Labshake search
Citations for GE Life Sciences :
401 - 450 of 1130 citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... and then detected with sheep anti-mouse IgG-HRP (GE Healthcare, Little Chalfont, UK; NA931V, 1:5,000), and donkey anti-rabbit IgG-HRP (GE Healthcare ...
-
bioRxiv - Molecular Biology 2022Quote: ... the membrane was incubated for 1 h at room temperature with anti-mouse (1:5000, GE Healthcare), anti-rabbit (1:5000 ...
-
bioRxiv - Neuroscience 2022Quote: ... the membranes incubated with an anti-mouse HRP-linked secondary antibody (1:5000, GE Healthcare, NXA931, RRID:AB_772209). Either Pierce ECL Western Blotting Substrate (Thermo Fisher ...
-
bioRxiv - Neuroscience 2023Quote: HRP-conjugated secondary antibodies raised against the appropriate species were used: anti- mouse IgG (GE Healthcare, #NA931), anti-rabbit IgG (GE Healthcare ...
-
bioRxiv - Cell Biology 2022Quote: ... Blots were incubated with horseradish peroxidase (HRP)-coupled sheep anti-mouse IgG (GE Healthcare, NA931VS; 1/10000) and (HRP)-coupled goat anti-rabbit IgG (GE Healthcare ...
-
bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
-
bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
-
bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
-
bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed with PBST and incubated with alkaline phosphatase-conjugated secondary antibodies (anti-rabbit IgG and anti-mouse IgG, GE healthcare life sciences, 1:10000) for 1 hr at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated for 90 minutes at room temperature with secondary antibodies (donkey anti-rabbit HRP-IgG, GE Healthacare, cat#NA934V, 1:5000 or goat anti-mouse HRP-IgG, GE Healthcare, cat#NA931V, 1:2000) diluted in blocking solution and washed as before ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
-
bioRxiv - Cell Biology 2022Quote: ... followed by anti-rabbit-IgG and mouse-IgG antibodies coupled to HRP (1:2000, NA934, NA931, GE Healthcare).
-
bioRxiv - Cancer Biology 2020Quote: ... The membranes were then incubated with anti-Mouse HRP-conjugated secondary antibody (1:5000 of NA931, GE Healthcare) for 1 hour at room temperature followed by three washes with PBST ...
-
bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies used in Western blotting and IF were HRP sheep anti-mouse IgG (GE Life Sciences NA931V), goat anti-mouse IgG IRDye800CW (LiCoR 926-32210) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... or HRP-conjugated anti-mouse IgG secondary antibody (GE Healthcare, diluted 1:4000 in PBS with 1% BSA). Cells were then incubated in o-phenylenediamine (OPD ...
-
bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
-
bioRxiv - Microbiology 2023Quote: ... were used in combination with secondary anti-mouse antibodies conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). For visualization ...
-
bioRxiv - Physiology 2023Quote: ... and subsequently probed with secondary anti-mouse/-rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... reacted with 5 to 20-fold excess of Alexa-568 or Alexa-488 NHS ester (catalog # A20003, A20000, GE Healthcare) for 1 hour at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931, GE Healthcare UK Limited, UK) in the blocking buffer for 3 h at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-mouse antibody (sheep polyclonal, used at 1:3000, GE Healthcare), HRP-conjugated anti-rabbit antibody (sheep polyclonal ...
-
bioRxiv - Microbiology 2022Quote: ... the plates were incubated with horseradish peroxidase-conjugated sheep anti-mouse IgG polyclonal antibody (GE Healthcare, Chicago, IL, USA). Antigen-antibody interactions were detected using o-phenylenediamine solution as the substrate (Nacalai Tesque) ...
-
bioRxiv - Molecular Biology 2023Quote: ... washed in TBST and detected with anti-Mouse IgG-Horseradish peroxidase (GE Healthcare Life Science, Cat# NA931, 1:5000). For streptavidin staining ...
-
bioRxiv - Cancer Biology 2022Quote: ... using HRP-conjugated secondary anti-mouse antibody (2.5:10 000) and Amersham ECL prime detection reagent (GE healthcare lifescience).
-
bioRxiv - Genetics 2023Quote: ... Blots were washed 3 times with 1x TBS-T and incubated with anti-mouse HRP (GE Healthcare Cat # NXA931) or anti-rabbit HRP secondary antibody (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used were peroxidase-linked anti-mouse IgG and peroxidase-linked anti-rabbit IgG (both Cytiva, formerly known as GE Healthcare Bio-Sciences, NA931 and NA934; 1:5000), Alexa Fluor 594-conjugated donkey anti-mouse IgG (1:250 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
-
bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
-
bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
-
bioRxiv - Cell Biology 2020Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
-
bioRxiv - Neuroscience 2019Quote: ... and HRP-labelled anti-mouse secondary antibody before bands being detected using an enhanced chemiluminescence kit (GE Healthcare, Buckingham, UK). β-actin (Sigma ...
-
bioRxiv - Cell Biology 2019Quote: ... Membranes were quickly washed 3x in TBST (TBS and 0.1% Tween 20) and then incubated with HRPO-linked anti-mouse IgG (both GE Healthcare) diluted 1:3,000 in blocking solution for 45 min ...
-
bioRxiv - Cell Biology 2019Quote: ... The detection of tubulin using mouse anti-tubulin KMX-1 primary (1:100; Gift from Keith Gull, Oxford) and anti-mouse-HRP secondary (1:20,000, GE Healthcare) was used to verify equal sample loading ...
-
bioRxiv - Microbiology 2020Quote: ... The detected proteins were revealed with 1:3,000-diluted horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (GE Healthcare) using a Pierce ECL chemiluminescence substrate (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... After washing the membranes four times for 5 min at room temperature in Washing buffer (PBS, 0.1% (w/v) Tween-20) appropriate HRP-coupled secondary antibodies (anti-mouse IgG (NA931, GE Healthcare), anti-rabbit IgG (NA934 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... in TBS before a 1 hour incubation with a 1:5,000 dilution of sheep anti-mouse IgG horseradish perodixase secondary antibody (GE Healthcare) in 5% milk-TBS ...
-
bioRxiv - Cell Biology 2019Quote: ... we coupled 130 μg of a mouse monoclonal anti-H4K12Ac (gift from Hiroshi Kimura) to 12.5 μl rProtein A Sepharose (GE Healthcare) slurry in antibody coupling buffer (10 mM K-HEPES pH=8 ...
-
bioRxiv - Biophysics 2020Quote: ... followed by washing and incubation with the secondary antibody anti-mouse IgG linked to HRP (1:1000, #NA931, GE Healthcare).
-
bioRxiv - Cell Biology 2019Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
-
bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with specific peroxidase-conjugated secondary antibodies [sheep anti-mouse IgG HRP-linked (1:1000; NA931, GE Healthcare) and donkey anti-rabbit IgG HRP-linked (1:2000 ...
-
bioRxiv - Biochemistry 2024Quote: ... The washing step was followed by 30 min incubation with HRP-conjugated Mouse Anti-M13 monoclonal antibody (GE Healthcare, 27942101) diluted in PBST in 1:5000 ratio ...
-
bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat AntiRat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1 hr at 24 °C with agitation ...
-
bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
-
bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were then washed twice with PBS and incubated for 1 h with anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
-
bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and incubated in 50 μL per well of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) or goat anti-hamster IgG-HRP conjugated antibody (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...