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Citations for GE Life Sciences :
351 - 400 of 2258 citations for Collagen 17 alpha 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The immuno-complexes were then incubated with 30 μl protein G sepharose beads (GE healthcare, 17-0618-01), which were pre-equilibrated with Lysis Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then incubated with 30 µl 50% protein A-Sepharose slurry (GE Healthcare, 17-0780-01) for 1 hr at 4 °C ...
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bioRxiv - Biophysics 2024Quote: The yeast tRNAPhe from Sigma was labeled on its dihydrouridine (D) residues at the 16/17 positions using Cy3-hydrazide from GE Healthcare. This process involved first reducing the D residues with sodium borohydride (NaBH4 ...
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bioRxiv - Bioengineering 2019Quote: ... The peptides were passed through Illustra Microspin G25 columns (GE Healthcare) to remove TCO-PEG3-maleimide.
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bioRxiv - Molecular Biology 2023Quote: Peptide pulldowns were performed using Streptavidin High Performance beads (GE Healthcare) and either RIPA or EBC buffer ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Microbiology 2020Quote: ... the conditioned media was collected by centrifugation followed by purification using HisTrapTM HP (Cat: 17-5248-01, GE Healthcare). The purified protein was buffer exchanged into PBS using a Vivacon 500 concentrator (Cat ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and bound to Glutathione sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-02). Beads were washed several times with lysis buffer and protein was eluted from beads with 10 mM reduced glutathione in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was incubated with Glutathione Sepharose beads (20 μL for one group, 17-0756-01, GE healthcare, Sweden) at 4 °C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... Matrical Bioscience) were coated with a filtered solution of Concanavalin A (0.5 mg/ml, 17-0450-01, GE Healthcare) for 30min ...
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bioRxiv - Molecular Biology 2019Quote: Clarified extracts containing hexahistidine fusion proteins were passed through a 5 mL Ni-Sepharose (GE Healthcare #17-5268-01) packed column at 0.5 mL/min using an ÄKTAprime Plus chromatography system ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts containing GST fusion proteins were passed through a 5 mL GSTrap column (GE Healthcare #17-5131-02) at 0.1 mL/min using an ÄKTAprime Plus chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The medium from 1 L of culture was filtered through a 0.22-μm membrane and incubated with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) in the presence of 20 mM of imidazole for 2-3 h at 4 °C with mild agitation ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was loaded on a gravity chromatography column preloaded with glutathione-Sepharose 4B (17-0756-05, GE Healthcare) and incubated for 2 h at 4 °C with gentle rotation in batch mode ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Samples were further purified by ion exchange on a HiTrap Q column (GE Healthcare Life Sciences #17-1153-01) and eluted in 250 μl aliquots of 20/250 TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The soluble fraction of the cell lysate was purified on HiTrap IMAC HP columns (GE Healthcare # 17-0920-05) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... GST-hMeCP2 or GST-OGT fusion proteins were immobilized on glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) and washed three times with GST binding buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant GST-tagged protein in the supernatant was purified using glutathione-Sepharose 4B beads (GE Healthcare, 17-0756-01), washed in 10 mM PBS (pH 7.4 ...
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bioRxiv - Immunology 2019Quote: PBMCs were isolated from whole human peripheral blood by Ficoll-Paque PLUS (17-1440-03, GE Healthcare, Pittsburgh, PA) density gradient centrifugation and cryopreserved prior to use ...
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bioRxiv - Immunology 2019Quote: ... the cells were resuspended in approximately 2 mL of 100% Percoll solution (Cat. no. 17-0891-01, GE Healthcare), and layered carefully on top of 3 mL of 80% and 40% Percoll solution ...
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bioRxiv - Cell Biology 2021Quote: ... 300 mM KCl was performed using a PD-10 desalting column (GE Healthcare, Chicago, IL, Catalog #17-0851-01). Protein was then concentrated again and total protein content was determined by the Bradford method using the Protein Assay Dye Reagent (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Which Peripheral blood after dilution were subjected to lymphocyte enrichment using Ficoll-Paque Plus (17-1440-02, GE Healthcare). The peripheral blood leukocytes were further purified by fluorescent activated cell sorting with 7-AAD (420404 ...
