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Citations for GE Life Sciences :
3801 - 3850 of 4416 citations for Dengue Virus Serotype 3 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were detected by fluorescence (Odyssey, Li-Cor) or revealed using the ECL Prime Western Blotting Detection Reagent kit (GE Healthcare) and images were acquired in a dark room using a G:Box (Syngene ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were further purified by gel filtration chromatography using a Superdex 200 column attached to an AKTA-format FPLC (GE Healthcare) and running buffer containing 50mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was run over a 10/300 Superose 6 size exclusion column (GE Life Sciences, cat#17–5172–01). Fractions containing the trimeric F proteins were pooled and concentrated by centrifugation in a 50 kDa Amicon ultrafiltration unit (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... size-exclusion chromatography of all proteins was carried out on either HiLoad 26/60 or 16/600 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... GST- DIDATRX fragments and His-DIDATRX proteins were purified directly after Ni-NTA using HiLoad 16/60 superdex 200 or 75 size exclusion columns (GE Healthcare). With the exception of proteins used in NMR experiments ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 12 % denaturing acrylamide gel and subsequently transferred onto a nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membrane was blocked using 1x Roti®Block (Carl Roth ...
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bioRxiv - Developmental Biology 2022Quote: ... The media was buffer exchanged with PBS and His-tagged proteins were captured with 5 mL HisTrap Excel columns (GE Healthcare), washed with 20 mM imidazole and eluted with 300 mM imidazole containing PBS buffer ...
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bioRxiv - Biochemistry 2022Quote: ... 3C protease was removed by Ni-chelated sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... TEV and residual affinity tag was removed by passing the protein through a Superdex 200 Increase 10/300 GL (GE Healthcare) in SEC buffer (30 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Biochemistry 2019Quote: ... The proteins were then subjected to size-exclusion chromatography using a HiLoad 16/60 Superdex 200 prep grade column (GE Healthcare) equilibrated in SEC buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts of GST or GST-fusion proteins were incubated for 0.5-1h with glutathione-coated sepharose beads (GE Healthcare, Freiburg, Germany) equilibrated in PBS (Boi1-Bni1 ...
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bioRxiv - Biochemistry 2020Quote: ... The fluorophores and other small molecules were removed from the proteins by passing the reaction mixture through a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with the Protein Buffer ...
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bioRxiv - Biochemistry 2019Quote: ... the TNC buffer in which proteins were stored was exchanged using Sephadex G-25M PD-10 desalting gravity columns (GE Healthcare) into 50 mM N-Cyclohexyl-2-aminoethanesulfonic acid (CHES ...
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bioRxiv - Neuroscience 2019Quote: SPR biosensor analysis was used to verify binding between peptides and the PDZ2 domain of FRMPD2 protein using a Biacore 8K instrument (GE Healthcare), as previously described(Zhang et al. ...
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bioRxiv - Physiology 2020Quote: ... The labeled protein was subsequently purified using size exclusion chromatography on a Sephadex™ G-25 Medium column (GE Healthcare, UK). To remove excess free dyes from the labeled proteins and also avoid hydrolysis of extra fluorophore ...
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bioRxiv - Immunology 2019Quote: ... Analytical size exclusion chromatography showed that the majority of the protein eluted in a peak at 13 mL from a Superdex 200 column (GE Healthcare). BacILT7 was dialysed against HBS-PE and cleared by centrifugation at 100,000 g for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... The components were ran electrophoretically and the proteins were electrotransferred to a polyvinylidene difluoride membrane (Hybond-P™, Amersham GE Healthcare) at 50 V and a current of 90 mA overnight using a transfer buffer (1.9% Tris base ...
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bioRxiv - Biochemistry 2019Quote: ... with expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Biophysics 2019Quote: ... The eluted protein fraction was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) in the buffer of 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2019Quote: ... SMCHD1 or γH2AX) and 30µL of preblocked Protein G Sepharose 4 Fast Flow 50% bead slurry (GE Healthcare, #17-0618-01) overnight at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified using reverse IMAC with the cleaved protein being loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Zoology 2019Quote: The midgut BBMV proteins were subjected to clean-up using the 2-D Clean-up Kit (GE Healthcare Life Sciences, USA) as per the manufacturer’s instructions ...
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bioRxiv - Zoology 2019Quote: ... The rehydrated IPG strips containing 100 μg of proteins were then subjected to isoelectric focusing as per the manufacturer’s standard protocol (GE Healthcare, USA). After IEF ...
