software (Applied Precision).
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bioRxiv - Cell Biology 2020Quote: ... membranes were exposed for 10-30 s (in the case of GFP-Plk4) and for 3–5 min (in the case of actin) on a Amersham Imager 680 (GE Healthcare) and images were acquired with its CCD camera.
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bioRxiv - Cell Biology 2019Quote: ... and 3’-primary amino-modified miR-430 MO (5’-TCTACCCCAACTTGATAGCACTTTC-3’) was obtained from Gene tools LLC and labeled with Cy3 NHS-ester (GE Healthcare). After the conjugation for 1 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...
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bioRxiv - Genetics 2021Quote: ... The membrane was washed 3 × 5 min in PBST and then visualized by Amersham ECL Prime Western Blot Detection Reagent (GE Healthcare). Images were taken by Fluorescent Image Analyzer LAS-3000 (FujiFilm ...
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bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: A total of 1.5 mg each of 0 hr and 3 or 5 hr ssDNA samples were labeled with Cy3-dUTP or Cy5-dUTP (GE Healthcare) by random priming without denaturation with 4 mg random nonamer oligo (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1, 5’- GATTGCTCGAATGCCCAAAG-3’) using Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to Phosphorimaging screens and analysed using a Typhoon Tryo (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: Dried blood spot (DBS) samples were collected on Whatman 3-mm filter paper (Whatmann No. 3, GE Healthcare Life Sciences, PA, USA) from enrolled patients on Day 0 (before treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... and subcloned into the pGEX-6P-3 (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... coli using the pGEX-5X-3 vector (GE Healthcare). The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... were incubated with GST or GST-tagged angulin-1 cytoplasmic region (409-575 aa or 409-570 aa) for 2-3 h at 4°C and further incubated with Glutathione Sepharose 4B beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Dynamic PET/CT imaging (2-3h) with arterial blood sampling was performed on Monkey 3 and Monkey 4 using a Discovery MI (GE Healthcare). Each animal had two baseline scans which were separated by one month for Monkey 3 and by one year for Monkey 4 ...
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bioRxiv - Biophysics 2019Quote: ... The reaction was incubated at 37°C for 2-3 hours on an orbital shaker and loaded onto an anion-exchange colum (1 ml DEAE FF, GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... The TOP3A-RMI1/2 complex was assembled by incubating purified TOP3A and RMI1/2 (1:3 ratio) followed by purification using a Superdex 200 Increase (GE Healthcare) in buffer I.
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bioRxiv - Neuroscience 2022Quote: ... The blots were visualised with the BioRad ChemiDoc XRS+ (Cohort 2) or the Amersham 6000 Gel Imager (GE Healthcare, Cohort 3), and quantified with Image Lab Software (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Neuroscience 2020Quote: The blots were subsequently washed 3 x 5 min in TBST 1X and developed with the Prime Western Blotting detection Reagent (GE Healthcare Amersham) using the LAS3000 Bioimager (Fujifilm ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Biophysics 2021Quote: ... coli cells were transformed with pGEX-4T-3 (GE Healthcare) containing the coding sequences of fascin ...
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bioRxiv - Cell Biology 2020Quote: ... we used a DeltaVision|OMX microscope version 3 (GE Healthcare) with an objective lens 100X UPlanSApo NA1.40 Oil (Olympus ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~7 mg/mL ...
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bioRxiv - Developmental Biology 2020Quote: GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).