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Citations for GE Life Sciences :
4451 - 4500 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The TEV-cleaved protein was recovered by second immobilised metal affinity chromatography (IMAC) and further purified by SEC (S200 16/60 column, GE Healthcare) in SEC buffer under isocratic flow ...
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bioRxiv - Immunology 2024Quote: Tested antibody (5 μg/mL for NTD and 1 μg/mL for spike) was captured on protein A sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified SARS-CoV-2 mutant spike or NTDs ...
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bioRxiv - Bioengineering 2024Quote: ... The obtained filtrate was then applied to a Ni-NTA column using a fast protein liquid chromatography (FPLC) system from GE Healthcare. The column was equilibrated with potassium phosphate buffer A (100 mM ...
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bioRxiv - Developmental Biology 2024Quote: ... The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3) and purified using glutathione Sepharose 4 B (GE Healthcare). An antibody against Ccrk was obtained by immunizing guinea pigs with purified GST-fused Ccrk.
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bioRxiv - Synthetic Biology 2024Quote: ... and those found to contain the purified protein were pooled and concentrated using a 10 kDa molecular weight cut-off Vivaspin spin column centrifugal concentrator (GE Healthcare) at 3894 x g and 4°C ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: ... Abcam)] overnight in rotating conditions followed by incubation with 50 µl of 50% Protein A Sepharose-beads (GE Healthcare,17-0780-01) for 2 hrs at 4°C in rotating condition ...
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bioRxiv - Biochemistry 2024Quote: ... for 18 h at 18°C and N-terminal His6-tag fusion proteins purified by step-wise affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM tris-HCl (pH 7.4) ...
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bioRxiv - Biochemistry 2024Quote: ... the excess unreacted dye was removed from each protein by passing it through a 600 μl Sephadex G-25 Fine resin (GE Healthcare), pre-equilibrated with a buffer containing 20 mM Hepes-K pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... The denatured proteins were resolved on 8–12 polyacrylamide-SDS gels and transferred onto PVDF membrane (GE Healthcare, Chicago, IL, USA). A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color ...
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bioRxiv - Microbiology 2024Quote: ... The protein was further purified using size exclusion chromatography with a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare) into a final buffer of 20 mM HEPES pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was separated on an 8-16% gradient Tris-glycine gels and transferred to PVDF blotting membrane (GE Healthcare Life Sciences). The membrane was immunoblotted with 1:200 anti-FGF13 antibody or 1:200 anti-pan FHF-A (Neuromab clone N235/22 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein detection and quantification were performed using the SYBR-green fluorescence detection mode of the LAS-4000 image analyzer (GE Healthcare) and Image Quant TL software (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were detected with host-specific secondary antibodies and developed with a chemiluminescent substrate (GE Healthcare Amersham, Piscataway, NJ, USA). After stripping ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Biophysics 2024Quote: ... monomeric and tetrameric protein were separated and collected by SEC using a Superdex 75 Increase 10/300 GL column (GE Healthcare) and then used directly in fluorescence experiments (SI Appendix ...
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bioRxiv - Biophysics 2024Quote: ... TEV-cleaved protein was then diluted 1:500 in KBT and then loaded onto a HiTrap Q HP column (GE Healthcare) for ion exchange chromatography (IEC ...
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bioRxiv - Plant Biology 2024Quote: ... His6-tagged proteins (His6-HsfA2/S-HsfA2/S-HsfA4c/S-HsfB1/S-HsfB2a/DBD/tDBD) and His6 mock proteins were purified using glutathione Sepharose 4B beads (GE Healthcare) and nickel-nitrotriacetic acid (Ni-NTA ...
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bioRxiv - Microbiology 2024Quote: ... Full-length untagged cGAS protein was then ultimately purified using size-exclusion chromatography via a 16/600 Superdex S75 column (GE Healthcare). Purified cGAS was concentrated to ∼10 mg/ml in storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing L–P protein were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2024Quote: ... The molecular weight of protein complexes was determined by size exclusion chromatography performed on Superdex 200 Increase 10/300 column (GE Healthcare) calibrated with High Molecular Weight calibration kit (GE Healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, PA) using AKTA pure FPLC system ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein separation was carried out with different ion exchange columns: A cation exchange column (HiScreen Capto SP ImpRes, GE Healthcare, München, Germany), an anion exchange column (HiTrap Q HP 1 ml ...
