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Citations for GE Life Sciences :
4551 - 4600 of 4960 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The reaction took place for 3 h and the radio labeled kCR probe purified in a Sephadex G25 column (Amersham, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Purified 6-His-PI3Kγ catalytic subunit17,18 was used as the soluble analyte and was injected at different concentrations over the immobilized GRK2 in SPR buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% surfactant P-20, GE Healthcare Life Sciences, Biacore Inc.). Binding experiments were performed at 25°C in SPR buffer with a flow rate of 20 μl/min ...
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bioRxiv - Biophysics 2021Quote: FH1FH2 constructs of Cdc12p (residues 882-1375) and Bni1p (residues 1227-1776) were expressed from pGEX-4T-3 plasmids (GE Healthcare) in BL21(DE3 ...
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bioRxiv - Cell Biology 2020Quote: ... N571 and N442 in frame with N-terminal tag glutathione S-transferase (GST) in pGEX-6P-3 expression plasmids (GE healthcare) were expressed in BL21-CodonPlus-RP E ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing the protein of interest were pooled and concentrated to ∼3 mg/mL using a 100kDa MWCO Vivaspin protein concentrator (GE Healthcare). The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... ELISA plates were afterwards washed 3 times with PBST and 50 μL of anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (Cytiva, GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... apposed them to a BAS-SR2040 phosphor screen (Fujifilm) for 3 days and imaged the slides using a phosphorimager (Typhoon FLA 7000; GE Healthcare). We drew ROIs onto the sections with standards using Multigauge software (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The samples were then applied to IPG strips and separated in the first dimension with the IPGphor 3 system from GE Healthcare according to their isoelectric focusing (20 C ...
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bioRxiv - Pathology 2019Quote: ... proteins were separated based on their isoelectric point on 18-cm immobilized pH gradient (IPG) gel strips of pH 3-10 (GE Healthcare). Subsequent to overnight rehydration ...
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bioRxiv - Cell Biology 2020Quote: Computerized axial tomography scanning (CT scan) was performed on anesthetized P30 mice [3% of Isoflurane (Isoba Vet)] using an eXplore ista PET-CTscan (GE Healthcare) with the following parameters ...
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bioRxiv - Cell Biology 2020Quote: ... antigen was CENP-AHCP-3(105-183)::6xHis) and detected using an HRP-conjugated secondary antibody (rabbit or mouse; GE Healthcare). For antibody production CENP-AHCP-3(105-183)::6xHis was expressed in E ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl) at approximately 3 mg/mL and then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare) equilibrated in solubilization buffer ...
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bioRxiv - Cell Biology 2020Quote: ... A probe was generated from a PCR fragment homologous to the 3’ sequence just outside of the targeted region using the AlkPhos direct labelling kit (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... injection of 185 MBq [123I]-2ß-carbometoxy-3ß-[4-iodophenyl]-N-[3-fluoropropyl]nortropane ([123I]FP-CIT, GE Healthcare, Amersham, UK). The SPECT data acquisition was begun after exactly 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... the pirA gene was cloned into XhoI and EcoRI sites of pGEX-4T-3 vector (GE Healthcare Life Sciences, Freiburg, Germany), encoding recombinant PirA with N-terminal-fused GST-tag ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was incubated for 3 h at room temperature and loaded onto a Superdex 200 10/300 GL (GE Healthcare) sizeexclusion chromatography column using buffer B as mobile phase ...
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bioRxiv - Genomics 2022Quote: ... Samples were then loaded onto a 1% agarose gel containing 3% formaldehyde and 1x MOPS and run at 75 V in 1x MOPS for ~3 hs after which RNA was transferred onto a Hybond N+ membrane (GE Healthcare) overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... then wrapped into a clear foil and exposed in the cassette with the phosphor screen for 3 days and visualized on a Phosphorimager (Typhoon FLA 7000, GE Healthcare). prDC1416 was used to generate the blot in Fig ...
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bioRxiv - Biophysics 2024Quote: ... His6-GB1-tag was subsequently cleaved by TEV protease (3 mM) and separated from bZIP on a cation exchange column (HiTrap SP; GE Healthcare) using a 0–2 M gradient of sodium chloride ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1-Cas2/3 complex was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer C ...
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bioRxiv - Microbiology 2023Quote: ... The eluted fractions were concentrated to 3 ml and applied to 120 ml HiLoad 16/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... control HUVEC or HUVEC 48 hr post-siRNA treatment were incubated with cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) overnight then resuspended in 2.2 mg/ml fibrinogen (820224 ...
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bioRxiv - Cell Biology 2024Quote: ... Parasites were synchronized to a 3-h age window by isolating late schizonts from a 60% Percoll (GE Healthcare, Life Sciences) gradient and culturing these for 3 h with fresh erythrocytes (104) ...
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bioRxiv - Neuroscience 2024Quote: All scans were conducted in a 3 Tesla General Electric scanner with an eight-channel head coil (GE MR750; GE Healthcare). Anatomical images were acquired via T1-weighted ...
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bioRxiv - Developmental Biology 2024Quote: ... a cDNA fragment encoding the C-terminal portion of mouse Ccrk (residues 289-346) was amplified by PCR and subcloned into the pGEX-4T-3 vector (GE Healthcare). The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3 ...
