Labshake search
Citations for GE Life Sciences :
351 - 400 of 923 citations for PD L1 Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Protein was captured on Nickel columns (His Trap HP, GE Healthcare) and eluted with 500 mM Imidazole ...
-
bioRxiv - Biochemistry 2023Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare). Histones were eluted by a salt gradient from 200 to 600 mM NaCl ...
-
bioRxiv - Cell Biology 2019Quote: ... 1 mM EGTA) supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare). Drosophila KLP10A (plasmid pGG885 ...
-
bioRxiv - Microbiology 2019Quote: ... Excess reducing agents were removed by running the protein solution through a PD-10 column (GE Healthcare) equilibrated with 20 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: ... Fractions containing the purified protein were pooled and desalted with PD midi Trap G-25 (GE Healthcare) using the spin protocol ...
-
bioRxiv - Neuroscience 2019Quote: ... The coupling reaction was filtered through a PD MiniTrap G-25 column (GE Healthcare #28-9180-07) to remove the excess of BG ...
-
bioRxiv - Biophysics 2021Quote: ... separated from free dye by gel filtration using a PD-10 column (GE Life Sciences, Chicago, IL), and diluted to a working lipid concentration of 144 μM ...
-
bioRxiv - Biochemistry 2020Quote: ... Fractions containing protein of interest were collected and desalted using PD-10 midi desalting columns (GE Healthcare). Protein of interest was eluted with the storage buffer (50 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: ... sortase and excess DBCO-amine was removed using Ni-NTA resin and PD-10 columns (GE Healthcare) respectively ...
-
bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
-
bioRxiv - Biophysics 2021Quote: ... Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare, Marlborough, MA).
-
bioRxiv - Immunology 2022Quote: ... followed by Ni-NTA purification and desalting by PD MiniTrap G-25 columns (GE Healthcare Life Sciences).
-
bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged by passing the eluate through a desalting PD-10 column (GE Healthcare, Chicago, IL), equilibrated with 50 mM HEPES/KOH ...
-
bioRxiv - Microbiology 2019Quote: ... The labelled antibodies were purified by passing through Disposable PD 10 Desalting Columns (GE Healthcare Life Sciences), concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore ...
-
bioRxiv - Plant Biology 2019Quote: ... one of the 1 mL supernatant was desalted by PD-10 desalting columns (GE Healthcare, Pittsburgh, PA) according to manufacturer’s instructions and eluted with 3 mL IP buffer ...
-
bioRxiv - Microbiology 2019Quote: ... Dual-labeled capsid proteins were separated using a PD Mini-Trap G-25 desalting column (GE healthcare) with imaging buffer (50 mM Tris ...
-
bioRxiv - Biochemistry 2021Quote: ... pH 8 supplemented with 0.22 mM DDM by gel filtration using the PD-10 column (GE Healthcare). Samples were concentrated using Amicon®Ultra-4 ultrafiltration device ...
-
bioRxiv - Molecular Biology 2022Quote: ... the pellet was solved in lysis buffer and transferred onto a PD-10 desalting column (GE Healthcare). Purification was then carried out essentially according to the manufacturer’s protocol using elution buffer (lysis buffer with 250 mM imidazole ...
-
bioRxiv - Microbiology 2022Quote: ... Virus stocks were cleared of residual EdC using a PD-10 desalting column (GE Healthcare, Mississauga, ON) utilizing the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... The reaction was quenched with ∼650 μM β-mercaptoethanol and desalted using PD-10 columns (GE healthcare). The extent of reaction was checked using 1D intact weight ESI mass spectrometry (SIRCAMs ...
-
bioRxiv - Biochemistry 2024Quote: ... size exclusion chromatography was followed by glycerol removal using a PD MiniTrap G-25 column (GE Healthcare) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... The supernatant was fractionated using a Hi-Trap Ni2+ column (GE Healthcare) following the manufacturer’s instructions and checked by SDS-PAGE electrophoresis ...
-
bioRxiv - Molecular Biology 2019Quote: Purification was performed at 4 °C using His GraviTrap columns (GE Healthcare). Equilibration was done with 10 ml of B-PER II before extracted soluble fractions were applied to the column ...
-
bioRxiv - Bioengineering 2021Quote: ... followed by purification on a His-Trap HP affinity column (GE Healthcare) using a gradient from 10 to 250 mM imidazole over 30 min in Tris buffered saline pH 7.5 in the presence of 1 mM betamercaptoethanol (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2020Quote: ... the protein was passed through a Hi-Trap desalting column (GE Healthcare) and an S75 gel filtration column equilibrated in buffer containing 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2020Quote: ... the protein was desalted using a Hi-Trap desalting column (GE Healthcare). After desalting ...
