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Citations for GE Life Sciences :
4051 - 4100 of 4376 citations for Recombinant Bovine CTLA4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The denatured proteins were resolved on 8–12 polyacrylamide-SDS gels and transferred onto PVDF membrane (GE Healthcare, Chicago, IL, USA). A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color ...
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bioRxiv - Microbiology 2024Quote: ... The protein was further purified using size exclusion chromatography with a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare) into a final buffer of 20 mM HEPES pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was separated on an 8-16% gradient Tris-glycine gels and transferred to PVDF blotting membrane (GE Healthcare Life Sciences). The membrane was immunoblotted with 1:200 anti-FGF13 antibody or 1:200 anti-pan FHF-A (Neuromab clone N235/22 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein detection and quantification were performed using the SYBR-green fluorescence detection mode of the LAS-4000 image analyzer (GE Healthcare) and Image Quant TL software (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were detected with host-specific secondary antibodies and developed with a chemiluminescent substrate (GE Healthcare Amersham, Piscataway, NJ, USA). After stripping ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Biophysics 2024Quote: ... monomeric and tetrameric protein were separated and collected by SEC using a Superdex 75 Increase 10/300 GL column (GE Healthcare) and then used directly in fluorescence experiments (SI Appendix ...
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bioRxiv - Biophysics 2024Quote: ... TEV-cleaved protein was then diluted 1:500 in KBT and then loaded onto a HiTrap Q HP column (GE Healthcare) for ion exchange chromatography (IEC ...
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bioRxiv - Plant Biology 2024Quote: ... His6-tagged proteins (His6-HsfA2/S-HsfA2/S-HsfA4c/S-HsfB1/S-HsfB2a/DBD/tDBD) and His6 mock proteins were purified using glutathione Sepharose 4B beads (GE Healthcare) and nickel-nitrotriacetic acid (Ni-NTA ...
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bioRxiv - Microbiology 2024Quote: ... Full-length untagged cGAS protein was then ultimately purified using size-exclusion chromatography via a 16/600 Superdex S75 column (GE Healthcare). Purified cGAS was concentrated to ∼10 mg/ml in storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing L–P protein were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2024Quote: ... The molecular weight of protein complexes was determined by size exclusion chromatography performed on Superdex 200 Increase 10/300 column (GE Healthcare) calibrated with High Molecular Weight calibration kit (GE Healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, PA) using AKTA pure FPLC system ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein separation was carried out with different ion exchange columns: A cation exchange column (HiScreen Capto SP ImpRes, GE Healthcare, München, Germany), an anion exchange column (HiTrap Q HP 1 ml ...
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bioRxiv - Biophysics 2019Quote: ... The protein bands were subsequently imaged using an Amersham Imager 600 instrument and its accompanying software (v0.9.8) (GE Healthcare, Chicago, IL, USA) with a 1 s exposure time ...
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... 150 μL GIT1-CC or β-Pix-CC protein sample at 50 μM was loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... Cleaved protein was separated from TEV and the His-GST tag with a Ni-NTA column and proteins were further purified by size-exclusion chromatography on Superdex 75 16/600 (GE Life Sciences) in NMR buffer (10 mM MES ...
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bioRxiv - Neuroscience 2021Quote: ... Co-IPs done to ckeck for homodimers were done the same way except that agarose beads (Protein G Sepharose™ 4 Fast Flow by GE Healthcare) were used that were incubated with mouse anti-myc antibody (9B11 ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Microbiology 2019Quote: ... Affinity-purified proteins were polished by size-exclusion chromatography (SEC) on Superdex 75 or Superdex 200 columns (GE Healthcare, Chicago, IL, USA) equilibrated with buffer B2 (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: Co-immunoprecipitations of Rps3p with Bfr1p-3HA were performed with similar conditions to Bfr1p-yeGFP but immunoprecipitated with Protein G Sepharose® 4 Fast Flow beads (GE Healthcare). Prior to the immunoprecipitations ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences) column equilibrated in Buffer B (50 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... The LytH protein was further purified using size-exclusion chromatography with a Superdex 200 Increase 10/300 GL column (GE Life Sciences) equilibrated in Buffer C (25 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... The unbound protein fraction containing biotin-PS-CFP2 was again purified via gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences). The monomeric biotin-PS-CFP2 was stored in 1 x PBS at −80 °C.
