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Citations for GE Life Sciences :
3501 - 3550 of 3899 citations for 2' 3' cGAMP STING based FRET Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Cell debris was removed by centrifugation at 10000xg for 45 min at 4°C and the supernatant was loaded onto either a 1 mL or 5 mL HisTrap FF crude column (GE Healthcare) equilibrated in Ni-1 buffer using a peristaltic pump ...
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bioRxiv - Molecular Biology 2019Quote: ... washed 3x with TBS-T and incubated for 1 hours at RT with the secondary antibody (horseradish peroxidase-conjugated sheep anti-mouse or donkey anti-rabbit IgG, GE Healthcare). Membranes were analyzed with a Fusion Solo (Vilber Lourmat) ...
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bioRxiv - Cell Biology 2019Quote: ... Series of 3D z-stacks were acquired with 0.5 μm spacing every 1 min for 15 min using an Olympus DeltaVision wide-field microscope (GE Healthcare, Switzerland) equipped with a eGFP/RFP filter set (Chroma ...
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bioRxiv - Physiology 2019Quote: ... and 1 min effluent samples were collected from the venous cannula using a fraction collector (Frac-920; GE Healthcare, Brøndby, Denmark) and stored at −20°C until analysis ...
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bioRxiv - Biochemistry 2019Quote: Human BRG1 AT-BD (residues 1434–1569) or BD (residues 1454-1569) were cloned into a pGEX6p-1 vector (GE Healthcare) from full length BRG1 cDNA obtained from GE Open Biosytems ...
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bioRxiv - Biochemistry 2019Quote: ... The labeling reaction was quenched after 1 hr with fresh DTT at 100 mM final concentration and loaded onto a HiPrep 26/10 desalting column (GE Healthcare). The final labeled proteins were concentrated to at least 80 µM and flash-frozen in Protein Buffer (50 mM Tris-HCl [pH 7.2] ...
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bioRxiv - Microbiology 2019Quote: ... plaque assays were washed with PBS three times and then incubated for 1 h in anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Biochemistry 2019Quote: The rate of alarmone synthesis was determined by quantifying AMP levels at set time-points using anion exchange chromatography (on a 1 ml Mono Q 5/50 GL column (GE Healthcare), as described above) ...
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bioRxiv - Biochemistry 2019Quote: ... The HiTrap ButylFF column of 1 mL and the gel filtration column Superdex 200 10/300 GL were from GE Healthcare. MacroPrep HighS resin was from Biorad ...
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bioRxiv - Immunology 2020Quote: ... was accomplished using 0.5 M imidazole and fractionated into 1 ml aliquots using the Frac30 fraction collector (GE Life Sciences # 29023051). Fluorescence of FAST proteins was checked via brief excitation (Figure 2D ...
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bioRxiv - Genomics 2019Quote: ZHBTc4 YPet-MD TIR1-V5 mCherry-OCT4-AID cells were plated in a 96-well plate coated for 1 hour at 37°C with 1:25 diluted StemAdhere (Primorigen Biosciences #S2071-500UG) and imaged on an IN Cell Analyzer 2200 (GE Healthcare) using the TexasRed and Brightfield channels ...
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bioRxiv - Microbiology 2020Quote: ... followed by anti-mouse IgG horseradish peroxidase (HRP)-linked whole Ab sheep (1:5,000 dilution in PBS) (GE Healthcare Bio-Sciences), and reacted with 3 ...
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bioRxiv - Molecular Biology 2019Quote: ... The amplified fragments were digested with BamH I and Not I then subcloned into the same sites of pGEX- 6P-1 vector (GE Healthcare). Halo-tagged NIPBL mutants “499-1124 a.a.” and “1844-2804 a.a.” (Oka et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 12000 g at 4 °C for 1 h and loaded onto a HisTrap HP column (GE Healthcare) equilibrated with 98% buffer A/2% buffer B (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was stopped by the addition of 1 mM PMSF and the proteolyzed complex was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Genetics 2021Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Rate of cleavage (nucleotide/minutes ...
