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Citations for GE Life Sciences :
3801 - 3850 of 4276 citations for Recombinant Human Pro NGF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... His6-tagged SECTO-5GS-1TD0 spike protein was extracted from the supernatants using an immobilized Ni SepharoseTM Excel column (GE Healthcare) and eluted with 500 mM Imidazole prior to SEC ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC) using five milliliter His-Trap HP columns (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Microbiology 2022Quote: ... Both the 6His- and Strep-tagged proteins were then further purified by SEC on an ÄKTA Purifier using a Superdex 200 16/600 GL column (GE Healthcare), and simultaneously buffer-exchanged into 50 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mg of total protein was pre-cleared for 3h at 4°C by incubation with glutathione-coupled sepharose beads (GE Healthcare) previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed on HisTrap FF crude 5 ml Ni-based column using ÄKTA Xpress System (Cytiva, former GE Healthcare). For that a wash buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated using centrifugal concentrators (Amicon, 100 kDa) and polished by size exclusion chromatography (SEC, Superose 6 Increase, GE Healthcare) in buffer containing 25 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... ∼1 mg of protein was desalted into 100 mM sodium bicarbonate (NaHCO3) at pH 8.3 using a PD10 desalting column (GE Healthcare 17085101) according to manufacturer’s suggestions ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved TPR-CHAT protein was eluted from GST beads and further fractionated by a Resource Q (AKTA Pure, GE Healthcare) in buffer ...
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bioRxiv - Bioengineering 2022Quote: ... all proteins were then further purified by size exclusion chromatography using a Superdex 75 10/300 GL increase column (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing the proteins were pooled and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and dArc1 and dArc2 proteins were further purified by size exclusion chromatography using Superdex S200 16/600 (GE Healthcare Life Sciences).
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bioRxiv - Cell Biology 2022Quote: ... sjRop1 LPPs and the purified REEP1 proteins from the membrane fractions were further purified by size exclusion chromatography (SEC) on Superose 6 or Superdex 200 (GE Healthcare) columns ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was harvested and the protein was purified by FLAG-affinity purification followed by MonoQ anion exchange chromatography (GE Healthcare). Finally ...
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bioRxiv - Plant Biology 2022Quote: The purified protein from untagged OscobB was further analysed by a size exclusion chromatography (SEC) passing 0.5mg protein through the SUPERDEX 200 (10/300 GL) column (GE Healthcare, USA) attached to AKTA pure 25 at the rate of 1ml/min ...
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bioRxiv - Microbiology 2022Quote: ... Eluates were concentrated by centrifugal filtration and proteins were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare) with running buffer PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The concentrated protein is dialysed against buffer A which was further subjected to purification using the gel-filtration sepharose column (GE healthcare) and collected using an automated AKTA collector ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing purified MglA-His6 or His6-MglB proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer A without imidazole for use in GTPase assays or buffer C (20mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ...
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bioRxiv - Microbiology 2022Quote: ... Proteins (40 µg) were run on 10% SDS–PAGE gel and transferred to an Amersham Hybond-ECL nitrocellulose membrane (GE healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The 6xHis-tagged protein was purified from the insoluble fraction of a separately induced culture using Ni-NTA beads (GE Healthcare) with 250 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... A total of 100 μl containing 10 mg/ml of disassembled protein were loaded onto a Superdex 75 Increase 10/300 GL (GE Healthcare) using 300 mM NaCl for elution ...
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bioRxiv - Plant Biology 2022Quote: ... Purification tags were then removed by incubating with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... Separation of cleavage tags and aggregated proteins was performed using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20□mM citric acid pH 5.0 ...