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bioRxiv - Biophysics 2019Quote: GST-fusion proteins (UBAN domains of OPTN, ABIN, NEMO and Ub) were cloned into pGEX-4T-1 (GE Life Sciences, Freiburg, Germany) and site-directed mutagenesis was performed by PCR to introduce desired amino acid substitutions.
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bioRxiv - Microbiology 2020Quote: ... The labeled protein was separated from the excess of free 125I in a PD10 desalting column (GE Healthcare Life Sciences, United Kingdom) and the purity of the 125I-labeled Vip3Aa was checked by autoradiography ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted Cavin4a-FL and Cavin4b-FL proteins were loaded on size exclusion chromatography column Superose™ 6 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 0.22 μm filtered 50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Yield can be improved by taking pooled TRAPP complex before SEC and manually running protein through a 1 mL HisTrap™ FF column (GE Healthcare) that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... TEV protease-digested proteins were passed over Ni-NTA agarose to remove uncleaved proteins and then further purified by size exclusion chromatography using a Superdex200 column (GE Life Sciences) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed once with PBS containing 0.05% Tween-20 (PBST) and then incubated with Protein A/G Sera-Mag SpeedBeads (GE Life Sciences, 17152104010350) for 1 hour at 4°C with orbital shaking ...
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bioRxiv - Biochemistry 2021Quote: Samples were dialysed overnight in the corresponding buffers and different concentrations of protein were loaded onto a size-exclusion Superdex200 Increase 3.2/300 column (GE Healthcare Life Science) at a flow rate of 50 μL/min ...
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bioRxiv - Bioengineering 2021Quote: ... The eluted protein was then buffer exchanged to 10 mM HEPES at pH 7.4 by PD-10 columns (GE Healthcare Life Sciences) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... CALM ANTH domain and Ub proteins were further purified on a HiLoad 16/60 Superdex 200 column (GE Healthcare Biosciences, Pittsburg, PA) equilibrated with 20mM HEPES-NaOH ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed using an M-110S Microfluidizer (Microfluidics) and proteins were purified using glutathione chromatography according to the manufacturer’s instructions (GE Healthcare Life Sciences). To confirm the size and purity of purified recombinant protein ...
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bioRxiv - Plant Biology 2021Quote: ... Oligomeric states of OsbZIP47 protein was determined by analytical size exclusion chromatography (SEC) performed at 4°C on a Superdex 200 increase column (GE Healthcare Inc.) pre-equilibrated with buffer (25 mM sodium phosphate (pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: The IgG fraction from the hyperimmunized rabbit serum was purified on 2x 1 mL HiTrap™ Protein G HP columns (GE Healthcare). And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... The protein was then separated by size-exclusion chromatography (SEC) using a Superdex 75 16/600 column (GE Healthcare Life Sciences, UK) equilibrated with PBS pH 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare, UK) (Nettleship et al. ...
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bioRxiv - Biophysics 2020Quote: ... protein was concentrated and further purified by size exclusion chromatography using a HiLoad® 16/600 Superdex 200 pg column (GE Healthcare) in Dialysis Buffer ...
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bioRxiv - Biophysics 2020Quote: ... For ion exchange chromatography (IEX) the protein was loaded onto a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Sweden) and eluted against a linear gradient of 7 column volumes (CV ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 10 μg of total protein was separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane with either 0.4 μm pores (GE Healthcare, Chicago, IL) or 0.2 μm pores (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Biophysics 2020Quote: ... Unconjugated TFP ester dyes were separated from labeled proteins using HiTrap Desalting columns with Sephadex G-25 resin (GE Healthcare Life Sciences). Alexa Fluor™ 647 C2 Maleimide (A20347 ...
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bioRxiv - Molecular Biology 2020Quote: ... Flow rate was 0.75 ml/min and protein elution was monitored with the U9-L UV monitor (GE Healthcare, Chicago, IL, USA) at 280 nm.
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bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated protein was further purified using size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare, Chicago, IL, USA) column containing 0.003% (w/v ...