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Citations for GE Life Sciences :
551 - 600 of 1158 citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Human PBMCs were isolated from the blood by density gradient using Percoll (GE Healthcare, 17-5445-01). PBMCs were washed and resuspended in RPMI 1640 supplemented with 10% FBS.
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors using Ficoll-Paque PLUS (GE Healthcare). CD4 T cells were purified from PBMCs using anti-human CD4+ magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare, Cat#17-5442-03). B cells were purified by negative selection (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were obtained from buffy coat by density gradient centrifugation in Ficoll–Paque PLUS (GE Healthcare). PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2019Quote: ... Anti-phospho hTERT rabbit pAbs (anti-249T-P) and anti-rabbit IgG HRP (GE Healthcare) or anti-phospho hTERT mouse mAb (TpMab-1 ...
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bioRxiv - Biochemistry 2020Quote: ... Secondary anti-TOP and anti-actin antibodies (GE Healthcare) were used at 1:10,000 ...
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bioRxiv - Biochemistry 2022Quote: ... mouse VPS35 and VPS29 were placed in vector pGEX4T2 (GE Healthcare), while VPS26 was placed in in-house vector pMWKan ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... or Mouse IgG HRP Linked Whole Ab (GE Healthcare, GENA931-1ML) diluted to 1:10,000 in blocking buffer and incubated at RT for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... or mouse IgG HRP conjugated (GE Healthcare life sciences, Malborough, MA). All primary antibodies were diluted 1:1000 in TBST with 5% BSA from the commercially provided solution ...
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bioRxiv - Molecular Biology 2022Quote: ... secondary α-mouse-HRP conjugated antibody (GE Healthcare, Life Sciences NA931) was used ...
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bioRxiv - Neuroscience 2023Quote: ... and Accell mouse Rbfox1 siRNA (A-041929-13, GE Healthcare Dharmacon). The efficacy of knockdown was validated in previous studies (19 ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 µL of 8.1 µM human Notch1fe were loaded on a Superose6 10/300 increase column (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Cancer Biology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh buffy coats using Ficoll-Paque Plus (GE Healthcare) gradient centrifugation ...
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bioRxiv - Immunology 2022Quote: Human PBMCs were isolated by Ficoll gradient purification as per manufactures protocol (Ficoll, GE-17-1440, GE Healthcare) from human peripheral blood leukapheresis pack (StemCell) ...
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bioRxiv - Neuroscience 2020Quote: ... monkey (XP_005545315.1; 121-760aa) and human (NP_001121620.1; 120-760aa) TfR1 in 0.1% BSA in HBS-EP+ buffer (GE Healthcare) was captured at flow rate 10 μl/min ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated from human CMV-seropositive buffy coats by density centrifugation over Ficoll Paque Plus (GE Healthcare) and screened for the presence of NKG2C+ NK cell expansions by flow cytometry 51 ...
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA encoding human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare, and inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Cell Biology 2024Quote: The human PCM1 C-terminal fragment was cloned into pGEX-2TK GST fusion vector (GE Healthcare Life Sciences) using the primers ...
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bioRxiv - Biochemistry 2021Quote: ... anti-rat IgG and anti-rabbit IgG antibodies (GE Healthcare). Blots were detected using ECL Prime Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by Ficoll-Hypaque density gradient centrifugation (GE healthcare). Monocytes were isolated from PBMCs using magnetic CD14+ isolation beads (Miltenyi) ...
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bioRxiv - Immunology 2019Quote: PBMCs were isolated from whole human peripheral blood by Ficoll-Paque PLUS (17-1440-03, GE Healthcare, Pittsburgh, PA) density gradient centrifugation and cryopreserved prior to use ...
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bioRxiv - Cancer Biology 2019Quote: STAT3 siRNA knockdown utilized the ON-TARGETplus Human STAT3 siRNA kit from GEHealthcare (L-003544-00-0005, GE Healthcare). This SMARTpool siRNA contains four pooled siRNAs ...
