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Citations for GE Life Sciences :
401 - 450 of 5411 citations for Adenovirus Type 5 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... The GST-tagged proteins were expressed and purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) following previously published protocols (42) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Cancer Biology 2022Quote: ... All protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare). Further details can be found in our previous publication [14].
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were clarified followed by protein A affinity chromatography with MabSelect SuRe LX protein A resin (GE Healthcare) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by on-column digestion and the eluate was further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: GST-fused bacterial expression plasmid for PAX9 was created by subcloning wild type and mutant (c.336C>G) PAX9 in BamHI and SalI site of pGEX4T1 vector (GE Healthcare, USA). E ...
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bioRxiv - Cell Biology 2019Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30 min at 37°C with shaking at 100 rpm ...
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bioRxiv - Immunology 2022Quote: ... a Histrap excel 5 mL column (GE Healthcare) was used to isolate proteins ...
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bioRxiv - Plant Biology 2019Quote: ... pH 7.9 through NAP-5 column (GE Healthcare) and brought to the desired concentration either by dilution or by concentration through Amicon-Ultra device (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... a HisTrap HP 5 ml column (GE Healthcare) was used ...
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bioRxiv - Developmental Biology 2019Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... a MonoQ 5/50 GL column (GE Healthcare) was required to completely remove the GST-tag ...
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bioRxiv - Biochemistry 2020Quote: ... using a 5 mL HisTrap column (GE Healthcare). The bound protein was eluted using a linear imidazole gradient (0 - 0.5 M ...
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bioRxiv - Biophysics 2021Quote: ... while a Tricorn 5/20 column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... a 5 ml HisTrap column (GE Healthcare, UK) was used for immobilized metal affinity chromatography (IMAC) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented 5% horse serum (GE Healthcare Life Sciences), 1% penicillin/streptomycin (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: ... using a 5 mL StrepTrap HP (GE Healthcare). The washing buffer contained 100 mM Tris pH 8.0 and 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 5 ml HisTrap™ HP column (GE Healthcare). For further purification steps the column was connected to a liquid chromatography system (Azura ...
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bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% membrane blocking agent (GE Healthcare) in TBS and incubated with the corresponding primary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... IMAC Column: 5 ml HisTrap HP (GE Healthcare); Gel Filtration Column ...
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bioRxiv - Molecular Biology 2022Quote: ... loaded onto 5 ml HisTrap column (GE Healthcare), equilibrated in buffer A ...
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bioRxiv - Microbiology 2019Quote: ... or 5 ml GSTrap HP column (GE Healthcare), depending on the fused tag ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 45 min at 37°C with shaking ...
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bioRxiv - Microbiology 2021Quote: ... A 5 ml HiTrap desalting column (GE Healthcare) was used to exchange the complex buffer into 100 mM ammonium acetate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... supplemented with 5% fetal bovine serum (GE Healthcare) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Amersham 5 AI600 (GE Healthcare, USA).
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bioRxiv - Microbiology 2020Quote: ... proteins were immobilized to a protein A sensorchip a level of ~500 response units (RUs) using Biacore 8000 (GE Healthcare) and a running buffer of composed of 20mM PB7.4 with 300 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Microbiology 2021Quote: ... The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences). The eluted fractions were collected for SDS-PAGE analysis.
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was further purified by fast protein liquid chromatography (FPLC) using a Source-S cation-exchange column (GE Healthcare) (low salt buffer with 50 mM potassium phosphate at pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were further purified by gel filtration on a fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principle peaks were collected ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was added to 50 µl G-Protein Sepharose beads (Protein G-Sepharose 4 Fast Flow, GE Healthcare) (pre-washed with the lysis buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein G-coupled sepharose (#17–0618–01) and Protein A-coupled sepharose (#17–5138–01) beads were from GE Healthcare and the protease inhibitor cocktail was from Sigma.
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bioRxiv - Microbiology 2020Quote: ... Proteins of interest were detected with protein-specific primary antibodies and HRP-coupled secondary antibodies by enhanced chemiluminescence (GE Healthcare) and imaged using X-ray films or with Fusion Capture Advance FX7 16.15 (Peqlab).
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Biophysics 2021Quote: ... Protein-transferred PVDF membranes (GE Healthcare Life Science) were blocked with 5% skim milk in PBS-T ...
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bioRxiv - Biophysics 2021Quote: ... proteins were visualized using ECL prime (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: M-PER mammalian protein extraction reagent (GE Healthcare) was used to extract protein from mOSE cells and run on NuPAGE 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Developmental Biology 2021Quote: ... antibodies and protein G Mag sepharose (GE Healthcare) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein transfer onto nitrocellulose membranes (GE Life Sciences) was carried out for 90 min at 25 W in a Mini Trans-Blot Electrophoretic Transfer Cell (BioRad) ...
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bioRxiv - Biochemistry 2019Quote: ... 45 μl Protein G Sepharose beads (GE Healthcare) were added ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein G Sepharose beads CL-4B (GE Healthcare) were pre-equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were exposed with ECL prime (GE Healthcare) at a ChemiDox XRS imaging system (Bio-Rad).Primary antibodies were used against H3K27me3 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to nitrocellulose membrane (GE Healthcare) and detection was performed using anti-His primary antibody (Penta-His Antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Fast-flow Protein-A sepharose beads (GE Healthcare) (250 µl slurry ...
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bioRxiv - Plant Biology 2020Quote: ... 50 □L of Protein G Sepharose (GE Healthcare) was used in accordance with the manufacturer’s instructions ...