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Citations for GE Life Sciences :
401 - 450 of 923 citations for PD L1 Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The buffer was exchanged with PBS by size exclusion chromatography using a PD-10 Desalting Column (GE Healthcare). The purity of EqPD-1-Ig and EqPD-L1-Ig was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions corresponding to pure RIP2K were then pooled and applied onto a PD-10 desalting column (GE Healthcare) equilibrated with buffer C (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: RIP2K1-317 buffer exchange in buffer F was done by using a PD-10 desalting column (GE healthcare). 5mM MgCL2 and 2 mM ATP were added to the kinase sample ...
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bioRxiv - Biochemistry 2022Quote: ... as previously described.[58] Desalting was carried out using a PD-10 column (GE Healthcare Life Sciences, UK). For PCMjMAT the protein was further purified by heating at 80 °C for 20 min followed by 30 min centrifugation at 18213 × g (Eppendorf centrifuge 5427 R) ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was purified using size-exclusion chromatography (PD MidiTrap G-25, GE Healthcare Bio-Sciences, gravity protocol) and stored at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Buffer exchange was performed using 5E10 VPs and PD-10 MiniTrap G-25 columns (GE Healthcare, Solingen, Germany) following the companies instructions with the “gravity” protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fab were separated from unconjugated dye in an equilibrated PD MiniTrap G-25 desalting column (GE Healthcare). Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and the buffer was exchanged with ultrapure water ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and buffer was exchanged for ultrapure water ...
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bioRxiv - Plant Biology 2023Quote: ... Protein containing fractions were pooled and buffer exchange was carried out using a PD-10 column (GE Healthcare) using TES-KCl buffer (25 mM TES/KOH pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Imidazole and NaCl were removed using a PD-10 desalting column according to the manufacturer’s instructions (GE Healthcare) at 4 °C and the concentration was determined by spectrometry (NanoDrop Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... samples were desalted into storage buffer (50mM Tris [pH 8.0], 0.2M NaCl, 0.5mM EDTA) using PD MiniTrap Sephadex G-25 columns (GE Healthcare). Otherwise ...
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bioRxiv - Systems Biology 2023Quote: ... followed by DTT removal using a PD-10 desalting column with Sephadex G-25 resin (from GE healthcare). The reduced protein was mixed with a ten fold excess of the nitroxide spin label MTSL and incubated at room temperature protected from light for one hour ...
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bioRxiv - Biophysics 2020Quote: ... the supernatant was collected for purification using His Trap Excel column (GE Healthcare). The proteins were labeled with an AlexaFluor dye through the ybbR-tag and gel-filtered via a Superdex 75 10/300 GL column ...
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bioRxiv - Cell Biology 2019Quote: ... His-NF1 GAP was purified using a HisTrap excel-affinity column (GE Healthcare) and eluted in buffer containing 50mM Tris ...
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bioRxiv - Microbiology 2020Quote: His-tagged 2019-nCoV S was immobilized to an NiNTA sensorchip (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Neuroscience 2020Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded on a Ni-NTA His-trap column (GE healthcare), washed with 10 column volume of buffer A containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were loaded on Hi-trap Q HP (GE Healthcare, cat.17115401) anion exchange column ...
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bioRxiv - Cell Biology 2020Quote: MCAK was first affinity purified by binding to His-Trap HP (GE Healthcare) in binding buffer (50mM NaPO4 ...
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bioRxiv - Molecular Biology 2020Quote: ... and lysates were cycled over a 5 ml His-Trap column (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for GST-tagged proteins at 4 °C following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were passed over a 1 ml His-Trap HF column (GE Healthcare). The bound protein was washed with 15 ml high salt wash buffer (20 mM potassium HEPES ...