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bioRxiv - Plant Biology 2022Quote: ... equal amounts of proteins were separated on a 10% or 12% SDS-polyacrylamide (PA) gel and transferred to nitrocellulose membranes (GE Healthcare). The signal was probed with specific antibodies using Clarity Western ECL Blotting Substrate (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare) eluting in PBS with 1 mM DTT ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was filtered on 0.45 µm units before loading onto a nickel-Sepharose Fast Flow column (1mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The PLP protein was eluted at 160 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: 4 mg of native protein standard was loaded to calibrate the gel filtration column (Superdex 200 increase 10/300 GL column, GE Healthcare). Following this ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Physiology 2022Quote: ... Equal amounts (30 μg) of total protein were separated by 10 % SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). After blocking with Tris-Buffered Saline (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... The PfPHB1-and PfPHB2-GST fusion proteins were purified via affinity chromatography from bacterial extracts using glutathione-sepharose (GE Healthcare, USA) using the soluble fractions of the lysed bacterial cultures ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in immunoprecipitation experiments with the anti-Cdc45 rabbit antibodies and control rabbit IgG bound to Protein A Sepharose beads (GE Healthcare). Mixtures were incubated for a minimum of 3 hr (or overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... We eluted bound proteins using an imidazole gradient and subjected them to size-exclusion chromatography (Superdex 200 16/600, GE Healthcare) using 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant recovered after centrifugation was used to purify proteins with GE HisTrap HP column (GE Healthcare Bio-Sciences, Piscataway, NJ) and a Bio-Rad EconoPump (Bio-Rad ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The sample was centrifuged after incubation with TEV and the supernatant containing the cleaved protein was purified by anion exchange chromatography using a column packed with either Q Sepharose High Performance (GE Healthcare) for Pex13 and 14 IDRs or SP Sepharose High Performance (GE Healthcare ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... and equal amounts of protein were separated on 5–20% SDS‒PAGE gels (Wako) and transferred to Hybond-P membranes (GE Healthcare). The following primary antibodies and dilutions were utilized ...
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bioRxiv - Plant Biology 2024Quote: ... Co-precipitation of StCWC15a/b with IPI-O1 was examined by protein blotting before (input) and after affinity purification (pull-down) using glutathione agarose beads (GE Healthcare). Interactions were detected using anti-GST and anti-His (Abmart).
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bioRxiv - Molecular Biology 2024Quote: ... The TEV-cleaved protein was recovered by second immobilised metal affinity chromatography (IMAC) and further purified by SEC (S200 16/60 column, GE Healthcare) in SEC buffer under isocratic flow ...
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bioRxiv - Biochemistry 2024Quote: ... protein was again passed over Nickel resin to remove His-tagged protein and further purified by size exclusion chromatography (Superdex 75 10/300g, GE healthcare) into 20mM Tris.HCl 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... These eluted proteins were subsequently passed through a superdex s75 16/60 column attached to an AKTA Pure system (GE healthcare) in 50 mM Tris pH 8.0 and 300 mM NaCl.
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bioRxiv - Molecular Biology 2024Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Crescendo and Forte Reagents and Amersham Imager 600 (GE Healthcare). Quantitative densitometric analyses were performed using ImageJ software.
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bioRxiv - Molecular Biology 2024Quote: ... Concentrated FhES (10 mg total protein) was fractionated (2 mL/fraction) by SEC on a Sephacryl S-200 HR 26/60 column (GE Healthcare) connected to an AKTA Start automated liquid chromatography system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... except that total extracts were subjected to pull-down using monoclonal antibodies against Cherry bound to protein G-Sepharose 4 Fast Flow beads (GE Healthcare). The suspensions were end-over-end mixed on a rotating wheel for 2 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The lysate underwent a subsequent centrifugation at 20,000 □g for 45 □min and the cleared soluble protein lysate was loaded on a prepacked Ni-NTA column (GE Healthcare). Proteins binding to the Ni-NTA coloumns were washed with 20 column volumes of binding buffer supplemented with 20 □mM imidazole to eliminate impurities ...
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bioRxiv - Microbiology 2024Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2024Quote: ... protein was further purified by size exclusion chromatography on a HiPrepTM 26/60 SephacrylTM S200 HR gel filtration column (GE Healthcare). Protein was eluted isocratically at a flow of 1 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...