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Citations for GE Life Sciences :
4101 - 4150 of 4723 citations for Recombinant Human FCGR3B Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove soluble aggregates ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... The affinity-purified protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... but no 3C protease cleavage was performed and the protein was further purified on a Superdex 200 Increase 10/300 GL size-exclusion chromatography column (GE Healthcare) equilibrated in 100 mM NaCl ...
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bioRxiv - Biophysics 2020Quote: ... The Anti-hIgG Fc (AHC) capture biosensors were purchased from ForteBio (California, USA) and the protein A coated chips were purchased from GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... followed by 2 CV of 100% IEX buffer B using an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare). To determine the point of elution of aSyn from the chromatography column protein fractions which were collected and monitored on absorption at 280 nm were ran on a 4-12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... an equal amount of protein sample (between 10-20 μg) was separated by SDS-PAGE and then transferred onto a nitrocellulose membrane (Hybond, GE Healthcare) for two hours at 250 mA on ice ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein eluted with a gradient from 20 to 500 mM imidazole over 10 column volumes using an AKTA Pure (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... After that the labeled protein was purified from the excess of free dye by a PD SpinTrap G-25 column (GE Healthcare), divided into aliquots and stored at -80 °C.
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bioRxiv - Cancer Biology 2022Quote: ... cell supernatants/lysates were precleared using 30 μl of a 50% slurry of Protein G Sepharose (GE Healthcare, #17-0618-01), while for IP of pyo-KSR1 ...
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bioRxiv - Cell Biology 2022Quote: ... After incubation with primary antibodies overnight at 4°C followed by the addition of Protein A or G Sepharose beads (GE Healthcare) for 2-3 hrs ...
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration was adjusted to 250 mM KCl and the protein was loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.
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bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was purified with subtractive Ni-NTA followed by purification on a Superdex 75 16/600 GL column (GE Healthcare), in a size exclusion buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... tagging of the protein was visualised by running an SDS PAGE followed by scanning in a Typhoon scanner (GE Healthcare, Sweden).
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bioRxiv - Biochemistry 2022Quote: All chromatography purification steps were carried out at 4°C using an ÄKTA pure fast protein liquid chromatography (FPLC) system (GE Healthcare). All buffer solutions were chilled prior to use ...
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bioRxiv - Molecular Biology 2022Quote: ... immobilized protein was eluted using wash buffer and further loaded onto an ion-exchange column (MonoQ 5/50 GL column, GE Healthcare) to achieve highly pure PBAF complex ...
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bioRxiv - Synthetic Biology 2022Quote: Protein cargo exchange experiments were performed by incubating 100 nM DNA origami with 300nM Alexa-647 labelled protein cargo for 1h before addition to washed s300 resin (according to manufacturer’s instructions, GE Life Sciences) in a spin column and centrifugation for 4 mins at 1000 g to remove free protein ...
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bioRxiv - Microbiology 2022Quote: ... The final protein purification step and determination of oligomeric state was done on a Superose6 Increase 10/300 size exclusion column (GE Healthcare). The column was equilibrated in buffer B and the concentrated protein was loaded onto the column ...
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bioRxiv - Biochemistry 2022Quote: ... The former vector encodes a glutathione S-transferase (GST) fusion protein that can be removed proteolytically with Prescission Protease (GE Healthcare) after affinity purification ...
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bioRxiv - Microbiology 2022Quote: ... Cells that integrated the fluorescent marker were selected on TSA with 15 µg/mL gentamycin and confirmed for their ability to expression the mCherry fluorescent protein using a Typhoon FLA 9000 imaging system (GE Healthcare). The gentamicin resistance cassette was flipped by electrotransformation of the pFLP3a plasmid containing the FLP-recombinase into the newly produced fluorescent cells.
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bioRxiv - Immunology 2022Quote: ... The antibodies were purified from Expi293 cell supernatant by high-performance liquid chromatography (HPLC) coupled with HiTrap Protein A HP columns (Cytiva, formerly GE Healthcare). Monoclonal antibodies were then dialyzed by SnakeSkin dialysis tubing (10K MWCO ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Biochemistry 2022Quote: ... SEC was carried out on a Superose 6 Increase 10/300 GL column by fast protein liquid chromatography (ÄKTA; GE Healthcare); 0.25 mM fractions were collected ...
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bioRxiv - Biophysics 2022Quote: ... Protein concentration of the extracts were determined by SDS-PAGE and densitometry analysis using the LMW-SDS Marker Kit (GE Healthcare) as standard.