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bioRxiv - Genetics 2019Quote: ... Pools of siRNA against human NOVA2 gene and of Scramble siRNA were purchased from Dharmacon (GE Healthcare, Lafayette, CO).
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bioRxiv - Biochemistry 2022Quote: ... The complementary DNA of human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare. The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Cell Biology 2023Quote: ... Fibronectin was isolated from human plasma using Gelatin-Sepharose 4B (Cat No. 17-0956-01, GE Healthcare, Sydney, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats using Ficoll-Paque PLUS (GE Healthcare Bioscience). The PBMCs were cryopreserved in 40% heat-inactivated fetal bovine serum (FCS ...
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bioRxiv - Biochemistry 2024Quote: The complementary DNA of human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare. The hZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Cell Biology 2020Quote: ... ECL Secondary Mouse and Rabbit HRP antibodies used were from GE Healthcare.
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bioRxiv - Molecular Biology 2019Quote: ... and mouse IgG HRP linked F(ab’)2 fragment (NA9310, GE Healthcare). Luminescence was detected with a luminescent image analyser (Amersham Imager 600 ...
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (mouse Aldh1a3, Clone ID 6515355, purchased from GE Healthcare) were inserted into pCAGIG vector (pCAGIG was obtained from Addgene (Plasmid # 11159).
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bioRxiv - Microbiology 2020Quote: ... and secondary antibodies α-mouse IgG (H&L)-HRP (GE healthcare #NXA931), 1:5000 and α-rabbit IgG (H&L)-HRP (GE Healthcare #NA934) ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated mouse and rabbit secondary antibodies were obtained from GE Healthcare. FemtoLUCENT™ PLUS HRP Kit (786-10 ...
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bioRxiv - Pathology 2021Quote: ... cynomolgus or mouse LRG1 were determined by SPR (Biacore T200, GE Healthcare). All samples were buffer exchanged into running buffer HBS-EP+ ...
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bioRxiv - Genetics 2021Quote: ... anti-rat or anti-rabbit HRP-conjugated antibodies (GE Life Sciences) as appropriate ...
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bioRxiv - Microbiology 2019Quote: ... anti-rabbit or anti-goat as the secondary antibody (GE Healthcare) were used at a dilution of 1:3000.
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bioRxiv - Microbiology 2019Quote: ... Virus labeling was performed with Alexa Fluor™A647 succinimidyl ester in a 1:10 molar ratio and was then purified through Nap-5 desalting column (GE Healthcare). Labeled viruses were aliquoted and stored at −80°C ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μg of protein per sample were solved in urea lysis buffer and labeled with 250 pmol CyDye DIGE Fluor dyes (GE Healthcare, Germany). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... excess unbound Alexa 555 was removed using a HiTrap® Desalting Column connected to an Äkta purifier system (GE Healthcare). Finally ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were isolated from healthy volunteer donors by layering over Ficoll-Paque Plus (GE Healthcare) as per the manufacturer’s instructions and were enriched for CD4+ or CD8+ T cells using RosetteSep (STEMCELL Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Microbiology 2020Quote: Human PBMCs were isolated from leukapheresis obtained from the Central Blood Bank (Pittsburgh, PA) using Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... Human IgGs were isolated from heat-inactivated plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) as described (24).
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bioRxiv - Cell Biology 2022Quote: Fibronectin was purified from outdated human plasma (American Red Cross, Rochester, NY) using gelatin-Sepharose (GE Life Sciences, now Cytiva) affinity chromatography (102) ...
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bioRxiv - Microbiology 2021Quote: ... A549 and human colon carcinoma LoVo cells (both purchased from ATCC) were cultured in F12 Kaighns Modification media (GE Healthcare) with 10% FBS and 1% penicillin/streptomycin.
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bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells were isolated from fresh whole blood by Ficoll-Paque (GE Healthcare, Little Chalfont, United Kingdom). CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... Human MSLN (Advance BioMatrix 5123-0.1mg) was (10 mg/mL) was immobilized onto a CM5 sensor chip (GE Healthcare, BR100012) by amine coupling ...