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Citations for GE Life Sciences :
3551 - 3600 of 4416 citations for Dengue Virus Serotype 3 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: IgG was purified from plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the protein was further purified by size exclusion chromatography using a Superdex 200 Increase 100/300 GL column (GE Healthcare). The purified scFv16 were pooled ...
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bioRxiv - Plant Biology 2023Quote: Protein samples were resolved in a 12% SDS-polyacrylamide gel electrophoresis (PAGE) and then transferred to nitrocellulose membranes (GE Healthcare), blocked with 5% skimmed milk in TBST (Tris-buffered saline ...
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bioRxiv - Cell Biology 2023Quote: ... and further purified by affinity chromatography on an affinity column containing the above mentioned connexin36 segment protein coupled to CNBr-activated Sepharose (14 mg to 2.5 g Sepharose 4B; GE Healthcare). The specificity of the affinity purified antibodies was tested on tissue extracts and cryostat sections (12 µm thick ...
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bioRxiv - Microbiology 2023Quote: ... His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare) in 50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The protein monomers were separated from oligomers by size exclusion chromatography on a Superdex 200 column (120 mL, GE Healthcare) equilibrated with buffer containing 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... the protein sample was loaded into a HiTrapTm Q HP anion exchange column (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) and eluted with ∼ 50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was finally purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) with buffer E and concentrated to 10 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 4°C at 10,000 g for 30 minutes and the supernatants were used to purify target proteins by Ni NTA agarose (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were further purified via size exclusion chromatography using a GE Superdex200 Increase 10/300 GL column (GE Healthcare) in running buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... then separated by fast protein liquid chromatography system using HiTrap SP HP column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: Protein molecular mass at the native state was determined by gel filtration using a Superdex200 10/300 column (GE Healthcare). A gel filtration calibration kit (high molecular weight ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins (30 µg/lane) were resolved by SDS-PAGE and electro-transferred to the Hybond-P PVDF membranes (GE Healthcare). Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction solution was sequentially precipitated using protein A sepharose beads and Glutathione Sepharose beads (#17-0756-01, GE Healthcare). The resulting supernatant was stored at −20 °C until it was analyzed for the surface plasmon resonance (SPR ...
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bioRxiv - Microbiology 2023Quote: ... The protein sample was further purified by FPLC using a Superose® 6 Increase 10/300 GL column (GE Healthcare) equilibrated in sizing buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was finally purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare), pre-equilibrated in SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the protein of interest were applied to a Sephacryl 300 16/60 gel filtration column (GE Healthcare) and eluted with buffer D (10 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The small-scale reconstitution reactions were pooled after dilution and concentrated using a 100kDa MWCO Vivaspin protein concentrator (GE Healthcare). The sample was subjected to size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2023Quote: ... The untagged protein was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare), eluted in buffer E (25 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... proteins from extracts were separated on SDS polyacrylamide gels and blotted onto Nitrocellulose membrane (Hybond ECL Cat#RPN2020D, GE Healthcare). Membranes were blocked using Skimmed Milk 5% ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitated proteins were separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane (Amersham Protran, GE Healthcare Life Sciences) using a wet transfer method ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and proteins were transferred to Cytiva Amersham™ Protran™ 0.45 µm NC nitrocellulose blotting membranes (GE Healthcare, Munich, Germany). After blocking with 5% bovine serum albumin (Roth ...
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bioRxiv - Immunology 2024Quote: ... The complexes of SH-01AF594 with C3d and Fab 3E12 were obtained by concentrating ∼1 mg of either protein with 300µg of SH-01AF594 to ∼200µL before removing the excess protein via SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare), pre-equilibrated in HBS ...
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bioRxiv - Biochemistry 2024Quote: ... with the exception that the protein eluted from the Ni-NTA column was loaded on HiTrap Heparin column (GE Healthcare) equilibrated with HP1 buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was concentrated and loaded onto a S75 16/600 size exclusion chromatography column (GE Healthcare, Chicago, USA). After chromatography ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of protein was immobilized on a nitrocellulose membrane (Amersham) by filtration using a slot blot apparatus (GE Healthcare). The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by sodium dodecylsulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and transferred onto 0,45 μm nitrocellulose membranes (GE Healthcare) which were then probed with the following primary and secondary antibodies with the indicated dilutions ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... and proteins were collected from culture supernatants and purified using the HisTrap HP column (GE Healthcare Life Sciences, Pittsburgh, PA) as described previously 33 ...
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bioRxiv - Microbiology 2024Quote: ... the labeled proteins were verified by running an SDS-PAGE and scanning using a Typhoon scanner (GE Healthcare, Danderyd, Sweden).
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bioRxiv - Plant Biology 2020Quote: ... Fifty micrograms of protein were separated on an SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE healthcare). The rabbit polyclonal antibody to PHO1 [51] and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the protein was then purified by size exclusion chromatography (HiLoad 16/600 Superdex 75 pg column, GE Life Sciences cat#28989333). Purified protein was concentrated using Amicon Ulta 10k M.W ...
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Biophysics 2021Quote: ... thrombin was removed and the proteins were concentrated by ion exchange chromatography on MonoQ or MonoS columns (Äkta purifying system, GE Healthcare). The Δ49-complex was assembled by first mixing syx-1 and the soluble syb 2 fragment (1:2 ...
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bioRxiv - Biophysics 2021Quote: ... the concentrated protein sample was loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (GE Healthcare Life Sciences) and eluted in the gel-filtration (GF ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing the eluted and cleaved protein was concentrated down and applied onto a Superdex 75 column (16/60 prep grade, GE Healthcare) and eluted with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Biophysics 2019Quote: ... The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified over a HisTrap FF nickel affinity column followed by Superdex 75 size exclusion chromatography using the ÄKTA protein purifier purification system (GE Healthcare), as previously described (27) ...
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...