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Citations for GE Life Sciences :
4251 - 4300 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: The binding affinity between the purified hPIV3 L-P complex protein and a RNA duplex were measured at room temperature using a Biacore S200 system (GE Healthcare) with a SA chip (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-protein binding complex was resolved on SDS-PAGE prior to analysis by Coomassie staining and PhosphoImaging (Typhoon FLA 7000, GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The protein binding complex was separated on SDS-PAGE prior to analysis by Coomassie staining and Phosphoimaging (Typhoon FLA 7000, GE Healthcare). For pull down with purified PGC-1α protein domains ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was monitored by Coomassie staining of SDS-PAGE and size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: 100 μl of protein sample was subjected to SEC at 293 K on a Superdex 200 (10/300) column (GE Healthcare) preequilibrated in 20 mM HEPES pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Simultaneously 3 μg of the anti-FLAG antibody (described above) was subjected to incubation with protein-G sepharose beads (60 ul, 17061801, GE Healthcare) for 1 hour with tumbling ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation was performed with the indicated primary antibodies and 50 μl of a 50% slurry of protein-A sepharose beads (GE Healthcare) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... the 6x His tag was cut off by the TEV protease and the protein was further purified by size-exclusion chromatography on a Superdex 75 HiLoad 26/60 column (GE Healthcare) in SEC buffer (20 mM Na2HPO4 ...
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bioRxiv - Zoology 2020Quote: ... Proteins were separated in the second dimension on a 14% SDS polyacrylamide gel (Laemmli, 1970) in a Hoefer600 cell (GE Healthcare) for 15 min at 15 mA (100 V/15 W limits ...
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bioRxiv - Immunology 2021Quote: ... was used to elute the protein using a binding buffer with 400 mM imidazole followed by size exclusion chromatography on a Superdex75 16/600 (GE Healthcare) column equilibrated in binding buffer ...
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Immunology 2020Quote: ... The soluble xfR5 mAb from the supernatant was isolated using HiTrap™ Protein-A HP prepacked column (GE Healthcare; NJ, USA) following standard manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biochemistry 2019Quote: ... Samples (100 μl) ranging in protein concentration from 15-250 μM were applied to a Superdex 200 10/300 GL column (GE Healthcare) mounted on a Jasco HPLC equilibrated in 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... All Rab proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Non-specifically bound proteins were removed by a step wash with buffer B (50 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... Protein samples (each protein at a concentration of 40 μM) were loaded to a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with assay buffer (same with ITC).
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bioRxiv - Biophysics 2019Quote: ... Dialyzed protein was diluted two-fold in buffer Q1 and loaded onto a HiTrap Q HP anion exchange chromatography column (GE Healthcare), equilibrated in buffer Q1 ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were expressed transiently in HEK-293T cells and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare) or streptactin beads (IBA ...
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Diafiltrated cell supernatant was purified by immobilized nickel affinity chromatography (5-ml fast flow crude column and ÄKTA fast protein liquid chromatography [FPLC] system; GE Healthcare) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The membranes were washed three times with TBS-T and protein bands were visualized using the CDP-star detection reagent (GE Healthcare). Intensity was detected and quantitatively analyzed by the Fluor-S MAX multi-imager system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with 240 mM imidazole and further purified over a Superdex S-75 gel filtration column (GE Healthcare) equilibrated in 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions of protein were then concentrated and loaded onto a 10/300 Superdex200 Increase gel filtration column (GE Life Sciences) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... the TTPN protein was concentrated and loaded on a size-exclusion chromatography (SEC) column (HiLoad 26/60 Superdex 75 pg, GE Healthcare). Peak fractions from the first SEC step ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... fused to lysozyme and BRD4-ET either alone or together was evaluated by injecting each purified protein onto a Superdex 75 Increase 10/300 GL Increase column (GE Healthcare) equilibrated with 20 mM HEPES 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The fractions containing Mre11 protein were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in Mre11 SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein containing elution fractions were concentrated on Amicon concentrator (100 kDa MWCO) and loaded a Superose 6 16/600 (GE Healthcare) pre-equilibrated in SEC buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein bands were visualised using ECL™ or ECL plus™ reagents and Hyperfilm® ECL (all from GE Healthcare) and detected with a CCD camera (Biorad) ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated on a 10% polyacrylamide gel and transferred to a nitrocellulose membrane (GE life sciences, 1060002) using a Pierce™ Power Station (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
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bioRxiv - Immunology 2022Quote: SEC-MALS experiments were carried out using a Wyatt Dawn Heleos II multiangle light scattering detector (Wyatt Technology) coupled to an AKTA Purifier UPC10 FPLC protein purification system and a Superdex 200 size exclusion column (GE Healthcare). Protein molecular weights of the individual peaks observed in the size-exclusion chromatograms were analyzed by static light scattering (SLS ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was further purified using size-exclusion chromatography over a Superdex S75 13/300 or 16/600 column (GE Healthcare). For Ube2k-UBC containing the Avi tag ...
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bioRxiv - Cancer Biology 2021Quote: ... were used for the protein detection and membrane images were taken using Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL).
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bioRxiv - Microbiology 2021Quote: Oligomerization of delVirB4_CTn4 and delVirD4_CTn4 proteins was studied by gel chromatography on Superdex200 10/30 GL column connected to ÄKTA Explorer liquid chromatography system (GE Healthcare). The proteins ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was further purified by a HiLoad® 16/600 Superdex® 200 pg gel filtration column (GE Healthcare) in buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted protein was loaded onto a size exclusion chromatography column (Superdex 75 16/60 or Superdex 200 16/60 (GE Healthcare) depending on protein size ...
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bioRxiv - Bioengineering 2021Quote: ... pre-equilibrated with five volumes of imidazole binding buffer (PBS, 10 mM imidazole buffer, pH 7.4) using a fast protein liquid chromatography (FPLC) system (AKTA Pure; GE Healthcare systems). The column was washed with twenty bed volumes of binding buffer (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... All purification steps were carried out at 4°C using the ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE healthcare). Protein purity was assessed by SDS-PAGE ...
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bioRxiv - Biophysics 2020Quote: ... Uncleaved protein and cleaved hexahistidine tag were removed from the eluted mixture with a HisTrap HP 5 mL column (GE Healthcare) in PBS with 25 mM imidazole ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysate equivalent to 1 mg protein collected 36 hours post-transfection was diluted to 500 μl in TGH buffer for each immunoprecipitation reaction and precleared with 10 μl sepharose-protein G beads (GE Healthcare). Immunoprecipitation was performed overnight by incubation with anti-V5 antibody ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions containing desired proteins were pooled and further purified by size exclusion chromatography (HiLoad 16/60 Superdex 200 column, GE Healthcare) using sodium phosphate buffer A as eluent ...
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bioRxiv - Immunology 2020Quote: ... Purified mAbs were first immobilized at coupling densities of 800-1200 response units (RU) on a series S sensor chip protein A (GE Healthcare) in PBS and 0.02% sodium azide buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins in cell lysates were separated on SDS-PAGE and then transferred electrophoretically onto PVDF membranes (GE Healthcare, Milwaukee, WI, USA) using a transfer cell (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... at room temperature and immunoprecipitated protein was detected using Amersham® ECL Plus Western Blot Detection System (GE Healthcare, Buckinghamshire, UK). Radioactivity ...
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bioRxiv - Microbiology 2019Quote: ... clarified lysates were mixed with 1 µg anti-HA monoclonal antibody and rotated with 30 µl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 3 hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... All antibody formats were expressed using ExpiCHOTM Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE.