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Citations for GE Life Sciences :
3451 - 3500 of 4126 citations for Japanese Encephalitis Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... After incubation with primary antibodies overnight at 4°C followed by the addition of Protein A or G Sepharose beads (GE Healthcare) for 2-3 hrs ...
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bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Biochemistry 2022Quote: ... Salt concentration was adjusted to 250 mM KCl and the protein was loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.
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bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was purified with subtractive Ni-NTA followed by purification on a Superdex 75 16/600 GL column (GE Healthcare), in a size exclusion buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... tagging of the protein was visualised by running an SDS PAGE followed by scanning in a Typhoon scanner (GE Healthcare, Sweden).
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bioRxiv - Biochemistry 2022Quote: All chromatography purification steps were carried out at 4°C using an ÄKTA pure fast protein liquid chromatography (FPLC) system (GE Healthcare). All buffer solutions were chilled prior to use ...
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bioRxiv - Molecular Biology 2022Quote: ... immobilized protein was eluted using wash buffer and further loaded onto an ion-exchange column (MonoQ 5/50 GL column, GE Healthcare) to achieve highly pure PBAF complex ...
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bioRxiv - Synthetic Biology 2022Quote: Protein cargo exchange experiments were performed by incubating 100 nM DNA origami with 300nM Alexa-647 labelled protein cargo for 1h before addition to washed s300 resin (according to manufacturer’s instructions, GE Life Sciences) in a spin column and centrifugation for 4 mins at 1000 g to remove free protein ...
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bioRxiv - Microbiology 2022Quote: ... The final protein purification step and determination of oligomeric state was done on a Superose6 Increase 10/300 size exclusion column (GE Healthcare). The column was equilibrated in buffer B and the concentrated protein was loaded onto the column ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2022Quote: ... The former vector encodes a glutathione S-transferase (GST) fusion protein that can be removed proteolytically with Prescission Protease (GE Healthcare) after affinity purification ...
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bioRxiv - Microbiology 2022Quote: ... Cells that integrated the fluorescent marker were selected on TSA with 15 µg/mL gentamycin and confirmed for their ability to expression the mCherry fluorescent protein using a Typhoon FLA 9000 imaging system (GE Healthcare). The gentamicin resistance cassette was flipped by electrotransformation of the pFLP3a plasmid containing the FLP-recombinase into the newly produced fluorescent cells.
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bioRxiv - Immunology 2022Quote: ... The antibodies were purified from Expi293 cell supernatant by high-performance liquid chromatography (HPLC) coupled with HiTrap Protein A HP columns (Cytiva, formerly GE Healthcare). Monoclonal antibodies were then dialyzed by SnakeSkin dialysis tubing (10K MWCO ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Biochemistry 2022Quote: ... SEC was carried out on a Superose 6 Increase 10/300 GL column by fast protein liquid chromatography (ÄKTA; GE Healthcare); 0.25 mM fractions were collected ...
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bioRxiv - Biophysics 2022Quote: ... Protein concentration of the extracts were determined by SDS-PAGE and densitometry analysis using the LMW-SDS Marker Kit (GE Healthcare) as standard.
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bioRxiv - Cell Biology 2022Quote: ... 500 µg of the purified protein or the complex was loaded on a Superdex 200 10/300 size exclusion column (GE Healthcare) in line with both a DAWN HELIOS II MALS detector (Wyatt Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were separated by SDS–PAGE and transferred to a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences, Marlborough, USA); the membranes were blocked with 1% nonfat milk powder and incubated together with primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Signals corresponding to protein levels were detected using Amersham ECL Prime detection reagent and an Amersham Imager 600 instrument (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... and mAb was purified from spent medium by affinity chromatography on an ÄKTATM pure Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated on 10 % acrylamide gels by SDS-PAGE and protein transferred to a 0.45 μm PVDF membrane (GE Healthcare 15259894) using the BioRad TransBlot Turbo system ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 µL of supernatants were stored as input and the rest were incubated with the lysis buffer-prewashed G-sepharose beads (50 µL of 50% slurry per each reaction; Protein G-Sepharose 4 Fast Flow, GE Healthcare) for 3 h at 4 °C while rotating ...
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bioRxiv - Plant Biology 2021Quote: ... The cells were grown 1 day at 28 °C and two days at 22 °C at 110 rpm The secreted proteins were purified separately by sequential Ni2+ (HisTrap excel, GE Healthcare, equilibrated in 25 mM KPi pH 7.8 and 500 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... normalized protein amounts were resolved under non-reducing conditions by SDS–PAGE and blotted to Hybond-P PVDF membrane (GE Healthcare) for 1 hour at 30 V ...
