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Citations for GE Life Sciences :
4201 - 4250 of 4565 citations for Recombinant Human CD19 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using SDS-PAGE in 10 % Bis-Tris acrylamide/ bisacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). The membranes were blocked for 30 minutes with PBS 5 % milk and incubated with the first antibody for 1 hour at room temperature or at 4°C over-night with gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... The protein elutes in the flow through and is subsequently subjected to size exclusion chromatography using a Superdex 200 (GE Healthcare) in a buffer containing 50 mM HEPES (pH 8.2) ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein of interest was then purified by size exclusion chromatography using a Superdex 75 prep grade column (GE Healthcare). Where necessary ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP tag was cleaved off by TEV protease and the protein was further purified by a passage through a Ni2+-Chelating Sepharose (GE Healthcare). IHO1 was then purified by a gel filtration on Superdex 200 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biophysics 2023Quote: Ion exchange chromatography (IEC) was performed using an ÄKTA Purifier protein purification system controlled by the software Unicorn 3.10 (GE Healthcare, Sweden). CIMmultus QA 1 mL column (BIA Separations ...
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bioRxiv - Biophysics 2023Quote: ... The protein eluate was concentrated and further purified by size-exclusion chromatography on a Superose6 10/300 GL column (GE Healthcare). Peak fractions were collected for functional assay.
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bioRxiv - Immunology 2022Quote: ... Samples were resolved on 12–15% SDS–PAGE gels and then proteins were wet transferred to Protran nitrocellulose membranes (GE Healthcare) at 400 mA at 4 °C for 1.5 h in 1x Transfer buffer (25 mM Tris base ...
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bioRxiv - Cell Biology 2022Quote: Cellular lysates were separated on a Superose 6 HR 10/30 size-exclusion column and an AKTA Purifier protein purification system (GE Healthcare), essentially as described previously(61) ...
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bioRxiv - Cell Biology 2022Quote: ... The tag-free protein was further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare). Mutants and ZF truncations were expressed and purified using the same procedure as for the wild-type proteins ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was concentrated and subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare, 289909944) that was pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The biobeads were removed by filtration and the protein-amphipol mixture was concentrated before purification by size exclusion chromatography using a Superdex S200 column (GE Healthcare) pre-equilibrated with buffer SEC2 (200 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then washed in PBS-T and incubated with 1 mL of Enhanced ChemiLuminescence (ECL) (Amersham) reagent for 5 minutes to allow protein visualization on an x-ray Amersham Hyperfilm ECL (GE Healthcare). Fiji-Image J Software was used for quantification of the difference in protein expression comparing with GAPDH or HSP70 expression.
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bioRxiv - Biophysics 2023Quote: ... The concentrated protein sample was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL (GE Healthcare) column pre-equilibrated with the buffer E (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: GST fusion protein was similarly purified using affinity chromatography by incubating bacterial cell lysate with glutathione-Sepharose 4B beads (#17075601; GE Healthcare) according to the manufacturer’s instructions and eluted from beads with elution buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... TCA extracts of total yeast or 2 μg of mitochondrial proteins were loaded on a 12 % SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare). UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD) ...
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bioRxiv - Immunology 2023Quote: ... Protein-containing fractions were combined and purified further via SEC on a Superdex 75 10/300 GL column (GE Healthcare/Cytiva) using 20 mM sodium phosphate buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... was used as the secondary antibody. Protein was detected using the chemiluminescent substrate ECL (Cat. # 32209, Pierce) in an Amersham Imager 680 (GE Healthcare) detector.
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Biophysics 2023Quote: ... the protein was subjected to ion exchange chromatography and loaded onto a HiLoad 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl with a salt gradient from 0 M to 1.5 M NaCl in buffers containing 25 mM Tris-HCl (pH 7.7) ...
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bioRxiv - Biochemistry 2023Quote: ... After His-tag purification protein was subsequently purified by size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare). The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by gel filtration using fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superdex 200 Increase 10/300 column (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was again washed three times with TBST and the protein of interest was detected using a chemiluminescence reagent (ECL Prime, GE Healthcare). Chemiluminescence was detected using the ImageQuant LAS500 imager (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... insoluble material was removed by centrifugation at 10,000 × g and the cell lysate was pre- cleared by addition of heat inactivated goat serum and protein G sepharose beads (GE Healthcare). Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1 ...
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bioRxiv - Cell Biology 2023Quote: ... Reduced immunoprecipitated proteins were separated on a 10 or 12% SDS-polyacrylamide gel and transferred to a PVDF membrane (GE Healthcare). Details on the blocking of the membranes and detection of the proteins can be found in Table S1 ...
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bioRxiv - Immunology 2022Quote: ... Proteins in gels were either stained with Coomassie blue dye in gel or used for transfer onto nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in PBST (PBS and 0.05% Tween-20 ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were separated by 15% SDS-PAGE and transferred to 0.2 µm Nitrocellulose Blotting Membrane (#10600001, GE Healthcare Life Sciences) by the semi-dry method (Towbin et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010; or Amersham Hybond, GE Healthcare Life Science, 10600023), and immunodetected using primary antibodies directed against HSP60 rabbit polyclonal (D307 ...
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bioRxiv - Neuroscience 2023Quote: ... 3RCF and 4RCF tau proteins were further purified by FPLC using size exclusion Superdex-75 and Superdex-200 columns (GE Healthcare) in 1 x PNE buffer (25 mM PIPES ...
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bioRxiv - Neuroscience 2023Quote: Purified His-tagged DJ-1 and untagged PGK1 proteins were subjected to further purification using gel filtration with a Superdex200 Increase 10/300 GL size-exclusion column (GE Healthcare) on an AKTA FPLC system (Cytiva) ...
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bioRxiv - Plant Biology 2024Quote: ... Co-precipitation of StCWC15a/b with IPI-O1 was examined by protein blotting before (input) and after affinity purification (pull-down) using glutathione agarose beads (GE Healthcare). Interactions were detected using anti-GST and anti-His (Abmart).
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bioRxiv - Molecular Biology 2023Quote: ... 200 μg of total protein extracts were fractionated in a 10 % SDS-PAGE followed by a wet transfer to a PVDF membrane (GE Healthcare) [78] ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant loaded to the column and purification was performed by using ÄKTA start protein purification system (GE Healthcare Life Sciences) according to the preferred protocols provided by the purification column manual ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superose 6 Increase size exclusion chromatography column (10/300 prep grade, GE Healthcare). Elution was carried out with 150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant antibodies were produced in a transient HEK expression system and purified via protein A affinity purification on an automated AKTA system (AKTA explorer FPLC, GE Healthcare). For in vivo studies rabbit V-regions were fused with murine constant of mouse IgG2a heavy chain and kappa chain to minimize immunogenicity ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing the protein of interest were concentrated and further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... 200μl of the protein mixtures were analyzed by size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare) using a GE ÄKTA FPLC™ (Fast Protein Liquid Chromatograph ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was further purified by size exclusion chromatography (SEC) with a HiLoad 26/60 Superdex 75 pg column (GE Healthcare) in SEC buffer (20 mM Sodium-phosphate buffer pH 6.5 ...
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bioRxiv - Biophysics 2024Quote: ... to remove metal ions and polysulfane sulfur before proteins were subjected to anaerobic size exclusion chromatography (SEC) (HiLoad 16/600 Superdex; GE Healthcare) on an Äkta Purifier system (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (AmershamTM Protran® 0.45 µm, GE Healthcare Chicago, IL, USA). Membranes were blocked for 1 hour with 5% BSA in TTBS (15 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...