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Citations for GE Life Sciences :
4451 - 4500 of 4723 citations for Recombinant Human FCGR3B Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The eluted protein was then buffer exchanged to 10 mM HEPES at pH 7.4 by PD-10 columns (GE Healthcare Life Sciences) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... CALM ANTH domain and Ub proteins were further purified on a HiLoad 16/60 Superdex 200 column (GE Healthcare Biosciences, Pittsburg, PA) equilibrated with 20mM HEPES-NaOH ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed using an M-110S Microfluidizer (Microfluidics) and proteins were purified using glutathione chromatography according to the manufacturer’s instructions (GE Healthcare Life Sciences). To confirm the size and purity of purified recombinant protein ...
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bioRxiv - Plant Biology 2021Quote: ... Oligomeric states of OsbZIP47 protein was determined by analytical size exclusion chromatography (SEC) performed at 4°C on a Superdex 200 increase column (GE Healthcare Inc.) pre-equilibrated with buffer (25 mM sodium phosphate (pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: The IgG fraction from the hyperimmunized rabbit serum was purified on 2x 1 mL HiTrap™ Protein G HP columns (GE Healthcare). And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... The protein was then separated by size-exclusion chromatography (SEC) using a Superdex 75 16/600 column (GE Healthcare Life Sciences, UK) equilibrated with PBS pH 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare, UK) (Nettleship et al. ...
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bioRxiv - Biophysics 2020Quote: ... protein was concentrated and further purified by size exclusion chromatography using a HiLoad® 16/600 Superdex 200 pg column (GE Healthcare) in Dialysis Buffer ...
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bioRxiv - Biophysics 2020Quote: ... For ion exchange chromatography (IEX) the protein was loaded onto a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Sweden) and eluted against a linear gradient of 7 column volumes (CV ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 10 μg of total protein was separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane with either 0.4 μm pores (GE Healthcare, Chicago, IL) or 0.2 μm pores (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Biophysics 2020Quote: ... Unconjugated TFP ester dyes were separated from labeled proteins using HiTrap Desalting columns with Sephadex G-25 resin (GE Healthcare Life Sciences). Alexa Fluor™ 647 C2 Maleimide (A20347 ...
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bioRxiv - Molecular Biology 2020Quote: ... Flow rate was 0.75 ml/min and protein elution was monitored with the U9-L UV monitor (GE Healthcare, Chicago, IL, USA) at 280 nm.
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bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated protein was further purified using size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare, Chicago, IL, USA) column containing 0.003% (w/v ...
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bioRxiv - Immunology 2022Quote: ... anti-PD-L1 and LH02 were all purified by affinity chromatography using a protein A affinity column (GE Healthcare, Piscataway, NJ, USA) and analyzed in reducing condition on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Cell Biology 2022Quote: ... We performed immunoprecipitations with precoated anti-SRSF9 or rabbit normal IgG-protein A Sepharose beads (GE Healthcare, catalog number: 17-1279-02) for 2 □ hours at 4□°C ...
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bioRxiv - Immunology 2022Quote: ... Human IgG and IgA antibodies were purified from donors’ sera by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare) and peptide M-coupled agarose beads (Invivogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... or normal rabbit serum (Abclon, Seoul, Republic of Korea) was cross-linked with Protein G Mag Sepharose beads (GE Healthcare, IL, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein from each sample were added to 50 µl of precleaned CaM binding beads (GE Healthcare, 17-0529-01) in 300 to 500 µl of 1% TX100-TNM lysis buffer and incubated with rotation at 4°C overnight (16-18 hours) ...
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bioRxiv - Plant Biology 2019Quote: ... 10 µM GUN1-PS protein was subjected to UV-visible absorbance spectroscopy at room temperature in a Ultrospec 2100 pro spectrophotometer (GE Healthcare Bioscience) using 20 mM Tris-HCl buffer as a reference ...
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bioRxiv - Cell Biology 2019Quote: ... Protein expression was quantified using the Alpha Innotech software for the FluoroChemQ chemiluminescent exposure system (Alpha Innotech; GE Healthcare, London, ON, Canada) or ImageLab for ChemiDoc system (BioRad ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was neutralized by addition of 1:10 vol:vol 1M Tris pH 8 and applied to a 5 mL protein A Hitrap FF column (GE Healthcare Life Sciences) equilibrated in buffer A (100 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were further purified by size exclusion chromatography using a HiLoad 26/60 Superdex200 pg column (GE Healthcare, Chicago, United States). The purity of proteins was confirmed with SDS-PAGE and concentrations were determined photometrically ...
