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Citations for GE Life Sciences :
451 - 500 of 640 citations for Radio Flow Detectors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... the concentrated protein was gel-filtrated using a Superdex 200 10/300 GL column equilibrated in HBS at a flow rate of 0.5 ml/min and 4 °C (GE Healthcare). Peak fractions at a final OD280nm of ∼ 0.4 (∼ 1.4 μM ...
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bioRxiv - Cell Biology 2019Quote: ... and for additional 4 hours with 40 µl of pre-washed Protein G Sepharose 4 Fast Flow beads (GE Healthcare) in a rotating wheel ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was filtered and added to a pre-equilibrated (50 mM Tris, pH 7.8, 10% glycerol) Nickel Sepharose Fast Flow column (GE Healthcare). Proteins were eluted with 250 mM imidazole and dialysed into 50 mM Tris pH 7.8 ...
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bioRxiv - Biochemistry 2020Quote: ... and flow-through containing the cleaved SALM3-LRR was collected and gel filtered with Superdex 75 10/300 (GE Healthcare) in 60 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis tagged protein was purified by IMAC affinity purification using 0.5 ml of packed Sepharose 6 Fast Flow Ni-NTA Resin (GE Healthcare) pre-washed with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate is then incubated overnight with 400ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) at 4°C with slow rotation ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Ab Spin Trap columns (immobilized protein G) and NHS-activated sepharose® 4 fast flow were purchased from GE Healthcare Life Sciences (Buckinghamshire ...
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bioRxiv - Synthetic Biology 2020Quote: ... The extracellular media was then diluted two-fold with 20 mM HEPES buffer at pH 7.0 and applied to 1 column volume (CV) of DEAE Sepharose Fast Flow Resin (from GE Healthcare) equilibrated with 10 CV of 20 mM HEPES buffer at pH 7.0 (1 CV equaled 2 ml of settled resin for 25 ml cultures and 1 CV equaled 4 ml of settled resin for 250 ml cultures) ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 33,000 g and loaded on pre-equilibrated Ni-Sepharose 6 Fast Flow (HisTrap FF, GE Healthcare). The column was washed with 20-30 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... and lysates were clarified by centrifuging at 30,000g for 30 minutes and the supernatant was incubated with Ni resin (Ni Sepharose Fast Flow, GE Healthcare) for 30 minutes at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and the flow-through was purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare). Purified samples were stored in gel filtration buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Polyclonal anti-GST antibody (Carl Roth, 3998.1) was covalently immobilized to all four flow cells of a CM5 sensorchip (GE Healthcare) to a level of 8,000 response units (RU ...
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bioRxiv - Biophysics 2020Quote: ... 3mM CaCl2 and 5mM Imidazole pH 8.0 and incubated with Ni2+ charged IMAC Sepharose 6 Fast Flow resin (GE Healthcare) for 1 hr at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The flow-through fraction was further purified by HiTrap Q FF column and Superdex 75 16/60 column (GE Healthcare). The pooled fractions were concentrated to 20 mg/ml in crystallization buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... We pelleted and lysed bacteria by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 0.5 M imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred to ice cold 15 ml tubes containing 1ml of IgG Sepharose 6 Fast Flow (GE Healthcare) and incubated on a rotating platform for 3 hours at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2 μm) and loaded with a flow rate of 1 ml/min onto a Source 15S 4.6/100PE column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... or IgG as a control were immobilized on 50 μl of Protein A–Sepharose 4 Fast Flow beads (GE Healthcare) for 3 h and incubated with clarified lysates.
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bioRxiv - Molecular Biology 2023Quote: ... samples were dialyzed against 25 mM Tris (pH 8.0) and loaded onto a Q column (HiTrap Q Fast Flow, GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Column flow-through was concentrated via centrifugal concentrator (10k MWCO) and applied to a Superdex 200 10/300 (GE Healthcare) gel filtration column pre-equilibrated in 50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... were injected at flow rate of 0.4 ml min−1 to a Superdex 75 Increase 10/300 GL column (GE Healthcare) equilibrated with buffer containing 20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... The column was run at a flow rate of 0.5 mL min-1 on an AKTAbasic liquid chromatography system (GE Healthcare) coupled to a miniDawn MALS detector and an Optilab refractive index detector (Wyatt Technology) ...
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bioRxiv - Microbiology 2024Quote: ... Flow-through containing the untagged proteins was concentrated and loaded into a Superdex-200 10/300 GL column (GE Healthcare) pre-equilibrated with storage buffer ...
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bioRxiv - Microbiology 2024Quote: ... The soluble fraction was loaded onto a Ni Sepharose™ 6 fast flow agarose column (GE Healthcare, Waukesha, WI, USA), and the protein was eluted using a buffer (pH 7.9 ...
