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Citations for GE Life Sciences :
4501 - 4550 of 4723 citations for Recombinant Human FCGR3B Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 14,000 rpm and proteins purified by affinity chromatography using a Ni2+ column (1 mL GE Healthcare HisTrap HP) and imidazole gradient elution in an AKTA Start protein purification system ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of the collected plasma was heat-inactivated (56°C for 40 min) and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Affinity-purified proteins were concentrated and further purified by size-exclusion chromatography (SEC) using a Superdex200 16/60 column (GE Life Sciences) running in 1x TBS (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... The PGT145 affinity purified SOSIP protein was further subjected to size exclusion chromatography (SEC) using a HiLoad Superdex 200 16/60 column (GE Healthcare Inc.). The SEC purified proteins were snap frozen in liquid nitrogen and stored at −80°C until further use ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were filtered through 0.1 um syringe filters and the SiaCP protein was purified using two GraviTrap™ TALON® columns (GE Healthcare). For purification ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of a last step in which the protein was dialyzed against buffer LS and then applied onto a HiTrap SP cation exchange column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... flow-through fractions from the second Ni-NTA affinity purification step containing the protein at high purity were concentrated and then applied onto a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare, USA) equilibrated with a buffer containing 50 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μg of protein per sample were solved in urea lysis buffer and labeled with 250 pmol CyDye DIGE Fluor dyes (GE Healthcare, Germany). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were resolved by SDS-PAGE and proteins were transferred to a nitrocellulose membrane (Cat#10600003, AmershamTM ProtranTM Premium 0.45 μm NC, GE Healthcare Life sciences) which were incubated in blocking buffer (5% nonfat dry milk ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed thrice with PBST and the bands corresponding to protein of interest were detected by chemiluminescence using the Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare; RPN2232) in a Fusion FX7 Spectra Multispectral Imaging system (Witec AG ...
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bioRxiv - Plant Biology 2022Quote: ... or adjusted to Chl/protein content were fractionated by electrophoresis on SDS-polyacrylamide gels (12%) and blotted onto nitrocellulose membranes (Amersham Protran, GE Healthcare, UK). Membranes were stained with Ponceau S and probed with protein-specific antibodies according to (Sambrook ...
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bioRxiv - Microbiology 2023Quote: 0.5 mg of the Ni-NTA protein eluate was analyzed by size exclusion chromatography (SEC) using a Superdex 200 10/300 column (GE Life Sciences) in 25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare, Uppsala, Sweden). Subsequently the membranes were incubated with primary antibodies and HRP-coupled secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then subjected to SDS-PAGE followed by transfer of the proteins onto nitrocellulose membranes (GE Healthcare, Chalfont St Giles, UK). Membranes were blocked in 3% BSA/PBST (1x PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then lysed in lysis buffer and equal amounts of their proteins were subjected to Streptavidin beads-based affinity isolation (GE Healthcare, USA). After 30 minutes incubation at 4 degrees ...
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bioRxiv - Biochemistry 2023Quote: The supernatant containing the target protein was then loaded on a 5 mL Ni-NTA column followed by a size exclusion column (Superdex 75, GE Healthcare, USA). YsiA is then eluted stepwise with 30 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrated protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL (GE healthcare, GE28-9909-44) in SEC buffer ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... The amount of protein present in immunoblot bands was quantified using the ImageQuant LAS 4000 system with ImageQuant TL7.0 analysis software (GE Healthcare Life Sciences) or ChemiDoc MP (Bio- Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity-purified protein was further purified (>95%) using Superdex 75 prep-grade matrix in a 16/70 C column (GE Healthcare Biosciences).
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the desired protein were pooled together and concentrated to 2ml using a 10 kDa cutoff Vivaspin20 concentrator (GE Healthcare Biosciences). Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences ...
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Improved Activity and Kinetics of Endoglucanase Biofuel Enzyme with Addition of an AzoTAB SurfactantbioRxiv - Bioengineering 2023Quote: ... The protein solution was filtered and applied to a 5 mL HiTrap Q HP anion exchange column (GE Healthcare, Cat. no. 17115301) and later washed with 30× column volume with the protein buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
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bioRxiv - Biochemistry 2023Quote: ... PD-10 desalting columns (Sephadex G-25 resin), and size exclusion chromatography column (HiLoad® Superdex, 75 pg) used for protein purification were obtained from GE Healthcare. Amorphous ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions with low protein background were pooled and subjected to size-exclusion chromatography on a Superdex 75 pg column (GE Healthcare, USA) in the storage buffer (DesAOMT ...
