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Citations for GE Life Sciences :
4001 - 4050 of 4264 citations for Recombinant Human PTHLH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: Total IgG antibodies were captured from 2 µL of plasma using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Biophysics 2019Quote: ... TEV-protease digested proteins were passed over Ni-NTA to remove uncleaved proteins and then further purified by size exclusion chromatography using a Superdex200 column (GE Life Sciences) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH5α strain and purification of GST fusion proteins was achieved by incubation with Gluthatione Sepharose 4B matrix (GE Healthcare, Buckinghamshire, UK). Sumo proteins were kept bound to matrix ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Strep Tactin eluted protein was further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Life Sciences) in 25 mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... Visualization of the stained protein on nitrocellulose membranes was carried out by a LAS-4000 Mini luminescence image analyzer (GE Healthcare, Germany) using the ECL Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Free proteins were labeled following the manufacturer’s instructions and purified using Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2020Quote: If necessary the protein breakdown products were separated in the second chromatography step using gel filtration on a Superdex G200 column (GE Healthcare, Freiburg). PBS buffer was used for gel filtration column (137 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein sample was further purified by Size-exclusion chromatography on a HiLoad 16/60 Superdex 75 column (GE Healthcare Life Sciences) with 20 mM sodium phosphate (pH 6.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... Immuno-reactive protein bands were detected by the ECL system and visualized by imaging analyzer (ImageQuant LAS 500, GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... Rabbit sera were shipped to our laboratory where purification of IgG was performed by affinity chromatography using a 5-ml HiTrap protein A HP column following manufacturer’s instructions (GE Healthcare, Piscataway, NJ). Purified IgG protein concentration was determined by Nanodrop ND-1000 spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: ... The protein (5-6 ml) was further purified by FPLC over a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Rosenfeld et al., 2021), expressed using ExpiCHO™ Expression system (Thermoscientific, USA) and purified on HiTrap Protein-A column (GE healthcare, UK). The integrity and purity of the antibodies were analyzed using SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: ... Clarified Expi293F culture supernatants were filtered and Fabs were purified by successive affinity and size-exclusion chromatography (on HiTrap Protein G and Superdex 200 Increase 10/300 GL columns, respectively, from GE Life Sciences) using an AKTA pure system.
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bioRxiv - Biochemistry 2020Quote: The supernatant fraction containing the rhGFAT2 protein (with or without tag) was subjected to purification using a Ni+2NTA affinity column (HisTrap HP 5 mL, GE Healthcare, USA). The column was equilibrated with 10 column volumes (CV ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibodies used for immunoblotting are described in Table S3 HRP-conjugated anti-rabbit secondary antibody was used (Table S3) and protein detection was performed with ECL Select Western Blotting Detection Reagent (GE Healthcare, RPN2235) on an ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... the concentrated linked-L-protein from the Q-Sepharose eluate was subsequently fractionated over a Sephadex S200 26/600 PG (GE Life Sciences) column using STD buffer (100 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: Secreted candidate proteins were purified by immobilized metal ion affinity (IMAC) using Ni Sepharose 6 Fast Flow (GE Healthcare, Chicago, IL, EUA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The resulting proteins were purified using the AKTA start purification system through DEAE Sepharose Fast Flow packed in the XK16 column (GE Healthcare, USA), followed by another purification using Phenyl Sepharose (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... 20 ug of protein extracts for analysis of MT severing enzymes or 2.5 ug of protein extracts for analysis of tubulin proteins were separated under denaturing conditions and transferred to Amersham Protran Premium 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences) prior to blocking in 5% non-fat dried milk in TBS-T for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... containing the cleaved protein, was concentrated (Vivaspin 20, MWCO 5 kDa) and loaded onto a Superdex 75 26/60 column (GE Life Sciences) equilibrated with SEC buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then further purified by size exclusion chromatography using a HiLoad Superdex 75 16/600 gel filtration column (GE Healthcare, USA) equilibrated in 10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... was added and the secreted recombinant Spike or RBD protein in culture supernatant were harvested after 72 hours and affinity purified using HisTrap HP Column (GE Healthcare, USA) as described previously [25] ...
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bioRxiv - Neuroscience 2021Quote: ... the protein Syt C2AB (residues 143 to 421) was purified by gel filtration using a Superdex 75 column (GE Healthcare Life Sciences), as before(73) ...
