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Citations for GE Life Sciences :
3551 - 3600 of 4126 citations for Japanese Encephalitis Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: The green state of PhoCl1 and PhoCl1 empty barrel recombinant proteins were further purified with size exclusion chromatography using SD200 column (GE Healthcare) and buffer exchanged to 20 mM Tris pH 7.4 ...
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bioRxiv - Plant Biology 2020Quote: ... an additional anion exchange (AIEX) step was performed prior to SEC by passing the protein over a 1 mL AIEX column (GE Healthcare). SnTox3 protein ...
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bioRxiv - Molecular Biology 2021Quote: ... total protein (50 μg) was subjected to SDS-PAGE followed by transfer to polyvinylidene difluoride membrane (GE Healthcare Life Sciences, Europe) using a semidry trans-blotter (BioRad ...
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bioRxiv - Biochemistry 2020Quote: SEC-MALS analysis was performed by injecting 100 μl of purified protein at 1 mg/mL onto a Superdex 200 10/300 Increase GL column (GE Healthcare), pre-equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and the soluble His6-M protein was purified on an Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with 5 μl rabbit polyclonal anti-GM130 serum or 5 μl pre-immune serum for 60 min at 4°C and incubated with protein A-Sepharose (GE Healthcare) for additional 60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing target protein were pooled and exchanged back to 100% buffer A using a Sephadex desalting column (HiPrep 26/10 column, GE Healthcare). TEV cleavage was performed overnight at 23°C with 1 mM DTT ...
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bioRxiv - Immunology 2021Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Biophysics 2020Quote: ... The protein in the supernatant was collected and subjected to gel filtration chromatography in a Superose6 10/300 GL column (GE Healthcare) previously equilibrated in Buffer B1 with 0.02% DDM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Molecular Biology 2021Quote: The binding affinity between the purified hPIV3 L-P complex protein and a RNA duplex were measured at room temperature using a Biacore S200 system (GE Healthcare) with a SA chip (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-protein binding complex was resolved on SDS-PAGE prior to analysis by Coomassie staining and PhosphoImaging (Typhoon FLA 7000, GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The protein binding complex was separated on SDS-PAGE prior to analysis by Coomassie staining and Phosphoimaging (Typhoon FLA 7000, GE Healthcare). For pull down with purified PGC-1α protein domains ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was monitored by Coomassie staining of SDS-PAGE and size exclusion chromatography (Superdex 75 10/300 GL, GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: 100 μl of protein sample was subjected to SEC at 293 K on a Superdex 200 (10/300) column (GE Healthcare) preequilibrated in 20 mM HEPES pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Simultaneously 3 μg of the anti-FLAG antibody (described above) was subjected to incubation with protein-G sepharose beads (60 ul, 17061801, GE Healthcare) for 1 hour with tumbling ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation was performed with the indicated primary antibodies and 50 μl of a 50% slurry of protein-A sepharose beads (GE Healthcare) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... the 6x His tag was cut off by the TEV protease and the protein was further purified by size-exclusion chromatography on a Superdex 75 HiLoad 26/60 column (GE Healthcare) in SEC buffer (20 mM Na2HPO4 ...
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bioRxiv - Zoology 2020Quote: ... Proteins were separated in the second dimension on a 14% SDS polyacrylamide gel (Laemmli, 1970) in a Hoefer600 cell (GE Healthcare) for 15 min at 15 mA (100 V/15 W limits ...
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bioRxiv - Immunology 2021Quote: ... was used to elute the protein using a binding buffer with 400 mM imidazole followed by size exclusion chromatography on a Superdex75 16/600 (GE Healthcare) column equilibrated in binding buffer ...
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Immunology 2020Quote: ... The soluble xfR5 mAb from the supernatant was isolated using HiTrap™ Protein-A HP prepacked column (GE Healthcare; NJ, USA) following standard manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed using a French press and recombinant proteins were purified by nickel ion affinity chromatography (HisTrap HP, 1ml - GE Healthcare). The His6-SUMO tag was removed using Ulp1 protease (Li and Hochstrasser ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biochemistry 2019Quote: ... Samples (100 μl) ranging in protein concentration from 15-250 μM were applied to a Superdex 200 10/300 GL column (GE Healthcare) mounted on a Jasco HPLC equilibrated in 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... All Rab proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Non-specifically bound proteins were removed by a step wash with buffer B (50 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... Protein samples (each protein at a concentration of 40 μM) were loaded to a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with assay buffer (same with ITC).
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bioRxiv - Molecular Biology 2019Quote: ... Both the cleaved His-tag and the His-tagged protease were removed from protein solutions by passage over loose nickel beads (GE Healthcare). The tag-free NDC-NDC was further purified with HiTrap SP HP ion exchange column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... Dialyzed protein was diluted two-fold in buffer Q1 and loaded onto a HiTrap Q HP anion exchange chromatography column (GE Healthcare), equilibrated in buffer Q1 ...
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bioRxiv - Biochemistry 2020Quote: ... Cell extract was clarified by ultracentrifugation at 140 000 x g at 4 °C for 45 min and recombinant CNOT7 protein was purified automatically using ÄKTAxpress FPLC system (GE Healthcare). The following protocol was used for automated purification ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were expressed transiently in HEK-293T cells and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare) or streptactin beads (IBA ...
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Diafiltrated cell supernatant was purified by immobilized nickel affinity chromatography (5-ml fast flow crude column and ÄKTA fast protein liquid chromatography [FPLC] system; GE Healthcare) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The membranes were washed three times with TBS-T and protein bands were visualized using the CDP-star detection reagent (GE Healthcare). Intensity was detected and quantitatively analyzed by the Fluor-S MAX multi-imager system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with 240 mM imidazole and further purified over a Superdex S-75 gel filtration column (GE Healthcare) equilibrated in 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Pooled fractions of protein were then concentrated and loaded onto a 10/300 Superdex200 Increase gel filtration column (GE Life Sciences) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... the TTPN protein was concentrated and loaded on a size-exclusion chromatography (SEC) column (HiLoad 26/60 Superdex 75 pg, GE Healthcare). Peak fractions from the first SEC step ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... fused to lysozyme and BRD4-ET either alone or together was evaluated by injecting each purified protein onto a Superdex 75 Increase 10/300 GL Increase column (GE Healthcare) equilibrated with 20 mM HEPES 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The fractions containing Mre11 protein were concentrated on a 50 kDa MWCO Amicon concentrator and applied onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in Mre11 SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein containing elution fractions were concentrated on Amicon concentrator (100 kDa MWCO) and loaded a Superose 6 16/600 (GE Healthcare) pre-equilibrated in SEC buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein bands were visualised using ECL™ or ECL plus™ reagents and Hyperfilm® ECL (all from GE Healthcare) and detected with a CCD camera (Biorad) ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated on a 10% polyacrylamide gel and transferred to a nitrocellulose membrane (GE life sciences, 1060002) using a Pierce™ Power Station (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
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bioRxiv - Biophysics 2021Quote: ... was purified by 1 M NaCl containing Tris buffer using AKTATM Pure protein purification system with Hi-Trap Q-FF anion exchange column (GE healthcare). 0.3% H2O2 was added to the purified proteins ...
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bioRxiv - Immunology 2022Quote: SEC-MALS experiments were carried out using a Wyatt Dawn Heleos II multiangle light scattering detector (Wyatt Technology) coupled to an AKTA Purifier UPC10 FPLC protein purification system and a Superdex 200 size exclusion column (GE Healthcare). Protein molecular weights of the individual peaks observed in the size-exclusion chromatograms were analyzed by static light scattering (SLS ...