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Citations for GE Life Sciences :
4501 - 4550 of 4960 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL, GE Healthcare, www.gehealthcare.com) of an Äkta Avant System (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... a stabilized soluble version of the spike protein (based on GenPept:QHD43416 ORF amino acids 1–1207) was produced using an ExpiCHO Expression system (Thermoscientific, USA, Cat. No. A29133) following purification using HisTrap (GE Healthcare, U.K.) and Strep-TactinXT (IBA ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were separated by electrophoresis using a 1% agarose gel and transferred to nylon membranes (Hybond N+®, GE Healthcare), where hybridization was carried out using an 812 bp fragment of Micropia from D ...
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bioRxiv - Biochemistry 2021Quote: ... isolated E coli membranes with overexpressed MgtA were solubilized in 1% β-dodecyl maltoside (β-DDM) prior to Histrap HP (GE Healthcare) purification equilibrated in (25 mM HEPES pH 7.0 ...
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bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 14,000 rpm and proteins purified by affinity chromatography using a Ni2+ column (1 mL GE Healthcare HisTrap HP) and imidazole gradient elution in an AKTA Start protein purification system ...
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bioRxiv - Microbiology 2021Quote: ... ATP and MgCl2 were then added to a final concentration of 1 and 20 mM and then applied to a HiTrap Q column (GE Life Sciences). Separation occurred over an 18 CV gradient from 100% IEX1 to 100% IEX2 buffers [20mM Tris pH 8.0 (RT ...
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bioRxiv - Cell Biology 2022Quote: ... followed by buffer exchange into G buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA) using desalting column (GE Healthcare; cat#17085101). To remove the GST tag ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Biochemistry 2022Quote: ... The complex formed in the 1:2.5 mixture was purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in assay buffer and run at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the patients underwent a T1 weighted 1 mm isometric anatomical MRI scan using a 3T Signa HDx scanner (GE Healthcare, Chicago, Illinois). Following the implant ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl of each mucus sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), left to dry out ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 or 15 % 29:1 acryl:bis-acrylamide) and transferred on Amersham™ Protran™ 0.45 μm nitrocellulose membranes (GE Healthcare, Cat# 10600033) at 4°C for 1 hr at 110V ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM imidazole at pH 8) and loaded on a nickel affinity chromatography column (1 mL HisTrap, GE Healthcare, 17-5247-01). Bound proteins were eluted in elution buffer (20 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2024Quote: ... The tagged proteins were detected using HRP conjugated sheep anti-mouse or anti-rabbit IgG antibody (1:10000, GE Healthcare, Uppsala, Sweden) and visualized using the Amersham ECL Western blot detection reagent (GE Healthcare) ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed in PBT and incubated with HRP conjugated-anti-rabbit IgG (GE Healthcare # NA9340, 1:5000 in blocking solution) at room temperature for 1 hour and washed twice in PBT ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... CaM was eluted in buffer containing 1 mM EDTA then further purified using anion exchange with a Resource Q column (GE Life Sciences) before dialysis into water and lyophilization in a vacuum concentrator.
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were injected two times/embryo with mRNAs at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs or plasmid DNA at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid or BAC DNA was labelled by nick-translation with fluorescent dUTPs (Abott Molecular or GE HealthCare Life Science) for 3 h at 15°C as described previously41 ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-3xFLAG-V1H(1-351) was further purified by gel filtration on a Superdex 200 10/300 GL column (GE Life Sciences) into the appropriate buffer for the subsequent application ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was diluted 1:10 v/v with 20 mM Tris 8.0 and further purified with anion exchange chromatography (HiTrapQ column, GE Healthcare Life Sciences) using a NaCl gradient of 50 mM to 500 mM in 20 mM Tris 8.0 ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was washed with 0.1% TBST and incubated with corresponding secondary antibody conjugated to Horse Radish Peroxidase (HRP) at 1:10000 (GE life sciences; NA934) dilutions for one hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and detected with a 1:10000 goat anti rabbit secondary antibody conjugated with HRP and imaged with ECL (GE healthcare NA931, NA934).