-
bioRxiv - Neuroscience 2020Quote: ... applied to Ni2+ ion affinity chromatography His Trap FF column (GE Healthcare), and purified using an Äkta pure chromatography system (GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: ... to a 5 mL His-trap crude FF column (GE Life Sciences). This was washed with Buffer A (30 mM Tris-HCl pH ...
-
bioRxiv - Microbiology 2021Quote: ... and loaded on 1 ml His-Trap columns (GE Healthcare, Freiburg, Germany), pre-equilibrated with ten column volumes of each ddH2O and sodium phosphate buffer A (20 mM Na2HPO4 * 2 H2O ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The supernatant was then run through an equilibrated His-trap (GE healthcare) in an AKTA start fast protein liquid chromatography system ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatant was loaded onto a 5mL His-trap column (GE Healthcare) for protein purification and eluted with a linear imidazole (20 mM-200 mM imidazole ...
-
bioRxiv - Microbiology 2022Quote: ... the PCR reactions were loaded on Hi-Trap Streptavidin column (GE Healthcare). By addition of 60 mM NaOH ...
-
bioRxiv - Biochemistry 2023Quote: ... Supernatants were loaded in a 1-ml His-Trap column (GE Healthcare) previously equilibrated with buffer H ...
-
bioRxiv - Cancer Biology 2024Quote: ... The supernatant was purified by His TrapTM FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Buffer was added to the supernatants to a final concentration of 0.01 M HEPES/0.2 M NaCl (pH 7.4) and passed through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The Fab were separated from unconjugated dye in an equilibrated PD MiniTrap G-25 desalting column (GE Healthcare). Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa ...
-
bioRxiv - Cancer Biology 2019Quote: ... and eluted material exchanged into PBS with Sephadex G-25 in PD-10 Desalting Columns (GE Life Sciences).
-
bioRxiv - Molecular Biology 2019Quote: ... were concentrated using a 10,000 MWCO centrifugal filter (Amicon) and desalted using a PD-10 column (GE Healthcare) equilibrated in Ulp1 cleavage buffer (20 mM HEPES ...
-
bioRxiv - Cancer Biology 2020Quote: ... unbound Alexa Fluor 488 NHS Ester was removed with a PD-10 desalting column (GE Healthcare, Piscataway, NJ) using NH4OAc buffer (0.15 M ...
-
bioRxiv - Bioengineering 2021Quote: The purified protein was desalted in deionized Milli-Q water using a PD-10 column from GE healthcare and concentrated fivefold using a Thermo scientific three kDa MWCO protein concentrator ...
-
bioRxiv - Bioengineering 2020Quote: ... Unlabelled radiometal complexes were removed by separating the vesicles with PD-10 columns (GE Healthcare Life Sciences™). Both vesicles were concentrated using Pierce™ Concentrators (30 kDa ...
-
bioRxiv - Cell Biology 2020Quote: ... the labeled protein was separated from the solution by size exclusion chromatography using PD MiniTrap G25 (GE Healthcare). PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB ...
-
bioRxiv - Biochemistry 2021Quote: ... Residual NaCO3 from the reverse phase were removed using a PD-10 desalting column (GE Healthcare Life Sciences). Before aggregation ...
-
bioRxiv - Biophysics 2020Quote: ... excess FluoZin-1 dye was removed by passing the sample through a PD-10 desalting column (GE Healthcare) pre-equilibrated with reconstitution buffer.
-
bioRxiv - Biochemistry 2022Quote: ... followed by further separation from small molecules by passage through a pre-packed PD-10 column (GE Healthcare). Protein concentrations were determined by absorbance at 280 nm using a calculated molar extinction coefficient (ε280nm ...
-
bioRxiv - Cell Biology 2020Quote: ... The labeled antibody was separated from free Cy7 using a PD-mini G-25 desalting column (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS ...
-
bioRxiv - Biochemistry 2022Quote: Ni-NTA eluates were thawed on ice and desalted via the PD-10 desalting column system (GE Healthcare) following the manufacturer’s gravity flow protocol ...
-
bioRxiv - Pathology 2020Quote: ... free TPEN and free Zn were further removed by gel filtration on PD MidiTrap G-10 (GE Healthcare) equilibrated with 40 mM of the metal-free HEPES-KOH ...
-
bioRxiv - Microbiology 2019Quote: ... The elution fractions containing the purified protein were loaded onto a PD MidiTrap G-25 column (GE healthcare) equilibrated with 20 mM phosphate buffer (pH 7.4 ...
-
bioRxiv - Immunology 2020Quote: ... 500 mM NaCl using a gravity-flow chromatography with a PD-10 desalting column (GE Healthcare Life Sciences). Buffer-exchanged RBD was concentrated to 5 mL and covalently coupled to a 5 mL HiTrap NHS-activated Sepharose column (GE Healthcare Life Sciences ...