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Citations for GE Life Sciences :
3901 - 3950 of 4264 citations for Recombinant Human PTHLH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were then incubated over night at 4 °C with appropriate antibodies (1ug/ml) and protein A Sepharose beads (GE Healthcare). After incubation samples were washed five times with lysis buffer and resuspended in 1X Laemmli sample buffer (Bio-Rad cat ...
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bioRxiv - Biophysics 2023Quote: ... the protein was subjected to ion exchange chromatography and loaded onto a HiLoad 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl with a salt gradient from 0 M to 1.5 M NaCl in buffers containing 25 mM Tris-HCl (pH 7.7) ...
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bioRxiv - Biochemistry 2023Quote: ... After His-tag purification protein was subsequently purified by size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare). The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by gel filtration using fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superdex 200 Increase 10/300 column (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was again washed three times with TBST and the protein of interest was detected using a chemiluminescence reagent (ECL Prime, GE Healthcare). Chemiluminescence was detected using the ImageQuant LAS500 imager (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... insoluble material was removed by centrifugation at 10,000 × g and the cell lysate was pre- cleared by addition of heat inactivated goat serum and protein G sepharose beads (GE Healthcare). Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1 ...
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bioRxiv - Cell Biology 2023Quote: ... Reduced immunoprecipitated proteins were separated on a 10 or 12% SDS-polyacrylamide gel and transferred to a PVDF membrane (GE Healthcare). Details on the blocking of the membranes and detection of the proteins can be found in Table S1 ...
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bioRxiv - Immunology 2022Quote: ... Proteins in gels were either stained with Coomassie blue dye in gel or used for transfer onto nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in PBST (PBS and 0.05% Tween-20 ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were separated by 15% SDS-PAGE and transferred to 0.2 µm Nitrocellulose Blotting Membrane (#10600001, GE Healthcare Life Sciences) by the semi-dry method (Towbin et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010; or Amersham Hybond, GE Healthcare Life Science, 10600023), and immunodetected using primary antibodies directed against HSP60 rabbit polyclonal (D307 ...
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bioRxiv - Neuroscience 2023Quote: ... 3RCF and 4RCF tau proteins were further purified by FPLC using size exclusion Superdex-75 and Superdex-200 columns (GE Healthcare) in 1 x PNE buffer (25 mM PIPES ...
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bioRxiv - Neuroscience 2023Quote: Purified His-tagged DJ-1 and untagged PGK1 proteins were subjected to further purification using gel filtration with a Superdex200 Increase 10/300 GL size-exclusion column (GE Healthcare) on an AKTA FPLC system (Cytiva) ...
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bioRxiv - Plant Biology 2024Quote: ... Co-precipitation of StCWC15a/b with IPI-O1 was examined by protein blotting before (input) and after affinity purification (pull-down) using glutathione agarose beads (GE Healthcare). Interactions were detected using anti-GST and anti-His (Abmart).
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bioRxiv - Biochemistry 2024Quote: ... protein was again passed over Nickel resin to remove His-tagged protein and further purified by size exclusion chromatography (Superdex 75 10/300g, GE healthcare) into 20mM Tris.HCl 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 μg of total protein extracts were fractionated in a 10 % SDS-PAGE followed by a wet transfer to a PVDF membrane (GE Healthcare) [78] ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant loaded to the column and purification was performed by using ÄKTA start protein purification system (GE Healthcare Life Sciences) according to the preferred protocols provided by the purification column manual ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superose 6 Increase size exclusion chromatography column (10/300 prep grade, GE Healthcare). Elution was carried out with 150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant antibodies were produced in a transient HEK expression system and purified via protein A affinity purification on an automated AKTA system (AKTA explorer FPLC, GE Healthcare). For in vivo studies rabbit V-regions were fused with murine constant of mouse IgG2a heavy chain and kappa chain to minimize immunogenicity ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing the protein of interest were concentrated and further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The 6×His-tagged SUMO fusion proteins were purified using a HisTrap™ HP Ni2+ affinity column (GE Healthcare Life Sciences). The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... 200μl of the protein mixtures were analyzed by size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare) using a GE ÄKTA FPLC™ (Fast Protein Liquid Chromatograph ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was further purified by size exclusion chromatography (SEC) with a HiLoad 26/60 Superdex 75 pg column (GE Healthcare) in SEC buffer (20 mM Sodium-phosphate buffer pH 6.5 ...