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bioRxiv - Cell Biology 2022Quote: ... 500 µg of the purified protein or the complex was loaded on a Superdex 200 10/300 size exclusion column (GE Healthcare) in line with both a DAWN HELIOS II MALS detector (Wyatt Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were separated by SDS–PAGE and transferred to a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences, Marlborough, USA); the membranes were blocked with 1% nonfat milk powder and incubated together with primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Signals corresponding to protein levels were detected using Amersham ECL Prime detection reagent and an Amersham Imager 600 instrument (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... and mAb was purified from spent medium by affinity chromatography on an ÄKTATM pure Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated on 10 % acrylamide gels by SDS-PAGE and protein transferred to a 0.45 μm PVDF membrane (GE Healthcare 15259894) using the BioRad TransBlot Turbo system ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 µL of supernatants were stored as input and the rest were incubated with the lysis buffer-prewashed G-sepharose beads (50 µL of 50% slurry per each reaction; Protein G-Sepharose 4 Fast Flow, GE Healthcare) for 3 h at 4 °C while rotating ...
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bioRxiv - Plant Biology 2021Quote: ... The cells were grown 1 day at 28 °C and two days at 22 °C at 110 rpm The secreted proteins were purified separately by sequential Ni2+ (HisTrap excel, GE Healthcare, equilibrated in 25 mM KPi pH 7.8 and 500 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... normalized protein amounts were resolved under non-reducing conditions by SDS–PAGE and blotted to Hybond-P PVDF membrane (GE Healthcare) for 1 hour at 30 V ...
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bioRxiv - Immunology 2021Quote: ... Affinity-purified proteins were then purified by size exclusion chromatography using a HiLoad 26/600 Superdex 200 pg column (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... was separated from the remaining tag protein and protease by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). The unbound tag-free protein was collected and protein concentration was determined by absorbance (A280 ...
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bioRxiv - Microbiology 2021Quote: ... The final purification of CwlQ-6His protein was conducted via size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using gel filtration buffer (25 mM Tris [pH 8.0] ...
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bioRxiv - Microbiology 2020Quote: ... The concentrated protein was then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare, HiLoad 16/60) equilibrated with buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... amino acids 319-537) (Acro Biosystems, Newark, NJ) protein was measured at 25°C using BIAcore T200 surface plasmon resonance (SPR) (GE Healthcare). STI-1499 or STI-2020 antibody was covalently immobilized on a CM5 sensor chip to approximately 500 and 100 resonance units (RU) ...
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bioRxiv - Microbiology 2020Quote: ... the VHH-72-Fc protein in the cleared cell culture medium was captured on a 5 mL MabSelect SuRe column (GE Healthcare), eluted with a McIlvaine buffer pH 3 ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified to homogeneity by protein A affinity chromatography and characterized by size exclusion chromatography on a Äkta Pure FPLC system (GE Healthcare) with a Superdex S200 10/300 increase column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified by gel filtration chromatography on a HiLoad™ 16/600 Superdex 200 pg column (GE Healthcare) with the following buffer ...
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bioRxiv - Immunology 2020Quote: ... Ab binding (protein expression) was visualized using Amersham ECL Prime Western blotting detection reagent (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). The following Abs were used in this study ...
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bioRxiv - Neuroscience 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Proteins were concentrated and subjected to size-exclusion chromatography on a HiLoad 26/600 Superdex 200 pg (GE Healthcare; Cat# 28989336). Trimer-containing fractions were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... The RBD proteins were further purified with size-exclusion chromatography using a Superdex 200 Increasse 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: The binding affinities of full IgG antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Biophysics 2020Quote: ... The flow-through that contained the protein of interest was then applied to a HiTrap Q 5 mL column (GE Healthcare). The Q column flow-through containing nsp8 or nsp7 was concentrated using a MWCO 3 kDa Amicon Ultra Centrifugal Filter (Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... protein extracts were resolved on SDS-PAGE gels under denaturing conditions and transferred to Hybond ECL membranes (Amersham Biosciences, GE Healthcare). Membranes were blocked for 1 hour at RT and incubated overnight with the respective primary antibody (Supplementary Table S2 ...
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bioRxiv - Microbiology 2021Quote: GST alone and GST-tagged WWOX WW1 and WW2 domains fusion protein were expressed in BL-21 cells and subsequently purified and conjugated to glutathione (GSH) beads (GE HEALTHCARE). HEK293T cells were transfected with eVP40-WT ...