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bioRxiv - Microbiology 2023Quote: ... The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant; #28-9670-62, GE Healthcare) using binding buffer pH 7 ...
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bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... Peaks containing the ThsA protein were injected to a size-exclusion chromatography (SEC) column (Superdex 200_16/60, GE Healthcare, 28-9893-35) equilibrated with SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: The concentrated affinity-purified protein sample underwent further purification by fast performance liquid chromatography (FPLC) using an AKTA purifier UPC10 system (GE Life Sciences) and a Hiprep 16-60 Sephacryl S300HR size exclusion chromatography column at a flow rate of 1 ml min-1 (running buffer was the same as the binding buffer but without the imidazole) ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL of purified proteins at 5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8x-His-SUMO tag was removed by Ni-NTA agarose beads and the proteins were further purified by size exclusion chromatography using Superdex 75 16/600 (GE Life Sciences) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were visualized using, ECL prime (Amersham, Uppsala, Sweden) and detected using ImageQuant™ LAS 4000(GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were resolved on SDS-PAGE (10% acrylamide) and transferred to a nitrocellulose Hybond ECL membrane (GE Healthcare, Pittsburgh, PA, USA) for probing with high-affinity rat monoclonal antibodies anti-HA (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant LMW-SLP proteins from the soluble fraction were purified by affinity chromatography on Ni-agarose columns using an AKTA prime (GE Healthcare, #11001313). Proteins were dialyzed against 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was concentrated and subjected to gel-filtration chromatography using a Superdex 200 increase 10/300 column (GE Healthcare, USA), pre-equilibrated with gel-filtration buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: WDR5 (residues 22-334 or 1-334) and PTENα (residues 1-576) proteins were further purified using a Superdex200 gel-filtration column (GE Healthcare, 28989335) with a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... The processed proteins were purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL). Purified ...
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bioRxiv - Molecular Biology 2023Quote: ... Immuno-reactive protein bands were detected by the ECL system and visualized by imaging analyzer (ImageQuant LAS 500, GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2023Quote: The His6-tagged proteins were purified by affinity chromatography using HisTrap™ HP Ni-NTA columns and the ÄKTA purifier liquid chromatography system (GE Healthcare) according to the instructions of the manufacturers ...
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bioRxiv - Pathology 2024Quote: ... Proteins were separated by SDS-PAGE (10 % polyacrylamide gel) and transferred to a PVDF membrane (RNP303F, GE Healthcare, Little Chalfont, Buckinghamshire, UK) in a semi-dry blotter (TE70X ...
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bioRxiv - Cell Biology 2024Quote: ... the protein signal was developed by Super Signal West Femto (Thermo 34095) and visualized with Amercham Hyperfilm ECL system (GE Healthcare 28906839) film.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS– PAGE) and proteins were transferred into nitrocellulose membranes (Amersham Protran Premium 0.45μm nitrocellulose blotting membranes, GE Healthcare Life Sciences, USA). For immunostaining ...
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bioRxiv - Biochemistry 2024Quote: ... The purification of Ubi-SSFc and His6-SSFc was performed using HisTrap resin and AKTA protein purification system (Ge Healthcare Life Sciences). Firstly ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were centrifuged at 13,000 RPM for 5 minutes at 4°C and the supernatant was transferred to a new tube and cleared of residual IgG by rotation with Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The purifications of these proteins from transformed bacterial cell extract were performed according to manufacturer instructions (GST bulk kit, GE Healthcare Life Sciences). The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences) ...
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bioRxiv - Immunology 2021Quote: ... After washing the membranes with TBST, protein bands were detected with a chemiluminescent substrate (BIOMAX, USA) through a CCD Imager (GE life sciences, USA).
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bioRxiv - Molecular Biology 2020Quote: ... Elution buffer was exchanged to standard Tris protein buffer (100 mM Tris and 150 mM NaCl at pH 7.5) with NAP-5 columns (GE Healthcare, Chicago, IL, USA) and samples were concentrated as before.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified from the infected cell culture supernatants by binding to a HiTrap ProteinG HP column (GE Healthcare Life Sciences, Piscataway, NJ) and eluted with 0.1 M citrate ...
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bioRxiv - Biophysics 2022Quote: ... After purification with Ni-NTA agarose the protein was diluted to 375mM NaCl and further purified on an ÄKTA purifier system (GE Healthcare Life Sciences) at 4 °C using 1ml HiTrap™ Heparin HP column (Cytiva) ...