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bioRxiv - Immunology 2019Quote: Total IgG antibodies were captured from 2 µL of plasma using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Biophysics 2019Quote: ... TEV-protease digested proteins were passed over Ni-NTA to remove uncleaved proteins and then further purified by size exclusion chromatography using a Superdex200 column (GE Life Sciences) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH5α strain and purification of GST fusion proteins was achieved by incubation with Gluthatione Sepharose 4B matrix (GE Healthcare, Buckinghamshire, UK). Sumo proteins were kept bound to matrix ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Strep Tactin eluted protein was further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Life Sciences) in 25 mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... Visualization of the stained protein on nitrocellulose membranes was carried out by a LAS-4000 Mini luminescence image analyzer (GE Healthcare, Germany) using the ECL Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Free proteins were labeled following the manufacturer’s instructions and purified using Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2020Quote: If necessary the protein breakdown products were separated in the second chromatography step using gel filtration on a Superdex G200 column (GE Healthcare, Freiburg). PBS buffer was used for gel filtration column (137 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein sample was further purified by Size-exclusion chromatography on a HiLoad 16/60 Superdex 75 column (GE Healthcare Life Sciences) with 20 mM sodium phosphate (pH 6.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... Immuno-reactive protein bands were detected by the ECL system and visualized by imaging analyzer (ImageQuant LAS 500, GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... Rabbit sera were shipped to our laboratory where purification of IgG was performed by affinity chromatography using a 5-ml HiTrap protein A HP column following manufacturer’s instructions (GE Healthcare, Piscataway, NJ). Purified IgG protein concentration was determined by Nanodrop ND-1000 spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: ... The protein (5-6 ml) was further purified by FPLC over a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Rosenfeld et al., 2021), expressed using ExpiCHO™ Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: ... Clarified Expi293F culture supernatants were filtered and Fabs were purified by successive affinity and size-exclusion chromatography (on HiTrap Protein G and Superdex 200 Increase 10/300 GL columns, respectively, from GE Life Sciences) using an AKTA pure system.
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bioRxiv - Biochemistry 2020Quote: The supernatant fraction containing the rhGFAT2 protein (with or without tag) was subjected to purification using a Ni+2NTA affinity column (HisTrap HP 5 mL, GE Healthcare, USA). The column was equilibrated with 10 column volumes (CV ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibodies used for immunoblotting are described in Table S3 HRP-conjugated anti-rabbit secondary antibody was used (Table S3) and protein detection was performed with ECL Select Western Blotting Detection Reagent (GE Healthcare, RPN2235) on an ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... the concentrated linked-L-protein from the Q-Sepharose eluate was subsequently fractionated over a Sephadex S200 26/600 PG (GE Life Sciences) column using STD buffer (100 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The resulting proteins were purified using the AKTA start purification system through DEAE Sepharose Fast Flow packed in the XK16 column (GE Healthcare, USA), followed by another purification using Phenyl Sepharose (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... 20 ug of protein extracts for analysis of MT severing enzymes or 2.5 ug of protein extracts for analysis of tubulin proteins were separated under denaturing conditions and transferred to Amersham Protran Premium 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences) prior to blocking in 5% non-fat dried milk in TBS-T for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... containing the cleaved protein, was concentrated (Vivaspin 20, MWCO 5 kDa) and loaded onto a Superdex 75 26/60 column (GE Life Sciences) equilibrated with SEC buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then further purified by size exclusion chromatography using a HiLoad Superdex 75 16/600 gel filtration column (GE Healthcare, USA) equilibrated in 10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... was added and the secreted recombinant Spike or RBD protein in culture supernatant were harvested after 72 hours and affinity purified using HisTrap HP Column (GE Healthcare, USA) as described previously [25] ...
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bioRxiv - Neuroscience 2021Quote: ... the protein Syt C2AB (residues 143 to 421) was purified by gel filtration using a Superdex 75 column (GE Healthcare Life Sciences), as before(73) ...