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using the 2-D Quant kit (GE Life Sciences 80648356). Samples were reduced (5 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 mM NaCl and 2 mM DTT on a Superdex 200 column (GE Healthcare). In case of individual purification of PHF14 and HMG20A ...
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bioRxiv - Plant Biology 2019Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Microbiology 2019Quote: ... tuberculosis CFP was purified and concentrated using 2-D Clean-Up Kit (GE Healthcare) and resuspended in 125 µl of rehydration solution (urea 7M ...
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bioRxiv - Biochemistry 2020Quote: ... Supernates were loaded by gravity onto a Ni-2+-charged column (HisTrapHP, GE Healthcare) equilibrated with Buffer E ...
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bioRxiv - Microbiology 2020Quote: ... A 20 μL sample of Precission protease (2 U/μL; catalogue # 27084301, GE Healthcare) was added and beads were incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 2 mM 1,4-dithiothreitol) using Illustra NAP columns (GE Healthcare). Apo AfAgo sample was measured in the same buffer containing 500 mM NaCl.
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bioRxiv - Microbiology 2020Quote: ... SARS2-CoV-2 RBD-His was immobilized to a CM5 sensor chip (GE Healthcare). Serial dilutions of purified recombinant Nanosota-1 drugs were injected at different concentrations ...
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bioRxiv - Plant Biology 2021Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... spots were analyzed using Image Master 2-D Platinum image analysis software (GE Healthcare). One-way factor ANOVA (P < 0.05 ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μL of each reaction was spotted onto Protran BA85 nitrocellulose (GE Healthcare) via pipette or a replicator pinning tool (V&P Scientific ...
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bioRxiv - Immunology 2022Quote: ... for 10 min and blocked in PBS containing 2% bovine serum albumin (GE Healthcare) and 0.01% Tween 20 (National Diagnostics ...
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bioRxiv - Neuroscience 2024Quote: ... 2 μg of patient-derived AABs were dotted on a nitrocellulose membrane (GE Healthcare), membranes were probed with mouse anti-human IgG1-POD ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2 mM EDTA) and electroblotted onto a nylon (N+) membrane (GE Healthcare RPN203B) at 20 V for 90 min at 4 °C in 0.5x TBE buffer ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was incubated with 2 mL Ni-Sepharose High Performance slurry (GE Healthcare) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... for 2 h and then incubated with Streptavidin Sepharose High Performance beads (GE Healthcare) for 4 h ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Evolutionary Biology 2024Quote: ... The samples were then treated with the 2-D Clean-Up Kit (GE Healthcare) to eliminate impurities not compatible with mass spectrometry analyses and recovered in 50 mM ammonium bicarbonate buffer ...
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bioRxiv - Cell Biology 2024Quote: Cell or collagen lysates were separated by denaturing SDS-PAGE (6% gels for collagens, 10% for SMADs) and proteins blotted onto a nitrocellulose membrane (Amersham™, GE Healthcare, Chalfont St Giles, United Kingdom). Membranes were cut horizontally to detect proteins of different sizes ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Biophysics 2022Quote: ... the volume was reduced to a volume of 400 ml using the ÄktaFlux system with a 3 kDa MWCO cartridge (GE Healthcare). Afterwards the system was used for buffer exchange with 50 mM HEPES ...
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bioRxiv - Cell Biology 2019Quote: Each oligonucleotide contained an amino group at the 3’ end for fluorophore coupling using either Cy5 NHS ester (GE Healthcare, PA15102) or rhodamine NHS ester (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... Protein containing fractions were concentrated to 3 mL and further purified by size exclusion using a HiPrep 16/60 Sephacryl S-500 HR (GE Healthcare) in Buffer D (20 mM HEPES pH 7.4 ...