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bioRxiv - Microbiology 2020Quote: ... This reaction was incubated at 37°C for 1 hour and cleaned up with ProbeQuant G50 spin columns (GE Healthcare, GE28-9034-08) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... was purified using an ÄKTAprime plus fast protein liquid chromatography (FPLC) system equipped with a 1-mL His-Trap HP column (GE Healthcare-Amersham Biosciences) and the high salt buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The two fragments were fused by PCR to obtain a product corresponding to the 783 bp coding sequence of TMEM70 transcript variant 1 that was cloned into pMOSblue vector using the Blunt-Ended PCR Cloning Kit (GE Healthcare Life Sciences) to generate the pMOS-TMEM70 plasmid ...
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bioRxiv - Genetics 2021Quote: ... All digested DNA was separated by electrophoresis on a 1% TBE agarose gel and subsequently transferred by capillary transfer to an Amersham Hybond-N+ nylon membrane (GE Healthcare, Milwaukee, WI).
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bioRxiv - Neuroscience 2021Quote: ... membranes were washed with 1x TTBS and placed in a 1:25000 secondary HRP-conjugated IgG anti-rabbit or anti-mouse solution (GE Healthcare®, Sweden) according to the primary antibody datasheet specifications ...
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bioRxiv - Cell Biology 2019Quote: ... Bacterial expression used E.coli codon-optimized human Rab constructs created by cloning Rab GTPases lacking the C-terminal cysteines and with the relevant activating or inactivating point mutations (see Table 1) into the vector pGEX6p2 between EcoR1 and XhoI (GE Healthcare Life Sciences). Full-length NDE1 was PCR amplified from the plasmid HG15586 (Stratech Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Samples were incubated at room temperature for 1 h and DNA was extracted with magnetic Sera-Mag Speedbeads (GE Healthcare Life Sciences, UK) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved products were further purified by S-75 size exclusion chromatography (SEC buffer-50 mM sodium phosphate, 50 mM sodium chloride, 1 mM DTT, pH 8) (GE Healthcare Life Sciences). The purity of the protein was inspected using a 15% SDS PAGE (Figure S1) ...
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bioRxiv - Biophysics 2021Quote: ... gel filtration column with a flow rate of 1 ml/min using FPLC systems (AKTA pure 25 and AKTA FPLC, GE Healthcare Life Science). The 5 ml sample concentrated ...
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bioRxiv - Plant Biology 2021Quote: ... 100 µg of freeze-dried HMW compounds were dissolved in 1 mL of 20 mM sodium acetate buffer (pH 4.5) and eluted through a 1 ml HiTrap ANX FF column (GE Healthcare, 17-5162-01). Samples were eluted at a flow rate of 1 ml/min with 20 mM sodium acetate buffer pH 4.5 for 36 ml ...
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bioRxiv - Plant Biology 2020Quote: ... was used at a dilution of 1:5,000 and immunoreactive bands were visualized with ECL Western Blotting Detection Reagents (GE Healthcare, UK; https://www.gelifesciences.com).
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bioRxiv - Neuroscience 2019Quote: ... and 140 mM NaCl) was added and samples were pre-cleared for 1 hour using protein G sepharose (GE Healthcare, 17-0618-01). Immunoprecipitation was carried out overnight at 4 °C using anti-HA agarose beads (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were injected with mRNAs and DNAs at the four-cell stage using a PicoSpritzer setup in 1/3x MR with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing 50μg/ml Gentamycin ...
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bioRxiv - Biophysics 2022Quote: The rat Syb2 (29–93) and Syb2 (29–93, S61C), mouse Rab3A (22–217, Q81L) were all cloned into pGEX-6p-1(GE Healthcare; Piscataway, NJ). The cytoplasmic domain of rat Syx1 (2–253 ...
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bioRxiv - Developmental Biology 2021Quote: ... The bound protein was detected using rabbit anti-GH antibody at a final dilution of 1:40,000 (AFP5672099, NIDDK-NHPP) followed by horseradish peroxidase-conjugated antibody (anti-rabbit, IgG; GE Healthcare, Chicago, Illinois, USA) at a final dilution of 1:2000 ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were subsequently washed with 1 x TBS/T for 30 min in 10 min intervals prior to incubation in ECLTM (GE Healthcare, cat# RPN2106) and exposure by film (FUJIFILM Super RX ...
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bioRxiv - Molecular Biology 2021Quote: ... NET2 buffer was added to a final volume of 1 mL with 20 µL of Glutathione Sepharose 4B or SF3b155a-antibody conjugated with Protein G Sepharose (GE Healthcare Life Sciences) and incubated at 4°C for 3 h ...
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bioRxiv - Molecular Biology 2020Quote: ... one of which was heating in a 65 °C water bath for 60 minutes and the second was using a Hi Trap QHP column (1 mL GE Healthcare, United States). When loading ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed to remove unbound phages and then probed with anti-M13-HRP secondary antibody (1:5000, Cytiva [formerly GE Healthcare], Marlboro, MA) followed by TMB as noted above.
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bioRxiv - Microbiology 2019Quote: ... The bacterial protein PA1217 was fused to a GST-tag by cloning it into a pGEX-6P-1 vector (GE Healthcare, Chicago, IL) using the BamHI and EcoRI restriction sites (Thermo Scientific) ...