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bioRxiv - Microbiology 2021Quote: ... ParBΔCTD-containing fractions were pooled and diluted to a conductivity of 16 mS/cm before being loaded onto a 1-mL Heparin HP column (GE Healthcare) that had been pre-equilibrated with 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... and EBV BFLF2 residues 78 to 318 (Uniprot entry: K9UT32) were each cloned into a pGEX-6P-1 vector (GE Healthcare). The constructs include an N-terminal GST affinity tag and a HRV 3C protease cleavage site (SDLEVLFQGPLGS) ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction mixture was incubated at 37°C for 1 h and labeled EVs purified using a PD-10 size exclusion chromatography column (GE Healthcare). Instant thin layer chromatography (iTLC ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Biochemistry 2021Quote: ... LPMO containing fractions were pooled and concentrated to 1 mL before being loaded onto a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) operated with a running buffer consisting of 50 mM Tris pH 7.5 and 200 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were purified by loading the supernatant onto a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) equilibrated with 5 CV of 95% Buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare), which was equilibrated with MonoQ buffer A containing 50 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... primary mouse hepatocytes isolated via the two-step collagenase perfusion method were suspended in 1 ml DMEM media and subjected to Percoll (GE Healthcare) gradient centrifugation at 1,000g for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM MgCl2 1 mM DTT) and further purified by size exclusion chromatography on a pre-equilibrated Superdex 200 column (GE healthcare). Protein in size exclusion buffer was concentrated to ∼10 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was diluted two-fold in buffer E + 1 mM DTT + 150 mM NaCl and applied to a MonoS column (GE Healthcare) pre-equilibrated in buffer E + 150 mM NaCl + 1 mM DTT + 0.5 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated to less than 1 mL and was injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 293-F cells were seeded 2-3 h before transfection at a density of 1×106 cells per ml in Freestyle medium supplemented with 0.2% w/v HyClone Cell Boost (GE Healthcare, SH30584.02). For transient transfection ...
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved KIX was purified using a linear gradient of 50 mM to 1 M NaCl over ten column volumes by cation exchange chromatography using MonoS 5/50 GL column at a flow rate of 1 mL per minute (GE Healthcare). Fractions containing purified KIX were confirmed by SDS-PAGE and dialyzed against 1 L of 50 mM potassium phosphate pH 5.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was next loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was then loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: Total RNA (10 µg) was electrophoresed on a 1% formaldehyde denaturing gel and transferred onto the Hybond N+ membrane (GE Healthcare). The BrGypsy ...
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bioRxiv - Biochemistry 2021Quote: ... The dialyzed DCX sample was centrifuged to remove any precipitates before loading onto a Strep-Trap (1 mL, cat. No. 29-0486-53, GE Healthcare) column ...
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bioRxiv - Biochemistry 2020Quote: ... the concentrated fractions containing ɸEL (eluting at 8–15 mS cm-1) were subjected to size exclusion chromatography (SEC) on Sephacryl S-400 (GE Healthcare) in buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 10% (v/v) glycerol and 1 mM DTT prior to ion exchange chromatography using a Fast Flow SP column (GE Healthcare). Elution from the SPFF column was performed with a 100 mM to 1M gradient over 20 column volumes ...
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bioRxiv - Bioengineering 2021Quote: ... The biotinylation mixture was incubated at room temperature for 1 h and then loaded onto a HiPrep 26/10 desalting column (GE Healthcare) pre-equilibrated with a storage buffer (1×PBS ...
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bioRxiv - Cell Biology 2021Quote: Membranes were then probed with goat anti-rabbit HRP (1:10,000, Licor) before being developed with SuperSignal West Femto (GE Life Sciences) and imaged on an iBright1500 (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT followed by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h with 5 μL of anti-MYC antibody (Gentex) followed by 1 h incubation with an additional 50 μL of GammaBind plus Sepharose beads (GE Healthcare) to capture protein complexes ...
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 1470 bp) were purified and restricted with BamH1 and EcoR1 prior to subcloning into pGEX-6P-1 (GE Healthcare, Uppsala, Sweden) digested with the same enzymes to produce plasmids to produced N-terminally GST-tagged versions of NoAUREO 2 ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
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bioRxiv - Plant Biology 2021Quote: ... using an AKTA Pure 25 system equipped with HisTrap HP 1 mL column and submitted to gel filtration using a Superdex 200 increase 10/300 GL column (GE healthcare). Purified recombinant proteins were conserved at −80°C in 1x PBS solution containing 10% glycerol.
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... The buffer was then adjusted to contain 40 mM imidazole and the rNAs were purified with a HisTrapTM FF 1 ml column (GE Healthcare) using an Akta Start protein purification system (Cytiva) ...
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bioRxiv - Immunology 2020Quote: ... Membranes were washed 3 times with TBS-T and stained with 1:10 000 HRP-coupled anti-rabbit (anti-Rabbit IgG, HRP linked antibody from donkey, GE HealthCare) in blocking buffer for 1 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl) of lgG Sepharose beads (GE Healthcare) for 1 h at 4 °C ...