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bioRxiv - Biophysics 2019Quote: ... The protein bands were subsequently imaged using an Amersham Imager 600 instrument and its accompanying software (v0.9.8) (GE Healthcare, Chicago, IL, USA) with a 1 s exposure time ...
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... 150 μL GIT1-CC or β-Pix-CC protein sample at 50 μM was loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... Cleaved protein was separated from TEV and the His-GST tag with a Ni-NTA column and proteins were further purified by size-exclusion chromatography on Superdex 75 16/600 (GE Life Sciences) in NMR buffer (10 mM MES ...
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bioRxiv - Neuroscience 2021Quote: ... Co-IPs done to ckeck for homodimers were done the same way except that agarose beads (Protein G Sepharose™ 4 Fast Flow by GE Healthcare) were used that were incubated with mouse anti-myc antibody (9B11 ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Microbiology 2019Quote: ... Affinity-purified proteins were polished by size-exclusion chromatography (SEC) on Superdex 75 or Superdex 200 columns (GE Healthcare, Chicago, IL, USA) equilibrated with buffer B2 (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: Co-immunoprecipitations of Rps3p with Bfr1p-3HA were performed with similar conditions to Bfr1p-yeGFP but immunoprecipitated with Protein G Sepharose® 4 Fast Flow beads (GE Healthcare). Prior to the immunoprecipitations ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences) column equilibrated in Buffer B (50 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... The LytH protein was further purified using size-exclusion chromatography with a Superdex 200 Increase 10/300 GL column (GE Life Sciences) equilibrated in Buffer C (25 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... The unbound protein fraction containing biotin-PS-CFP2 was again purified via gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences). The monomeric biotin-PS-CFP2 was stored in 1 x PBS at −80 °C.
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bioRxiv - Biophysics 2019Quote: GST-fusion proteins (UBAN domains of OPTN, ABIN, NEMO and Ub) were cloned into pGEX-4T-1 (GE Life Sciences, Freiburg, Germany) and site-directed mutagenesis was performed by PCR to introduce desired amino acid substitutions.
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bioRxiv - Microbiology 2020Quote: ... The labeled protein was separated from the excess of free 125I in a PD10 desalting column (GE Healthcare Life Sciences, United Kingdom) and the purity of the 125I-labeled Vip3Aa was checked by autoradiography ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted Cavin4a-FL and Cavin4b-FL proteins were loaded on size exclusion chromatography column Superose™ 6 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 0.22 μm filtered 50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Yield can be improved by taking pooled TRAPP complex before SEC and manually running protein through a 1 mL HisTrap™ FF column (GE Healthcare) that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... TEV protease-digested proteins were passed over Ni-NTA agarose to remove uncleaved proteins and then further purified by size exclusion chromatography using a Superdex200 column (GE Life Sciences) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed once with PBS containing 0.05% Tween-20 (PBST) and then incubated with Protein A/G Sera-Mag SpeedBeads (GE Life Sciences, 17152104010350) for 1 hour at 4°C with orbital shaking ...
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bioRxiv - Biochemistry 2021Quote: Samples were dialysed overnight in the corresponding buffers and different concentrations of protein were loaded onto a size-exclusion Superdex200 Increase 3.2/300 column (GE Healthcare Life Science) at a flow rate of 50 μL/min ...
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bioRxiv - Bioengineering 2021Quote: ... The eluted protein was then buffer exchanged to 10 mM HEPES at pH 7.4 by PD-10 columns (GE Healthcare Life Sciences) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... CALM ANTH domain and Ub proteins were further purified on a HiLoad 16/60 Superdex 200 column (GE Healthcare Biosciences, Pittsburg, PA) equilibrated with 20mM HEPES-NaOH ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed using an M-110S Microfluidizer (Microfluidics) and proteins were purified using glutathione chromatography according to the manufacturer’s instructions (GE Healthcare Life Sciences). To confirm the size and purity of purified recombinant protein ...
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bioRxiv - Plant Biology 2021Quote: ... Oligomeric states of OsbZIP47 protein was determined by analytical size exclusion chromatography (SEC) performed at 4°C on a Superdex 200 increase column (GE Healthcare Inc.) pre-equilibrated with buffer (25 mM sodium phosphate (pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: The IgG fraction from the hyperimmunized rabbit serum was purified on 2x 1 mL HiTrap™ Protein G HP columns (GE Healthcare). And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... The protein was then separated by size-exclusion chromatography (SEC) using a Superdex 75 16/600 column (GE Healthcare Life Sciences, UK) equilibrated with PBS pH 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare, UK) (Nettleship et al. ...