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Citations for GE Life Sciences :
501 - 550 of 5411 citations for Adenovirus Type 5 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the reconstituted MBP-CENP-C1-600-Spy-CENP-C601-943-8xHis protein was separated from unligated protein fragments on a Superose 6 10/300 size-exclusion column (GE Healthcare) as described above ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Immunoglobulins were purified against Protein A from 1 ml of rabbit serum using a Protein A HP SpinTrap (28-9031-32, GE Healthcare) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: Affinities and kinetic parameters of protein-protein interactions were measured by surface plasmon resonance (SPR) analysis using a Biacore T100 biosensor (GE Healthcare). 2000 response units (RU ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... Kinetic parameters and affinities of protein-protein interactions were measured by surface plasmon resonance (SPR) analysis using a Biacore T100 biosensor (GE Healthcare). 2000 response units (RU ...
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bioRxiv - Microbiology 2021Quote: ... Protein size was analyzed using a calibration curve of proteins included in the high molecular weight calibration kit (GE Healthcare, Chicago). To test frozen samples for aggregation ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting protein/phospholipid complexes were separated from the remaining vesicles and soluble protein by SEC using a Superose 6 column (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved protein was further purified with size exclusion chromatography to remove any protein aggregates applied to a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2021Quote: ... The GST tag was cleaved from purified protein by elution of the tag-free protein from the column by overnight incubation with PreScission Protease (GE Healthcare) or alternatively by elution with 15 mM glutathione-containing purification buffer ...
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bioRxiv - Biochemistry 2020Quote: ... For X-ray crystallography the protein was further purified using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare) over a HiPrep 16/60 Sephacryl S-100 high-resolution gel filtration column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing the protein of interest were pooled and concentrated to ∼3 mg/mL using a 100kDa MWCO Vivaspin protein concentrator (GE Healthcare). The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by either Protein A affinity or by using ion exchange chromatography (MonoQ 10/100 GL column, GE Healthcare) with a 0 mM–1 M NaCl gradient ...
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and a two-step fast protein liquid chromatography (FPLC) purification protocol using an ÄKTA Protein Purification System (GE Healthcare Life Sciences) at 8 °C was then followed to obtain the recombinant protein ...
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bioRxiv - Immunology 2022Quote: ... The culture was harvested after seven days and the supernatant purified into PBS by Protein G affinity using a HiTrap Protein G HP affinity column (GE Healthcare), eluted with 0.1M acetic acid (VWR international ...
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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bioRxiv - Microbiology 2021Quote: ... Expression of the recombinant proteins was performed using the ExpiCHO Expression system (Thermoscientific) following purification using HiTrap Protein-A column (GE Healthcare). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Biophysics 2019Quote: ... After dilution of the protein sample to a DTT concentration of 1 mM the reduced protein were immobilized on a Ni2+-Sepharose resin (GE Healthcare) and washed with ten column volumes of buffer A (50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were centrifuged at 12,000 x g for 30 min and the supernatant was subsequently used for protein purification through a HisTrap column (His- tagged full-length proteins, GE Healthcare) or Amylose resin (MBP-tagged fragments ...
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bioRxiv - Immunology 2021Quote: ... The ACE2-Ig and RBD-Ig fusion proteins secreted to the medium were purified on HiTrap Protein G High Performance column (Cat#GE17-0405-01, GE Healthcare). Control-Ig was one of the following fusion proteins ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing the untagged protein were pooled and the homogenous protein was finally isolated by size-exclusion chromatography onto a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Hepes/NaOH ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed using BCA and proteins was separated on SDS-PAGE followed by transfer to PVDF membrane (GE Healthcare). Next ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
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bioRxiv - Immunology 2021Quote: The affinity measurements of hemin towards wild type (hCD36ecto) or mutants (R292A, D372A and Q382A) were performed in MicroCal iTC200 isothermal titration calorimeter (GE Healthcare, Chicago, USA). Unless specified ...
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bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
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bioRxiv - Microbiology 2022Quote: ... was supplemented with 4 mM MgCl2 and 5 mM imidazole and loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 0.1 % LDAO ...
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bioRxiv - Cell Biology 2021Quote: ... 5% fetal bovine serum (FBS; GE Healthcare, Chicago, IL), and 0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were imaged using Typhoon 5 Imager (GE Healthcare) to record signals from the Cy5 dye ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare) was used for further purification which resulted in a monodisperse and clear INO80 complex ...
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bioRxiv - Biophysics 2019Quote: ... loaded onto a 5-mL HisTrap column (GE Healthcare) and finally eluted with 220 mM imidazole in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... on a MonoQ 5/50 GL column (GE Healthcare). Sample purification and analysis with HPLC were performed using Agilent 1260 Infinity (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl of streptavidin beads (GE Healthcare, Chicago, USA) per O.D.254 unit was added ...
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bioRxiv - Bioengineering 2021Quote: ... a Sephadex G-25 column (GE Healthcare, NAP-5) was equilibrated with PBS (PH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... A HisTrap HP column (5 ml, GE Healthcare, France), prepacked with Ni High Performance Sepharose ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were scanned using a Typhoon 5 (GE Healthcare) and the signal intensity quantified using Image Studio Lite (LI-COR Biosciences).
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bioRxiv - Biochemistry 2022Quote: ... applied to a 5 ml HisTrap HP (GE Healthcare), and washed with lysis buffer using an Äkta pure fast-performance liquid chromatography system (GE Healthcare,) ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare, Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... using a 5 mL HisTrap FF column (GE Healthcare). Bacterial pellets were lysed in a buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... using a SD200 Increase 5/150 column (GE Healthcare) at 0.4 ml/min with 1 frame recorded per second (Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... first with HisTrap HP 5 mL columns (GE Healthcare) and next with a size-exclusion HiLoad Superdex 200 PG column (GE healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting sample was applied to a 5 ml HiTrap HP heparin column (GE Healthcare) equilibrated with TGE containing 50 mM NaCl and 1 mM β-mercaptoethanol ...