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bioRxiv - Immunology 2020Quote: ... culture media was collected and incubated overnight at 4°C with nickel-Sepharose Excel (GE Healthcare 17-3712-01). His-tagged proteins were eluted from the nickel-Sepharose with 1 M imidazole in TrisCl pH 8.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies and associated DNA were then isolated using Gammabind G Sepharose beads (GE Healthcare Bio, catalog # 17-0885-01). Crosslinks were reversed by incubating samples and input at 65°C for at least 6 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... and IgGs were then purified from the culture supernatants by Protein A-column chromatography (GE Healthcare, 17-1279-01). We obtained 6 clones as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Fibronectin was isolated from human plasma using Gelatin-Sepharose 4B (Cat No. 17-0956-01, GE Healthcare, Sydney, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was centrifuged and leukocytes were further separated using 40% Percoll density gradient (GE Healthcare Cat# 17-0891-01) and centrifugation (1400 x g ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked in 2% ECL Prime™ Blocking Agent (GE Healthcare, RPN418) in TBST (Tris-Buffered Saline (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... The sample was fractionated on Superdex Peptide PC3.2/30 column (GE Healthcare) at a flow rate of 50 µl/min of 30% ACN ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by gel-filtration chromatography on Superdex Peptide 10/300 (GE Healthcare) in Buffer A.
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bioRxiv - Cell Biology 2019Quote: MTED peptide was coupled to cyanogen bromide-activated Sepharose beads (GE Healthcare). 30μM porcine brain tubulin was incubated with either peptide-coated or uncoated Sepharose beads in BRB80 ...
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bioRxiv - Biophysics 2022Quote: ... the sample was passed through Superdex peptide 10/300GL column (GE healthcare); connected to Biologic Duoflow model Bio-Rad fast protein liquid chromatography (FPLC ...
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bioRxiv - Cell Biology 2024Quote: ... peptides were loaded to an Immobiline™ DryStrip Gel (GE Healthcare, #11534985) isoelectric focusing (IEF ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... Alpha-synuclein was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction column (GE Healthcare) (76) ...
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bioRxiv - Biophysics 2022Quote: ... Eluted Sox2 was refolded by changing to a zero-urea buffer using a desalting column (GE healthcare #17-1408-01). Further purification was performed by gel filtration on a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2019Quote: Cells for single cell RNA-seq were prepared by density centrifugation using Ficoll-Paque media (GE Healthcare, 17-5446-02) followed by magnetic-activated cell sorting (MACS ...
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bioRxiv - Biochemistry 2020Quote: ... Medium was collected 48-60 h post infection and incubated with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) pre-equilibrated with Buffer A (150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Cancer Biology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll®-Paque PLUS, GE Healthcare, 17-1440-03) from healthy donors ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was applied to a 5-ml His-Trap HF column charged with NiCl2 (GE Healthcare, 17-5248-02). GFP-NONO_WT or GFP-NONO_ΔRRM1 was eluted at room temperature with an imidazole gradient (25-500 mM ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were purified by a density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare Life Sciences, Cat# 17-1440-03) and stored in liquid nitrogen until further use.
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was incubated with either Glutathione Sepharose® 4B (for GST-tagged proteins, GE Healthcare, #17-0756-01) or Protino Ni-TED resin (for SUMO-tagged proteins ...
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bioRxiv - Biochemistry 2021Quote: ... The medium from 1 L of culture was filtered using a 0.22-μm membrane and the filtrate was adjusted to contain 30 mM imidazole before incubating with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) beads for 2 h at 4 °C with mild agitation ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were isolated from EDTA blood using density gradient separation in Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were incubated with 5 ml bed volume Glutathione Sepharose 4B resin (GE Healthcare, cat. No. 17-0756-05) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... After loading the cell lysate onto a Protein L beads (Capto™ L, GE Healthcare, Cat. no. 17-5478-02) column ...
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bioRxiv - Cancer Biology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation using Ficoll-Paque Premium (#17-5442-02; GE Healthcare). PBMCs were washed 3x with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Superose 6 (10/300) column (#17-5172-01) and Superdex 200 increase 3.2/300 column (#28-9909-46) from GE Healthcare; Trans-blot turbo transfer pack (#1704158 ...