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Citations for GE Life Sciences :
3851 - 3900 of 4276 citations for Recombinant Human Pro NGF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Crescendo and Forte Reagents and Amersham Imager 600 (GE Healthcare). Quantitative densitometric analyses were performed using ImageJ software.
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bioRxiv - Cell Biology 2024Quote: ... We pooled the α-syn-containing fractions and further purified the protein by size-exclusion chromatography on a HiPrep Sephacryl S-200 HR 16/60 gel filtration column (GE Healthcare) in 50 mM NH4Ac pH 7.4 ...
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bioRxiv - Immunology 2024Quote: IgGs were purified from ∼2 mL of plasma with an equal volume of Protein A Sepharose resin (GE Healthcare Life Sciences) and incubated with the resin for three days at 4°C ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Microbiology 2024Quote: ... Concentrated proteins were further purified by Size-exclusion chromatography (SEC) either using a HiLoad 16/60 Superdex 200 pg column (GE Healthcare) or a HiLoad 16/60 Superdex 75 pg column (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... These eluted proteins were subsequently passed through a superdex s75 16/60 column attached to an AKTA Pure system (GE healthcare) in 50 mM Tris pH 8.0 and 300 mM NaCl.
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bioRxiv - Microbiology 2024Quote: ... The lysate underwent a subsequent centrifugation at 20,000 □g for 45 □min and the cleared soluble protein lysate was loaded on a prepacked Ni-NTA column (GE Healthcare). Proteins binding to the Ni-NTA coloumns were washed with 20 column volumes of binding buffer supplemented with 20 □mM imidazole to eliminate impurities ...
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bioRxiv - Microbiology 2024Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2024Quote: ... protein was further purified by size exclusion chromatography on a HiPrepTM 26/60 SephacrylTM S200 HR gel filtration column (GE Healthcare). Protein was eluted isocratically at a flow of 1 ml/min ...
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bioRxiv - Bioengineering 2024Quote: ... SpyTag003-linked non-antigen binding proteins) were injected onto a pre-equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare), or a HiLoad 16/600 Superdex 75 pg column (SUMO-SpyTag003 and affibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten micrograms of protein was separated on SDS-PAGE gels (10% acrylamide) and wet-transferred to a PVDF membrane (GE Healthcare). Membrane blocking was performed with 5% skimmed milk in Tris-buffered saline (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Crescendo and Forte Reagents and Amersham Imager 600 (GE Healthcare). Quantitative densitometric analyses were performed using ImageJ software.
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bioRxiv - Microbiology 2023Quote: ... HA-UL47-containing protein complexes were immunoprecipitated by incubating extracts with HA-specific antibodies and protein G-coupled sepharose beads (4 Fast Flow, GE Healthcare). The sepharose-bound proteins were analyzed by standard immunoblotting for the presence of HA-UL47 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by size exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein purification was conducted using a combination of affinity and size-exclusion chromatography (SEC) performed using Äkta Purification systems (GE Healthcare). Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: Further protein purification was achieved by size exclusion chromatography (SEC) through a Superdex™ 200 10/300 GL column (GE Healthcare) using SEC buffer containing 0.5 M NaCl and 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Tev protease and other contaminants were removed with Nickel NTA resin and the unbound protein purified further using a HiLoad 16/60 superdex 75 size exclusion column (GE Healthcare). His-GST-DIDATRX ...
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bioRxiv - Biochemistry 2022Quote: ... To insure the monodispersity of samples all proteins were subjected to SEC directly before collecting of scattering data on a Superdex 200 10/300 GL (GE Healthcare) equilibrated with 20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... and the resulting compounds were purified from cell culture supernatants using an kta Express chromatography system using affinity purification using Mabselect Sure Protein A resin followed by a gel filtration column based on the Superdex200 resin (GE Healthcare). The MabSelect Sure Protein A column was washed with 0.1 M Hepes ...