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bioRxiv - Immunology 2021Quote: ... Affinity-purified proteins were then purified by size exclusion chromatography using a HiLoad 26/600 Superdex 200 pg column (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... was separated from the remaining tag protein and protease by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). The unbound tag-free protein was collected and protein concentration was determined by absorbance (A280 ...
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bioRxiv - Microbiology 2021Quote: ... The final purification of CwlQ-6His protein was conducted via size exclusion chromatography on a Superdex 75 16/60 (GE Healthcare) column using gel filtration buffer (25 mM Tris [pH 8.0] ...
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bioRxiv - Plant Biology 2021Quote: ... the eluates containing recombinant proteins with 10× His-MsyB tag were further purified by an anion-exchange column (Source-15Q; GE Healthcare). The 10×His-MsyB tag was cleaved by TEV protease at 4 °C overnight and removed by an anion-exchange column ...
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bioRxiv - Microbiology 2020Quote: ... The concentrated protein was then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare, HiLoad 16/60) equilibrated with buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2021Quote: The scCl5 was purified using a two-steps protocol using first immobilized metal affinity chromatography (IMAC) to enrich for His tagged protein (1 ml HisTrap, GE Healthcare) followed by anion exchange (1 ml HiTrapQ ...
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bioRxiv - Microbiology 2020Quote: ... amino acids 319-537) (Acro Biosystems, Newark, NJ) protein was measured at 25°C using BIAcore T200 surface plasmon resonance (SPR) (GE Healthcare). STI-1499 or STI-2020 antibody was covalently immobilized on a CM5 sensor chip to approximately 500 and 100 resonance units (RU) ...
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bioRxiv - Microbiology 2020Quote: ... the VHH-72-Fc protein in the cleared cell culture medium was captured on a 5 mL MabSelect SuRe column (GE Healthcare), eluted with a McIlvaine buffer pH 3 ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified to homogeneity by protein A affinity chromatography and characterized by size exclusion chromatography on a Äkta Pure FPLC system (GE Healthcare) with a Superdex S200 10/300 increase column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: The native molecular mass of the recombinant proteins was determined by gel filtration using a Superdex 200 10/300 column (GE Healthcare), previously calibrated with protein standards (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified by gel filtration chromatography on a HiLoad™ 16/600 Superdex 200 pg column (GE Healthcare) with the following buffer ...
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bioRxiv - Immunology 2020Quote: ... Ab binding (protein expression) was visualized using Amersham ECL Prime Western blotting detection reagent (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). The following Abs were used in this study ...
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bioRxiv - Neuroscience 2021Quote: ... The concentrated protein was subjected to size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare, Chicago, IL) run in Buffer 3 (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Proteins were concentrated and subjected to size-exclusion chromatography on a HiLoad 26/600 Superdex 200 pg (GE Healthcare; Cat# 28989336). Trimer-containing fractions were pooled ...
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bioRxiv - Immunology 2021Quote: ... The recombinant proteins were purified from supernatants by affinity chromatography with a Ni-agarose column (HisTrapTM HP, GE Healthcare, Chicago, IL), dialyzed against PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The RBD proteins were further purified with size-exclusion chromatography using a Superdex 200 Increasse 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: The binding affinities of full IgG antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Biophysics 2020Quote: ... The flow-through that contained the protein of interest was then applied to a HiTrap Q 5 mL column (GE Healthcare). The Q column flow-through containing nsp8 or nsp7 was concentrated using a MWCO 3 kDa Amicon Ultra Centrifugal Filter (Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... protein extracts were resolved on SDS-PAGE gels under denaturing conditions and transferred to Hybond ECL membranes (Amersham Biosciences, GE Healthcare). Membranes were blocked for 1 hour at RT and incubated overnight with the respective primary antibody (Supplementary Table S2 ...
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bioRxiv - Microbiology 2021Quote: GST alone and GST-tagged WWOX WW1 and WW2 domains fusion protein were expressed in BL-21 cells and subsequently purified and conjugated to glutathione (GSH) beads (GE HEALTHCARE). HEK293T cells were transfected with eVP40-WT ...
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bioRxiv - Immunology 2021Quote: The purification of S protein from cell broth was performed using on AKTA Pilot 600R system (GE Healthcare, Little Chalfont, UK). After harvesting ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...