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Citations for GE Life Sciences :
4351 - 4376 of 4376 citations for Recombinant Bovine CTLA4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were first purified with affinity chromatography using AKTA pure 25 system and HiTrap MabSelect SuRe protein A column (GE healthcare, Cat# 11-0034-94) for huACE2-IgHu-pVax ...
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bioRxiv - Biochemistry 2022Quote: Verification of the monomeric or dimeric state of those constructs was obtained by SEC-MALS experiments with the injection of 5 mg/mL of each protein into a Superdex 200 10/30 Increase column (GE Life Sciences, Velizy-Villacoublay, France) mounted on a HPLC Ultimate 3000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with developing solution kit (Amersham™ ECL Select™ Western Blotting Detection Reagent) and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were injected and purified on SEC (size exclusion chromatography) column Superdex 200 10/300 GL (GE Healthcare Life Sciences, Piscataway, NJ, USA) against a buffer containing 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: 1 mg lysate protein aliquots were used for immunoprecipitation with optimized amounts of the indicated antibodies and 20 μL of Protein A Sepharose beads (GE Healthcare, Chalfont St. Giles, UK) followed by SDS-Polyacrylamide (Biorad ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was further purified by size exclusion chromatography using HiLoad® 16/600 Superdex® 75 pg column (GE Life Sciences, 28-9893-33) equilibrated with 1x Tris buffer (50 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... The protein samples were then applied to size exclusion chromatography (Superdex 200 prep grad, HiLoad 26/60 or 16/60, GE Healthcare Life Sciences, Freiburg, Germany) using 50 mM HEPES-KOH (pH 7.2) ...
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bioRxiv - Plant Biology 2024Quote: ... His-tagged recombinant proteins were overexpressed in Escherichia coli strain Rosetta™ (DE3) and purified via an Äkta chromatography system using HisTrap columns (GE Healthcare, Waukesha, WI, USA). Washing (20 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... the oligomers were separated from the monomeric and fibrillar forms of the protein by size exclusion chromatography on a Superose 6 HR10/300 column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA) equilibrated in PBS pH 7.4 at a flow rate of 0.5 mL/min ...
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Biochemistry 2021Quote: Size exclusion chromatography (SEC) of the concentrated protein solution was conducted using a HiLoad™ 26/600 Superdex™ 200 pg column (GE Healthcare, Mu-nich, Germany) coupled to the aforementioned chromatography system ...
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bioRxiv - Cancer Biology 2019Quote: ... The immune complexes were mixed with 20 μl of Protein A or G sepharose 4 Fast flow beads (50% v/v, GE Healthcare Life Sciences, Marlborough, MA, USA). Whole cell lysates and immunoprecipitates were detected by Western blotting ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purest form of the proteins were loaded on a size exclusion chromatography HiLoad® 16/60 Superdex® 200 pg column (GE Healthcare Life Sciences / Cytiva), pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... Separation of aSYN monomers and PFFs was performed with fast protein liquid chromatography using a Superose 6 10/300GL column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). PFFs were briefly sonicated ...
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bioRxiv - Biophysics 2020Quote: Proteins were finally separated from free fluorophores by size exclusion chromatography with a PD MiniTrap G-25 prepacked columns (GE Healthcare Life Science, Chalfont St Giles, UK). Finally ...
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bioRxiv - Molecular Biology 2021Quote: ... The EGFP/YFP tags on the proteins was detected by applying SuperSignal® West Dura Extended Duration Chemiluminescent Substrate and imaging with an Amersham Imager 600RGB (GE Healthcare Life Sciences, Little Chalfont, UK).
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bioRxiv - Cell Biology 2024Quote: Cell or collagen lysates were separated by denaturing SDS-PAGE (6% gels for collagens, 10% for SMADs) and proteins blotted onto a nitrocellulose membrane (Amersham™, GE Healthcare, Chalfont St Giles, United Kingdom). Membranes were cut horizontally to detect proteins of different sizes ...
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bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...
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bioRxiv - Biophysics 2022Quote: ... with salt gradient of (250-1000mM NaCl for PARP1 and 50mM-1000mM NaCl for all other constructs) and finally through gel filtration (16/600 200pg or 16/600 75pg, GE Healthcare, depending on the size of the protein) column pre-equilibrated with buffer 150mM NaCl ...
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bioRxiv - Microbiology 2022Quote: S309-LS and S309-GRLR were produced in ExpiCHO-S cells and affinity-purified using HiTrap Protein A columns (GE Healthcare, HiTrap mAb select Xtra #28-4082-61) followed by buffer exchange to histidine buffer (20 mM histidine ...
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bioRxiv - Molecular Biology 2020Quote: ... Soluble chromatin was pre-cleared by incubation with 10 μL of 50% RIPA-equilibrated Protein A + G sepharose bead slurry (GE Healthcare, Cat. No 17-5280-11 and 17-0618-06) for every 100 μL of chromatin for 1 h at 4°C ...