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bioRxiv - Biophysics 2022Quote: ... Size exclusion chromatography was performed on the concentrated protein using a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in gel filtration buffer (10 mM Na2HPO4.7H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... The SUMO-tagged or cleaved protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 25 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The SUMO-tagged or cleaved protein were concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 20 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... 3 mg of proteins was used for immunoprecipitation with 50 ml of immunoglobulin G-Sepharose resin (IgG Sepharose 6 Fast Flow, GE Healthcare) or Anti-HA affinity matrix beads (Roche ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was clarified using 0.45 µm filters before loading onto a nickel-Sepharose Fast Flow column (5 mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The column was washed with 40 mL of extraction buffer ...
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bioRxiv - Microbiology 2023Quote: ... the negatively charged N-terminal His- and BAP-tag fused MBP protein was separated from HphA by ion-exchange chromatography with HiTrap Q HP 5ml column (GE Healthcare). The flow-through ...
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bioRxiv - Plant Biology 2023Quote: ... The protein sample was then purified using size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), previously equilibrated with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... and the light SILAC labeled sample (transfected with individual constructs) followed by enrichment of biotinylated proteins by Streptavidin Sepharose High Performance affinity resin (GE Healthcare). For all steps ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were cleared by centrifugation and the soluble protein fraction was affinity-purified on a HisTrap Excel column (GE Healthcare). After elution in a 0–300 mM imidazole gradient ...
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bioRxiv - Microbiology 2023Quote: ... and the protein was eluted with an imidazole gradient (300 mM-500 mM) using the AKTA prime chromatography system (GE Healthcare). Fractions containing pure ParR protein (26.5 kDa ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences) pre-equilibrated with buffer F (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The membrane was then washed several times in 0.05% TBS-T and proteins were detected using enhanced chemiluminescence (ECL) reagent (GE Healthcare; RPN2106). Antibodies used include ICAM-1 (Cell Signaling Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Equal Volume of total soluble proteins were separated on a 15% (w/v) SDS polyacrylamide gel and transferred onto a Hybond-P membrane (RPN303F, GE Healthcare). Nonspecific binding was blocked with 3% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.4 400 mM Imidazole) The labeled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex-75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove other contaminants and soluble aggregates ...
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bioRxiv - Biophysics 2023Quote: ... the protein was diluted 10 times with PBS buffer and immobilized on a Nickel Sepharose 6 Fast Flow resin (GE Healthcare). The resin was washed extensively with milliQ water followed by PBS buffer pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated and was further purified by a Superdex increase 200 16/600 size exclusion chromatography column (GE Healthcare) that was pre-equilibrated with SEC buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The following morning unbound G protein and Nb35 were separated from the complex by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with the buffer containing 20 mM HEPES/KOH pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... lysed with a high-pressure homogenizer and Fab was purified with a 5-ml HiTrap Protein G HP column (GE Healthcare). Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0 ...
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bioRxiv - Biophysics 2023Quote: ... Proteins separated based on molecular weight onto the gel were transferred onto a 0.2 μm western blot membrane (GE life sciences) using a thermo scientific transfer cassette for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 35 μg protein from each sample was resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). The following antibodies were used at the indicated dilutions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using SDS-PAGE in 10 % Bis-Tris acrylamide/ bisacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). The membranes were blocked for 30 minutes with PBS 5 % milk and incubated with the first antibody for 1 hour at room temperature or at 4°C over-night with gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... The protein elutes in the flow through and is subsequently subjected to size exclusion chromatography using a Superdex 200 (GE Healthcare) in a buffer containing 50 mM HEPES (pH 8.2) ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein of interest was then purified by size exclusion chromatography using a Superdex 75 prep grade column (GE Healthcare). Where necessary ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP tag was cleaved off by TEV protease and the protein was further purified by a passage through a Ni2+-Chelating Sepharose (GE Healthcare). IHO1 was then purified by a gel filtration on Superdex 200 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biophysics 2023Quote: Ion exchange chromatography (IEC) was performed using an ÄKTA Purifier protein purification system controlled by the software Unicorn 3.10 (GE Healthcare, Sweden). CIMmultus QA 1 mL column (BIA Separations ...