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Citations for GE Life Sciences :
401 - 450 of 640 citations for Radio Flow Detectors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The flow-through containing GTPBP6 was collected and applied to a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in size exclusion buffer (70 mM NH4Cl ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate is incubated with 100ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) overnight in rotator wheel at 4°C with slow rotation ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through was then applied to a HiTrap Q HP 5 mL anion exchange chromatography column (GE Healthcare) attached to an ÄKTA Explorer 100 system (Amersham Pharmacia Biotech) ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting sNASPc–H3–H4–ASF1b heterotetramer was first purified by Ni Sepharose 6 Fast Flow beads (GE Healthcare). After removal of the His6-SUMO tag by home-made Ulp1 (SUMO protease) ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through was further purified by cation-exchange chromatography on a 5 mL HiTrap S column (GE Healthcare) with a salt gradient of 0 to 1 M NaCl in 1×PBS supplemented with 1 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 0.5 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2-3 hours on a roller ...
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bioRxiv - Biophysics 2020Quote: ... 10% glycerol (including 100 mM NaCl and 0.5 mM DTT for PSG)) Nickel Sepharose Fast Flow column (GE Healthcare). Proteins were eluted with 250 mM imidazole and dialysed overnight into 50 mM Tris pH 7.8 (PDZ3 ...
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bioRxiv - Microbiology 2021Quote: ... and the membrane extract was loaded on a Chelating Sepharose Fast Flow bed resin (∼10 ml; GE Healthcare, UK) previously activated with 200 mM NiCl2 (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... proteins were affinity purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). Supernatant was two-fold diluted with 1xPBS (pH 7.4 ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2μm) and loaded with a flow rate of 1ml/min onto a Source 15S 4.6/100PE column (GE Healthcare) equilibrated with buffer A (50 mM MES ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was purified with a cation exchange column (self-packed with SP Sepharose Fast Flow resin, GE Healthcare) then further purified with a reverse-phase HPLC equipped with ZORBAX 300SB-C3 column (MeCN gradient from 5-50% over 45 min) ...
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bioRxiv - Molecular Biology 2023Quote: ... The general purification strategy was as follows: (i) Nickel (II) affinity chromatography (Ni Sepharose 6 Fast Flow, GE Healthcare) in 25 mM Tris (pH 8.0) ...
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bioRxiv - Biophysics 2023Quote: ... Protein was isolated from the resulting supernatant by affinity chromatography using Ni-Sepharose 6 Fast Flow beads (GE Healthcare), and eluted with a 100 mL linear gradient of column buffer with 500 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of media was concentrated over 2 ml heparin affinity medium (Heparin Sepharose 6 Fast Flow, GE Healthcare) packed into a 10 ml Poly-Prep Chromatography gravity flow column (Bio-Rad) ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was purified with a cation-exchange column (self-packed with SP Sepharose Fast Flow resin, GE healthcare) running between 20 mM Na2HPO4 (pH 6.8) ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was incubated for 45 minutes with 20 µl of Ni Sepharose 6 Fast Flow beads (GE Healthcare), washed and resuspended in 100 µl of buffer A (30 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was loaded onto a Ni2+ Sepharose™ 6 fast-flow agarose column (GE Healthcare, Waukesha, WI, USA) for purification ...
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bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare) for 2 hours at 4°C in a rotating wheel ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting flow-through was concentrated and run on a HiLoad 16/60 Superdex 200 column (GE Life Sciences) into crystallization buffer ...
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bioRxiv - Biophysics 2021Quote: ... 500 µL sample was injected and run with a flow rate of 0.5 mL/min on an ÄKTA Pure protein purification system (GE Healthcare). Control samples containing ThT alone at the lowest (0.4 M urea ...
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bioRxiv - Microbiology 2020Quote: ... The cleared membrane fraction was loaded with a flow of 0.8 mL/min on a pre-equilibrated 5 mL StrepTrap column (GE healthcare) mounted on as AktaPurifier system (GE healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... 5% glycerol (w/v, 0.1% Triton X-100) and mixed with 50 µl Glutathione Sepharose 4 Fast Flow (GE Healthcare) slurry ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrate was separated with a flow rate of 0.5 ml/min on a Superdex 200 10/300 GL column (GE Healthcare, Little
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bioRxiv - Molecular Biology 2019Quote: ... The flow-through fraction was further purified by HiTrap Q FF column and Superdex 75 16/60 column (GE Healthcare). The pooled fractions were concentrated to 35 mg/ml (UBA-linker-helix ...
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bioRxiv - Biophysics 2020Quote: ... The fractions containing pure OmpLA were pooled and further passed through a 1ml HiTrap Q-Sepharose fast flow (GE healthcare) anion-exchange column equilibrated with the same buffer ...
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Bioengineering 2021Quote: The yielded supernatant was passed through a 0.2 μm filter and loaded with a flow rate of 0.5 mL/min on a 5 mL HisTrap HP column (GE Healthcare) equilibrated with 12 CV binding buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Microbiology 2020Quote: ... Flow-through was concentrated and passed over a SEC column (HiLoad 16/600 Superdex 200 pg; GE Healthcare Life Sciences) equilibrated in buffer C (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Microbiology 2020Quote: ... then 1.5 column volume of mobile phase (DPBS) was run through the column at flow rate of 0.5 mL/min using the AKTA Pure (GE Healthcare) system ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin was then precleared overnight at 4°C with vertical rotation at 15rpm (a total of 5 reactions per condition in 15ml tubes) with 500µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) beads and then used for immunoprecipitation.
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bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
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bioRxiv - Molecular Biology 2020Quote: ... The homogenate was cleared by centrifugation at 30,000g and loaded onto a gravity-flow column containing Glutathione Sepharose 4B (GE Healthcare). The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted with a 20 ml linear gradient from 150 mM to 500 mM at a flow rate of 0.5 ml/min using an ÄKTA Pure instrument (GE Healthcare). The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore ...
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bioRxiv - Biophysics 2021Quote: ... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... 0.05% NP-40) and assembled complexes were affinity purified from the cushions using lgG Sepharose 6 Fast Flow (GE Healthcare) to capture the Protein A tag ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Biochemistry 2022Quote: The purified protein was used for size-exclusion chromatography with a flow rate of 0.8 mL/min (HiLoad 16/60 Superdex 75 pg column; GE Healthcare) to evaluate the molecular weight of various recombinants Pvs25 ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... using a Superdex 200 Increase 10/300-Gl column with a flow rate of 0.3 ml/min on an Äkta purifier (GE Healthcare). Four milligrams of protein was loaded in a volume of 0.4 ml (8,7 mg/ml) ...
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bioRxiv - Immunology 2020Quote: ... Background binding to blank immobilized flow cells was subtracted and affinity constants were calculated using BIA Evaluation software (GE Healthcare) using the Steady State Affinity model ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were pelleted and lysed by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 500 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... the flow-through was buffer exchanged to PBS and concentrated using Vivaspin filters (10 kDa molecular weight cutoff; GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: PeSkC and RoSBC samples were applied to a 1.8×13cm Sephadex G100 column at a flow rate of 1ml min-1 using an ÄKTAprime plus unit (GE Healthcare). Void volume was calculated by running blue dextran ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was purified to homogeneity by column chromatography employing Q-Sepharose Fast Flow (GE Healthcare Life Sciences, Buckinghamshire, UK), Hydroxyapatite (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2022Quote: ... Polyclonal IgG from subject 391370 was isolated from heat-inactivated plasma using Protein G Sepharose 4 Fast Flow (GE Healthcare). Purified IgG was buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS ...