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Citations for GE Life Sciences :
451 - 500 of 2258 citations for Collagen 17 alpha 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Crosslinked products were enriched by size-exclusion chromatography (Superdex Peptide, GE Healthcare Life Sciences) as described previously34 and fractions eluting between 0.9 and 1.4 mL were dried ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were purified via GST affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The GST tag was proteolytically cleaved by TEV-protease digestion ...
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bioRxiv - Biochemistry 2023Quote: ... Unreacted fluorescent peptide was removed using the Superdex 75 size exclusion column (GE Healthcare) in 50 mM TRIS pH 8 ...
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bioRxiv - Physiology 2019Quote: ... Equal loading of protein content was verified using alpha-tubulin as a housekeeping protein and bands visualised using ECL detection system (GE Healthcare, UK). Bands were measured using Image J densitometry ...
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bioRxiv - Neuroscience 2021Quote: ... Free calcein or SST was removed by size-exclusion chromatography with Sephacryl® S-1000 column (Cat. # 17-0476-01, GE Healthcare). Second ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in 24 mL of RT 70% PercollTM (1.088 g/mL at 22 °C; GE Healthcare Life Sciences 17-0891-01). Aliquots of 8 mL cell/PercollTM suspension were overlayed with 4 mL HBSS and centrifuged at 400 xg for 30 minutes RT ...
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bioRxiv - Biochemistry 2020Quote: ... The percent monomer was determined by dividing the estimated area under the monomer peak (elution volume of ∼15.4 to 17 mL) with the total area (monomer + dimer) using UNICORN software (default software of AKTA FPLC, GE Healthcare Life Science), followed by multiplication by 100.
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bioRxiv - Cell Biology 2021Quote: ... 500μl of dialyzed GST-Bsd or GST was bound to 50μl of PBS washed glutathione sepharose beads (GE Healthcare, 17-0756-01) and incubated at 4°C for 30min ...
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bioRxiv - Cell Biology 2021Quote: ... A volume of 2.5 ml of soluble fraction was incubated with 100 μl of Glutathione Sepharose beads (GE Healthcare, 17-0756-01) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The peak fractions were pooled and loaded onto a Superdex 200 HiLoad 16/60 preparative-grade column (GE Healthcare, 17-1069-01) in 5 mL injections and developed at room temperature with the size exclusion buffer (20 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
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bioRxiv - Plant Biology 2021Quote: ... and fractionated by gel permeation chromatography (GPC) using a Superdex 200 10/300 GL column (GE Healthcare, catalog no. 17-5175-01) connected to an AKTA FPLC system (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... We performed immunoprecipitations with precoated anti-SRSF9 or rabbit normal IgG-protein A Sepharose beads (GE Healthcare, catalog number: 17-1279-02) for 2 □ hours at 4□°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein from each sample were added to 50 µl of precleaned CaM binding beads (GE Healthcare, 17-0529-01) in 300 to 500 µl of 1% TX100-TNM lysis buffer and incubated with rotation at 4°C overnight (16-18 hours) ...
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bioRxiv - Biochemistry 2019Quote: ... 0.0027M KCl pH 8) and they were bound to a GST affinity column (GSTrap™ Fast Flow, GE Healthcare: 17-5131-02). The column was washed (0.02M NaPO4 ...
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bioRxiv - Developmental Biology 2019Quote: ... The mixture was layered on 40 ml of 5% Percoll (cat no. 17-0891-02, GE Healthcare Bio-sciences AB, Uppsala, Sweden) in HBSS (cat no ...
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bioRxiv - Neuroscience 2021Quote: ... human peripheral blood mononuclear cells were isolated from buffy coats obtained from healthy volunteers (Niguarda Hospital) after Ficoll®Paque Plus (Cat. 17-1440-02, GE Healthcare) density gradient centrifugation ...
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bioRxiv - Microbiology 2023Quote: Blood was drawn from five healthy donors and PBMCs were isolated using Ficoll-Paque Plus (17-1440-02, GE Healthcare, Danderyd, Sweden) gradient centrifugation ...
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bioRxiv - Bioengineering 2023Quote: ... and the ends of the image were cropped flat to a length of 17 mm (MicroView ABA 2.1.1., GE Healthcare, London, Ontario, Canada). Past work has suggested that the use of a global segmentation threshold for a cohort with a broad range of bone volume fraction or varying bone mineral density can result in an inaccurate topology (Bouxsein et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated by density gradient centrifugation using the Ficoll® Paque Plus solution (17-1440-02, GE Healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... U87vIII CHCHD2 WT and KO cells were seeded onto collagen-coated dextran beads (∼200 µm diameter, GE Life Sciences, Pittsburgh, PA) at a density of 2 × 106 cells per 5 × 103 beads in 5 mL DMEM ...
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bioRxiv - Biophysics 2021Quote: ... and the excess small molecular was removed by Superdex peptide size-exclusion column (GE Healthcare) equilibrated in refolding buffer ...
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bioRxiv - Immunology 2019Quote: ... Bound biotinylated peptide was detected by addition of streptavidin-alkaline phosphatase conjugate (GE Healthcare, France) and 4-methylumbelliferyl phosphate substrate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Cross-linked peptides were enriched in a Superdex 30 Increase 3.2/300 column (GE Healthcare) equilibrated with 30% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked peptides were enriched by size exclusion chromatography using an ÄKTAmicro chromatography system (GE Healthcare) equipped with a Superdex™ Peptide 3.2/30 column (column volume = 2.4 ml) ...
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bioRxiv - Microbiology 2021Quote: ... the peptide was purified with size-exclusion chromatography using a Superdex 75 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Na HEPES ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... and purified the MTBD constructs from the lysates using a HisTrap High Performance nickel Sepharose column (17-5247-01, Cytiva (formerly GE Healthcare Life Sciences), Marlborough ...
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bioRxiv - Cancer Biology 2020Quote: ... to separate blood constituents and isolation of buffy coat density gradient centrifugation (400 × g at 18°C for 30 min) of RPMI-1640 diluted blood over a Ficoll-Paque(tm) Plus reagent (Cat. 17-1440-02, GE Healthcare, Piscataway, NJ) was performed ...
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were affinity-purified from filtered cultured supernatant on a High-Trap protein G column (GE Healthcare, Chicago, USA, Cat.Nr 17-0404-01).
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bioRxiv - Immunology 2022Quote: ... and the resultant lysate was incubated overnight at 4 °C with 1.5 ml of glutathione-Sepharose 4B bead slurry (GE Healthcare, 17-0756-01). Beads were washed three times in wash buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
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bioRxiv - Microbiology 2022Quote: ... bacterial extracts were centrifugated for 20 min at 18,000 x g at 4°C and the supernatant was incubated with 1.5 ml of a GSH-sepharose 4B resin (GE Healthcare 17-0756-01) for 1.5 h at 4°C on a rotating wheel ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were further isolated using a Percoll® gradient: samples were resuspended in 45% Percoll® (GE Healthcare, Cat. No. 17-0891-01), prepared in DMEM + 1.5M NaCl) ...
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bioRxiv - Biochemistry 2023Quote: ... The TEV protease cleaved PIK3CB/ybbr-PIK3R1 complex was separated on a Superdex 200 size exclusion column (GE Healthcare, Cat# 17-5174-01) equilibrated with 20 mM Tris [pH 8.0] ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of supernatant from each sample were mixed with 30 μl of pre-washed streptavidin-sepharose beads (GE Healthcare, 17-5113-01) and incubated for 3 hours at 4°C on a nutator ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were red cell and granulocyte depleted by density gradient separation using Ficoll-Paque PLUS (GE Healthcare Life Sciences, cat. no. 17-5442-03) and CD34 enrichment was carried out on freshly isolated mononuclear cells (MNC ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified extract was either directly loaded onto Superose 6 column following pre-clearing with 150 μl of Sepharose CL-2B (GE Healthcare, 17-0140-01) or subjected to Halo purification by incubating with 100 μl of HaloLink™ Resin (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Immunology 2021Quote: ... Whole blood was collected 25 to 39 days following a positive RT-PCR test and subjected to density gradient centrifugation using the Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll density gradient using Ficoll Plaque Premium (Sigma GE Healthcare, #17-544-02) or BD Vacutainer CPT Tubes (BD ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in 10 ml FACS wash and filtered through a nylon filter before being overlayed onto 3 ml Ficoll (GE Healthcare, 17-1440-03). Cell/Ficoll mixture was centrifuged at 931g for 1 hour at room temperature with brakes off ...
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bioRxiv - Pathology 2022Quote: Epoxy-activated sepharose 4B conjugated with tegaserod maleate was prepared according to the manufacture’s instruction (#17-0480-01, GE Healthcare Life Sciences, Uppsala, Sweden). 200 μl tegaserod maleate-Sepharose 4B beads or vehicle were rotated with cell proteins (500 μg ...
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bioRxiv - Bioengineering 2019Quote: The TetR-family protein protein CAETHG_0459 was eluted in the same wash buffer containing a stepwise imidazole gradient (50-500 mM) following a buffer exchange performed using a HiTrap Desalting column (GE Healthcare #17-1408-01). Finally ...
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bioRxiv - Immunology 2020Quote: ... Whole blood was collected 31 and 32 days following a positive RT-PCR test and subjected to density gradient centrifugation using the Ficoll Paque Plus reagent (GE Healthcare, #17-1440-02). After separation ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation procedures were performed by mixing ~1.6 mg of total proteins and 30 μl of the IgG Sepharose 6 Fast Flow beads (GE Healthcare, Cat# 17-0969-01) at 4°C for 2h and precipitating the NTAP tagged Orc4 ...
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bioRxiv - Plant Biology 2020Quote: ... Suspension was gently load onto two prepared falcon tubes containing equal volumes of 2X isolation buffer and Percoll™ (GE Healthcare-17-0891-02). Samples were centrifuged in a swing bucket centrifuge at 1200Xg for 10 minutes with brakes off ...
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bioRxiv - Genomics 2021Quote: ... #S0282).[1] Reverse transcription was performed in 1.5 mL microfuge tubes under end-over-end rotation using a modified Reverse Transcription mix (1x Maxima H-RT buffer, 4% Ficoll PM-400 (GE Healthcare, #17-0300-05), 3 mM MgCl2 (Sigma ...