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Citations for GE Life Sciences :
4051 - 4100 of 4723 citations for Recombinant Human FCGR3B Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The gels were exposed to imaging plates and the images of separated tracer-labeled proteins were obtained by reading the plate using a Typhoon 9410 (GE Healthcare) image analyzer ...
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bioRxiv - Biochemistry 2020Quote: IgGs were produced by co-transfection of the heavy chain and light chain pEU vectors (76) in mammalian cells and the IgG1 variants were purified by protein A affinity chromatography (MabSelect Sure columns, GE healthcare) using the AKTA express system and eluted with 0.1 M sodium citrate (pH 3.0) ...
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bioRxiv - Biochemistry 2020Quote: ... Labeling of SNAP fusion proteins and depletion of free dye was confirmed by SDS-PAGE followed by fluorescence imaging on a Typhoon imager (GE Healthcare). Extracts were checked to verify transcription activity (e.g. ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein was diluted with lysis buffer before application to a 5ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), washed with lysis buffer and eluted using buffer supplemented with 2 mM Desthiobiotin.
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bioRxiv - Biochemistry 2020Quote: 100-500 µl samples (lipid-bound and lipid-free ApoE3 and ApoE380-255) were applied to an AKTA protein purification system (GE) equipped with a Superdex 200 10/300 (GE healthcare) column and a 500 µl sample loop and run at 0.5 ml/min at 5°C ...
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bioRxiv - Neuroscience 2021Quote: ... cortices lysates were immunoprecipitated with anti-Bin1 or mouse IgG 16 h at 4 °C and then with 30 μl of protein G–Sepharose beads (GE Healthcare) for 3 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences, Marlborough, MA). After blocking in 5% non-fat dried milk powder (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... Some proteins were further purified by size-exclusion chromatography on a Superose 6 10/300 or Superdex 200 Increase column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... were separated onto a SDS-PAGE and proteins were transferred to Hybond-P PVDF transfer membrane (GE Healthcare, Amersham™ RPN303F) using the manufacturer’s instructions and a Trans-Blot TURBO Transfer System (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... The protein containing fractions were pooled and passed through an ion exchange column (HiTrap® SP FF GE Healthcare Life Sciences) pre-equilibrated in the SP buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The desalted elution fraction was further purified using fast protein liquid chromatography (FPLC) with a HiLoad 16/60 Superdex 200 size exclusion column (GE Healthcare). The buffer used for laforin purification for the small-scale pulldowns described in methods 2.3.2 was (50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: The oligomeric state of proteins as well as protein-protein and protein-tRNA interactions were analyzed by loading 50 μl samples on a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate using a Varian ProStar 335 system (Varian Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were affinity purified on an amylose column and were further purified by ion-exchange chromatography (HiTrap Q HP, GE Healthcare). Protein concentrations were determined by UV spectroscopy ...
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bioRxiv - Cell Biology 2021Quote: ... The mass evaluated using UV as concentration source was 55.7 kDa for the 2 mg/ml sample.The Odinarchaeota samples were analysed by SEC-MALS (100 μl protein complex at 2 mg/ml) were passed over a Superdex 200 10/300 Increase GL column (GE Healthcare), in 20 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted protein was concentrated using Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was concentrated using an Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 mM DTT followed by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was concentrated using an Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT followed by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Fully phosphorylated pNephrin was additionally purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted protein was concentrated using Amicon Ultra 10k concentrators and further purified by size exclusion chromatography to remove any protein aggregates using a Superdex 75 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Fully phosphorylated Cas was additionally purified by size exclusion chromatography to remove any protein aggregates using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... Biotech AB/Sweden) prior to protein separation on a S200 Superdex 10/300GL column on an Äkta Purifier (both GE Healthcare). Subsequently ...
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bioRxiv - Plant Biology 2020Quote: ... Purification tags were then removed by incubation with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant for purified FliWWT/N108Y/K123E/Q124R protein was further cleaned by size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using CsrA gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... His6-SUMO-FliWWT/N108Y/K123E/Q124R protein was further cleaned by size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using CsrA gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... protein used in this study was overexpressed in pET28a and then purified by fast protein liquid chromatography (FPLC; AKTA Explorer 100, GE Healthcare)[19] ...
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bioRxiv - Microbiology 2020Quote: ... Excess DNA was removed from conjugated proteins using size exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) in a 25 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Diluted and filtered samples were then added to a Protein G High Performance MultiTrap 96-well plate (GE Healthcare, Chicago, IL) and shaken at 1,100 RPM for one hour at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare) to remove the excess of copper ...
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bioRxiv - Systems Biology 2020Quote: ... the target-specific antibody was added to the cellular lysates together with 25 μl of Protein A or G sepharose (GE Healthcare) depending on the species of target antibody and the mixture was incubated overnight rotating at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The cleaved protein was finally purified by size-exclusion chromatography (SEC) on a Superose 6 10/300 GL column (GE Healthcare) in 25 mM Tris (pH 8) ...
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bioRxiv - Microbiology 2021Quote: Kinetic measurements of the DH1047 Fab binding to SARS-CoV and RsSHC014 spike proteins were obtained using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Microbiology 2021Quote: ... at a flow rate of 5 ml/min and washed with 600 ml cold column buffer at a flow rate of 10 ml/min on an ÄKTA Fast Protein Liquid Chromatography (FPLC) system (GE Healthcare). Nla28-DBD was eluted using 100 ml cold column buffer containing 10 mM maltose ...
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bioRxiv - Molecular Biology 2021Quote: ... A 12-20% gradient Tris-Glycine SDS-PAGE was used to separate labelled proteins and dried for exposure with a phosphorimager screen and scanned with a Typhoon 9400 or Typhoon FLA 7000 (GE Healthcare) for quantification ...
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bioRxiv - Microbiology 2021Quote: ... The eluted protein was buffered exchanged into endotoxin-free phosphate buffered saline (PBS) using a sterile PD MidiTrap G-25 column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2021Quote: Purified trypsinized post-fusion hMPV B2 F protein was subjected to size exclusion chromatography (S200, 16/300, GE Healthcare Life Sciences) in 50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... The eluted proteins were further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... After cell sonication proteins were affinity purified using a Glutathione sepharose 4b or remains on sepharose for pull down (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
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bioRxiv - Molecular Biology 2020Quote: ... Immobilized Metal Affinity Chromatography (IMAC) was performed for protein purification using an AKTA Start Chromatography system (GE Healthcare Bio-Science, Sweden). The soluble fraction containing TNFα was diluted (1:1 ...
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bioRxiv - Microbiology 2019Quote: ... HSP40 fractions containing purified protein were pooled and further purified via anion exchange using a Mono Q anion exchange chromatography column (GE Healthcare) using an AKTAExplorer 100 FPLC (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... and expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Microbiology 2020Quote: ... Analytical size exclusion chromatography using a Superdex 200 Increase 10/300 column confirmed the absence of aggregates from the protein preparation (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... A second purification step was performed by loading Protein A eluate on a Hiload 16/600 Superdex 200 pg gel filtration column (GE Healthcare), which was equilibrated and run using DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration) together with protein G Sepharose (20 μl of 50% slurry bead volume; GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The protein eluate was concentrated and further purified by size-exclusion chromatography on a Superdex200 10/300 GL column (GE Healthcare) in Buffer C with 0.005% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The His6-tag was removed by thrombin cleavage and the proteins further purified with a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris HCl (pH 7.4) ...