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bioRxiv - Biophysics 2019Quote: ... further loaded onto a His-Trap HP column (GE Healthcare, Chicago, IL, USA), washed and then eluted in gradient with the same buffer supplemented with 250 mM imidazole pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: IMAC purification was performed using a His-Trap HP 1 ml (GE Healthcare) on an ÄKTA START protein purification system (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was purified by His Trap™ FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
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bioRxiv - Microbiology 2021Quote: ... The sera were purified on Hi-Trap Protein G HP columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and applied to a 1-ml chelating Hi-Trap Sepharose column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Neuroscience 2019Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Biochemistry 2019Quote: ... The dialyzed protein extract was purified via a His-trap column (GE Healthcare) previously equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... RNase HI was loaded onto a 16/600 Superdex 75 column (GE Healthcare), whilst PolI and Ligase were further purified on a 16/600 Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto a 3ml His SpinTrap™ column (GE Healthcare) previously charged with Ni2+ and equilibrated in lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... lysate was affinity purified using Cobalt-charged His-Trap Chelating column (GE Healthcare) through imidazole gradient elution ...
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bioRxiv - Immunology 2020Quote: ... Harvested supernatants were applied to a Hi-Trap Nickel excel columns (GE Healthcare) using an Akta pure (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... Nbs from PE were purified using IMAC Hi-Trap columns (GE Healthcare, 17092003) following manufacturer’s instructions and eluted using 300 nM Imidazole ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged Prtg protein was labeled directly with Cy5 monoreactive dye (GE Healthcare). Each bait protein was screened in duplicate against a total number of 1241 secreted proteins distributed across two microarray slides ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to a 5 ml His-TRAP column (GE Healthcare) and eluted with a gradient of 20 mM to 500 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... All the His-tagged proteins were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100 mM-500 mM Imidazole gradients ...
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bioRxiv - Microbiology 2023Quote: ... The F341_RBP was purified using Ni2+- NTA-affinity chromatography (His GraviTrap, GE Healthcare) according to manufacturer’s instructions and exchanged into PBS buffer using 10 kDA Amicon® Ultra-15 Centrifugal filter units ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was loaded onto a nickel-chelated Hi-trap column (GE Healthcare) and eluted with a linear gradient of 25-500 mM imidazole in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... HIS-p300 was further purified on a Q sepharose column (HiTrap, GE Healthcare) in BC buffer and eluted between 100-600 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-his was purified using a HiTrap IMAC FF Ni2+ (GE Healthcare) affinity column and Superdex 75 10/300 size exclusion chromatography (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... Concentrated fraction was mixed with another fraction and was loaded into PD-10 desalting column (GE Healthcare Lifescience, 17085101) for buffer exchange with incubation buffer containing 50 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... the preparation was diluted in PBS and applied to a Sephadex G-25 column in PD-10 (GE Healthcare). Particle concentration and size distribution of the liposomes generated were evaluated using the NanoSight NS300 instrument (Malvern ...
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bioRxiv - Plant Biology 2020Quote: For NMR structure determination the proteins were buffer exchanged using PD-10 columns (Sephadex G-25 M, GE Healthcare) or via gel filtration chromatography in the final purification step to 20mM MES 6.5 ...
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bioRxiv - Neuroscience 2020Quote: ... and loaded on a equilibrated G-25 column (Column PD Minitrap G-25, GE Healthcare, Cat.# 28-9180-07). Once the solution fully entered the column ...
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bioRxiv - Biochemistry 2020Quote: Lipoproteins secreted into the basolateral media were separated from free tracers using a PD-10 desalting column (GE Healthcare) as described previously [23] ...
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bioRxiv - Genomics 2021Quote: ... DNA from the isolated pooled LL and HL fractions was desalted on PD MiniTrap G-25 columns (GE Healthcare) equilibrated in 10m Tris-Cl pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... LUVs were subjected to size exclusion chromatography on a sephadex G25 PD-10 column (GE Life Sciences, Chicago, Il). The concentration of the encapsulated Indo-1 was estimated using fluorescence and known amounts of free Indo-1 ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were pooled for buffer exchange employing a PD-10 Sephadex™ G-25 desalting column (GE Healthcare) and centrifugal filters (Amicon®Ultracel®-10 K/30 K ...