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bioRxiv - Biophysics 2024Quote: ... to remove metal ions and polysulfane sulfur before proteins were subjected to anaerobic size exclusion chromatography (SEC) (HiLoad 16/600 Superdex; GE Healthcare) on an Äkta Purifier system (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (AmershamTM Protran® 0.45 µm, GE Healthcare Chicago, IL, USA). Membranes were blocked for 1 hour with 5% BSA in TTBS (15 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The TEV-cleaved protein was recovered by second immobilised metal affinity chromatography (IMAC) and further purified by SEC (S200 16/60 column, GE Healthcare) in SEC buffer under isocratic flow ...
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bioRxiv - Immunology 2024Quote: Tested antibody (5 μg/mL for NTD and 1 μg/mL for spike) was captured on protein A sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified SARS-CoV-2 mutant spike or NTDs ...
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bioRxiv - Bioengineering 2024Quote: ... The obtained filtrate was then applied to a Ni-NTA column using a fast protein liquid chromatography (FPLC) system from GE Healthcare. The column was equilibrated with potassium phosphate buffer A (100 mM ...
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bioRxiv - Biophysics 2024Quote: ... and the (His)6-tagged protein was purified using affinity chromatography with Nickel nitrilotriacetic acid (Ni-NTA) according to the manufacturer’s recommendations (GE Healthcare, UK). SDS-PAGE analysis validated the purification of rESAT-6 ...
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bioRxiv - Developmental Biology 2024Quote: ... The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3) and purified using glutathione Sepharose 4 B (GE Healthcare). An antibody against Ccrk was obtained by immunizing guinea pigs with purified GST-fused Ccrk.
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bioRxiv - Synthetic Biology 2024Quote: ... and those found to contain the purified protein were pooled and concentrated using a 10 kDa molecular weight cut-off Vivaspin spin column centrifugal concentrator (GE Healthcare) at 3894 x g and 4°C ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: ... Abcam)] overnight in rotating conditions followed by incubation with 50 µl of 50% Protein A Sepharose-beads (GE Healthcare,17-0780-01) for 2 hrs at 4°C in rotating condition ...
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bioRxiv - Biochemistry 2024Quote: ... for 18 h at 18°C and N-terminal His6-tag fusion proteins purified by step-wise affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM tris-HCl (pH 7.4) ...
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bioRxiv - Biochemistry 2024Quote: ... the excess unreacted dye was removed from each protein by passing it through a 600 μl Sephadex G-25 Fine resin (GE Healthcare), pre-equilibrated with a buffer containing 20 mM Hepes-K pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... The denatured proteins were resolved on 8–12 polyacrylamide-SDS gels and transferred onto PVDF membrane (GE Healthcare, Chicago, IL, USA). A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color ...
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bioRxiv - Microbiology 2024Quote: ... The protein was further purified using size exclusion chromatography with a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare) into a final buffer of 20 mM HEPES pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was separated on an 8-16% gradient Tris-glycine gels and transferred to PVDF blotting membrane (GE Healthcare Life Sciences). The membrane was immunoblotted with 1:200 anti-FGF13 antibody or 1:200 anti-pan FHF-A (Neuromab clone N235/22 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein detection and quantification were performed using the SYBR-green fluorescence detection mode of the LAS-4000 image analyzer (GE Healthcare) and Image Quant TL software (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were detected with host-specific secondary antibodies and developed with a chemiluminescent substrate (GE Healthcare Amersham, Piscataway, NJ, USA). After stripping ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Biophysics 2024Quote: ... monomeric and tetrameric protein were separated and collected by SEC using a Superdex 75 Increase 10/300 GL column (GE Healthcare) and then used directly in fluorescence experiments (SI Appendix ...
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bioRxiv - Biophysics 2024Quote: ... TEV-cleaved protein was then diluted 1:500 in KBT and then loaded onto a HiTrap Q HP column (GE Healthcare) for ion exchange chromatography (IEC ...
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bioRxiv - Plant Biology 2024Quote: ... His6-tagged proteins (His6-HsfA2/S-HsfA2/S-HsfA4c/S-HsfB1/S-HsfB2a/DBD/tDBD) and His6 mock proteins were purified using glutathione Sepharose 4B beads (GE Healthcare) and nickel-nitrotriacetic acid (Ni-NTA ...
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bioRxiv - Microbiology 2024Quote: ... Full-length untagged cGAS protein was then ultimately purified using size-exclusion chromatography via a 16/600 Superdex S75 column (GE Healthcare). Purified cGAS was concentrated to ∼10 mg/ml in storage buffer (20 mM HEPES-KOH pH 7.5 ...