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bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 14,000 rpm and proteins purified by affinity chromatography using a Ni2+ column (1 mL GE Healthcare HisTrap HP) and imidazole gradient elution in an AKTA Start protein purification system ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of the collected plasma was heat-inactivated (56°C for 40 min) and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Affinity-purified proteins were concentrated and further purified by size-exclusion chromatography (SEC) using a Superdex200 16/60 column (GE Life Sciences) running in 1x TBS (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... The PGT145 affinity purified SOSIP protein was further subjected to size exclusion chromatography (SEC) using a HiLoad Superdex 200 16/60 column (GE Healthcare Inc.). The SEC purified proteins were snap frozen in liquid nitrogen and stored at −80°C until further use ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were filtered through 0.1 um syringe filters and the SiaCP protein was purified using two GraviTrap™ TALON® columns (GE Healthcare). For purification ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of a last step in which the protein was dialyzed against buffer LS and then applied onto a HiTrap SP cation exchange column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... flow-through fractions from the second Ni-NTA affinity purification step containing the protein at high purity were concentrated and then applied onto a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare, USA) equilibrated with a buffer containing 50 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μg of protein per sample were solved in urea lysis buffer and labeled with 250 pmol CyDye DIGE Fluor dyes (GE Healthcare, Germany). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were resolved by SDS-PAGE and proteins were transferred to a nitrocellulose membrane (Cat#10600003, AmershamTM ProtranTM Premium 0.45 μm NC, GE Healthcare Life sciences) which were incubated in blocking buffer (5% nonfat dry milk ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed thrice with PBST and the bands corresponding to protein of interest were detected by chemiluminescence using the Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare; RPN2232) in a Fusion FX7 Spectra Multispectral Imaging system (Witec AG ...
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bioRxiv - Plant Biology 2022Quote: ... or adjusted to Chl/protein content were fractionated by electrophoresis on SDS-polyacrylamide gels (12%) and blotted onto nitrocellulose membranes (Amersham Protran, GE Healthcare, UK). Membranes were stained with Ponceau S and probed with protein-specific antibodies according to (Sambrook ...
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bioRxiv - Microbiology 2023Quote: 0.5 mg of the Ni-NTA protein eluate was analyzed by size exclusion chromatography (SEC) using a Superdex 200 10/300 column (GE Life Sciences) in 25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare, Uppsala, Sweden). Subsequently the membranes were incubated with primary antibodies and HRP-coupled secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then subjected to SDS-PAGE followed by transfer of the proteins onto nitrocellulose membranes (GE Healthcare, Chalfont St Giles, UK). Membranes were blocked in 3% BSA/PBST (1x PBS ...
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bioRxiv - Pathology 2024Quote: ... Proteins were separated by SDS-PAGE (10 % polyacrylamide gel) and transferred to a PVDF membrane (RNP303F, GE Healthcare, Little Chalfont, Buckinghamshire, UK) in a semi-dry blotter (TE70X ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein fraction obtained was further subjected to Hi-Load Superdex 200 pg 16/600 size-exclusion chromatography column (GE Healthcare/Cytiva) equilibrated with buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... The tagged proteins were detected using HRP conjugated sheep anti-mouse or anti-rabbit IgG antibody (1:10000, GE Healthcare, Uppsala, Sweden) and visualized using the Amersham ECL Western blot detection reagent (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then lysed in lysis buffer and equal amounts of their proteins were subjected to Streptavidin beads-based affinity isolation (GE Healthcare, USA). After 30 minutes incubation at 4 degrees ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the desired protein were pooled together and concentrated to 2ml using a 10 kDa cutoff Vivaspin20 concentrator (GE Healthcare Biosciences). Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
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bioRxiv - Biochemistry 2023Quote: ... PD-10 desalting columns (Sephadex G-25 resin), and size exclusion chromatography column (HiLoad® Superdex, 75 pg) used for protein purification were obtained from GE Healthcare. Amorphous ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions with low protein background were pooled and subjected to size-exclusion chromatography on a Superdex 75 pg column (GE Healthcare, USA) in the storage buffer (DesAOMT ...
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bioRxiv - Microbiology 2023Quote: ... The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant; #28-9670-62, GE Healthcare) using binding buffer pH 7 ...
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bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...