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bioRxiv - Biochemistry 2023Quote: ... gel filtration with a flow rate of 0.8 mL/min using FPLC systems (AKTA pure 25, GE Healthcare Life Science). The 5 mL sample concentrated from the fractions involving the target proteins with Amicon Ultra-15 10 kDa was loaded on Resource Q (GE Healthcare Life Science ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ml antibody-containing ascites was incubated with 1.2 ml settled bead volume of pre-equilibrated Protein G Sepharose Fast Flow beads (GE Healthcare) for 2 hr at 4°C with gentle agitation ...
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bioRxiv - Biophysics 2020Quote: ... A second affinity column with Ni-NTA agarose was performed and the flow-through was loaded into a Superdex 75 column (GE Healthcare) for size exclusion chromatography in buffer A.
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bioRxiv - Immunology 2020Quote: ... CR2-Fc and FH19-20 were prepared in 10 mM sodium acetate pH 5 and immobilised at 300 RU (CR2-Fc: 20 µM, FH19-20: 240 µM) to different flow cells of CM5 chips (GE Healthcare) using standard amine coupling involving preparation of the dextran matrix with 100 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and addition of 40 µl Protein A Sepharose resin (nProtein A Sepharose 4 Fast Flow, 17-5280, GE Healthcare) that had been blocked for 1 h in ChIP Blocking buffer (ChIP Low salt buffer containing 2 mg/ml BSA and 2% (w/v ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate was clarified by centrifugation at 35,000g and purified by immobilized metal affinity chromatography (IMAC) on a 5 mL Fast Flow Chelating Sepharose column (GE Life Sciences) with an elution gradient from 0 to 200 mM imidazole over 120 mL ...
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bioRxiv - Biochemistry 2019Quote: ... and purified using a standard step gradient (300 mM imidazol) immobilized metal affinity chromatography (IMAC) protocol (Ni-Sepharose Fast Flow, GE Healthcare). Afterwards ...
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bioRxiv - Molecular Biology 2019Quote: ... and trimeric complexes were affinity purified via 6xHis-tagged RING1B on Ni2+-charged IMAC Sepharose 6 Fast Flow resin (GE Healthcare). 10 mM imidazole was added to lysates during binding ...
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bioRxiv - Biochemistry 2019Quote: ... The obtained dialyzed sample was then subjected to a second round of ultracentrifugation and the supernatant was loaded onto the cation exchange column (Sepharose fast flow GE healthcare) for further purification ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing the soluble protein fraction was loaded onto a diethylaminoethyl ion-exchange column (DEAE-Sepharose Fast-Flow, GE Healthcare), followed by a Nickel-ion affinity column (Chelating-Sepharose Fast-Flow ...
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bioRxiv - Biophysics 2019Quote: ... the flow through from this step was concentrated using a centrifugal concentrator and applied to a Superdex200 10/300 GL (GE Healthcare) size exclusion column equilibrated with a “Standard” buffer of 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through that contained the untagged protein of interest was applied to an ion exchange chromatography HiTrap Q column (GE Healthcare). The unbound protein sample ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were purified at 4°C on the anti-FLAG resin at a flow rate of 20 ml/h with a P1 pump (GE Healthcare), and eluted by competition with a FLAG peptide solution at 200 μg/ml in 10 mM Hepes ...
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bioRxiv - Biochemistry 2020Quote: The binding affinity of FN3Con-anti-VEGFR2 was measured using surface plasmon resonance with a 30 µl/min flow rate at 25 °C (BIAcore T-100, GE Healthcare). VEGFR2 domains (SiboBiological ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation at 18000*g for 45 min at 4 °C and the supernatant was applied to a Ni2+–Sepharose 6 Fast Flow (GE Healthcare) loaded gravity column ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved KIX was purified using a linear gradient of 50 mM to 1 M NaCl over ten column volumes by cation exchange chromatography using MonoS 5/50 GL column at a flow rate of 1 mL per minute (GE Healthcare). Fractions containing purified KIX were confirmed by SDS-PAGE and dialyzed against 1 L of 50 mM potassium phosphate pH 5.5 ...
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bioRxiv - Biophysics 2021Quote: ... The flow through containing SARS-CoV-2 Mpro was collected and further purified using size exclusion chromatography column (G-100, GE Healthcare,) equilibrated with 20 mM Tris ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed 6×His tagged ARL3 and its variants were purified with Ni SepharoseTM 6 Fast Flow beads (GE Healthcare) and cleaved with thrombin (Solarbio ...
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bioRxiv - Biochemistry 2020Quote: ... ChIP for Rat1-TAP was performed by incubating 500 μl of the solubilized chromatin sample with 20 μl of IgG Sepharose 6 Fast Flow (GE healthcare) for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The full length hTop2α was first purified by a tandem affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE healthcare) bound to Ni2+ ions followed by a StrepTrap HP column (GE healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol and 1 mM DTT prior to ion exchange chromatography using a Fast Flow SP column (GE Healthcare). Elution from the SPFF column was performed with a 100 mM to 1M gradient over 20 column volumes ...
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bioRxiv - Microbiology 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare). Nla28-DBD was eluted using 100 ml cold column buffer containing 10 mM maltose ...