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bioRxiv - Biophysics 2020Quote: ... and the protein was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), equilibrated with buffer E (20 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... and the protein was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare), equilibrated with buffer B ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted proteins were analyzed in an 8% SDS-polyacrylamide gel and scanned and quantitated using a Typhoon 9400 laser imager (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... Afterwards the immobilized papain resin was removed and the digested fraction was mixed with Protein A Sepharose (0.2 ml resin for 1 mg digested IgG, GE Healthcare, #17078001) for 1 hour at RT ...
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bioRxiv - Microbiology 2019Quote: ... the His6-tag was removed using TEV protease and the protein was subjected to size exclusion chromatography (HiPrep 26/60 Sephacryl S-200; GE Healthcare) using a buffer consisting of 40 mM HEPES pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... For the in vitro ATPase activity assay and pull-down assay the buffer of the proteins was exchanged via a PD SpinTrap G-25 spin columns (GE Healthcare) to activity assay buffer (25mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting SNX9 and His6x-β2-hinge+appendage proteins were separately applied to a HiLoad 26/600 Superdex 200 pg column (GE Healthcare). Peak fractions of target proteins were collected and concentrated using Amicon Ultra-15 10K Centrifugal filters (Sigma-Aldrich).
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bioRxiv - Biochemistry 2019Quote: ... the dialyzed protein was filtered and loaded on a cation exchange HiTrap SP HP 5 ml column (GE healthcare life sciences) pre-equilibrated with 5 column volumes of buffer ASP (100 mM Ammonium acetate ...
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bioRxiv - Biochemistry 2019Quote: Nucleotide concentrations of an ongoing enzymatic reaction were determined in real-time by fast protein liquid chromatography (FPLC) using an ÄKTA Purifier instrument (GE Healthcare) equipped with a 1 mL Resource Q anion exchange (AEX ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to the protein solution to a final concentration of 1.3 μM and incubated for 15 minutes at room temperature in order to cleave off His10-SUMO tag and the protein solution was applied on HisTrap 1 HP (GE Healthcare) loaded with Zn2+ and pre-equilibrated with storage buffer ...
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bioRxiv - Immunology 2019Quote: ... ERV-K envelope protein was cloned out of the sequencing vector and ligated into pGEX-6p-1 N’ GST-tag expression vector (GE Healthcare) between EcoRI and NotI sites using the following primers:
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bioRxiv - Cell Biology 2019Quote: ... phenolics and nucleic acids in extracted protein were removed using the 2-D Clean-Up kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitation performed from whole cell extracts of NIH3T3-II10 and NIH3T3-K2 cells were following the same procedures with protein G Sepharose beads (GE Healthcare): 18 μg rabbit anti-TBPL2 (3024 ...
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bioRxiv - Microbiology 2020Quote: ... The protein in the supernatant was sequentially purified using a Ni-NTA column and a Superdex200 gel filtration column (GE Healthcare) as previously described (20) ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were further purified by size exclusion chromatography (SEC) on a Superdex 75 Increase 10/300 GL column (GE Healthcare) for E2 cores and on a Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... Cleaved protein was eluted from the beads and further purified on a cation exchange column (Mono S 10/100, GE Healthcare) in MES pH 6.5 ...
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bioRxiv - Bioengineering 2019Quote: ... 600 µL of a 1:10 dilution of serum was applied onto the protein G column (Ab Spin trap; GE Healthcare) and washed ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated with rotation at 4°C first for 45 min with antibodies to FLAG and then for 45 min with protein A–Sepharose (GE Healthcare). The resulting immunoprecipitates were subjected to immunoblot analysis.
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... Cross-linked proteins were further purified from aggregation products by size exclusion chromatography on a Superose 6 increase column (GE Healthcare) equilibrated with size exclusion buffer (10 mM HEPES pH 7.8 ...
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to remove remaining fluorophores and aggregates ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates with equal total protein amount were incubated with 50 μl pre-washed Glutathione Sepharose 4B (GE Healthcare Life Sciences) in 100 μl GST pull-down buffer (20 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... Protein eluates were then concentrated and buffer exchanged to PBS using Vivaspin filters with the appropriate molecular weight cutoff (GE Healthcare). Protein concentrations were determined by the Nanodrop method using the proteins peptidic molecular weight and extinction coefficient as determined by the online ExPASy software (ProtParam).
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bioRxiv - Synthetic Biology 2020Quote: The green state of PhoCl1 and PhoCl1 empty barrel recombinant proteins were further purified with size exclusion chromatography using SD200 column (GE Healthcare) and buffer exchanged to 20 mM Tris pH 7.4 ...