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Citations for GE Life Sciences :
3301 - 3350 of 3899 citations for 2' 3' cGAMP STING based FRET Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 1 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Microbiology 2022Quote: ... For all primary mouse antibodies an anti-mouse-HRP in a dilution of 1:2000 was used (GE Healthcare). All blots were blocked with TBS-T 5% Milk and washed 3x for 15 minutes with TBS-T after each antibody incubation ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... and then purified through affinity chromatography using a 1 mL HiTrap Protein G HP column (GE Healthcare Life Sciences). Antibodies were eluted from the column using 0.1 M glycine-HCl ...
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bioRxiv - Synthetic Biology 2022Quote: ... The filtered solution was then purified by Ni2+ affinity chromatography using a 1 mL HisTrap FF column (GE Healthcare). The column was equilibrated with 25 mL Buffer A at a constant flow rate of 1 mL/min ...
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bioRxiv - Biophysics 2024Quote: ... at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences, Marlborough MA) at a 1:10,000 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble extract was loaded onto the Hi-Trap Chelating HP 1 ml pre-packed column (GE Healthcare/Cytiva) pre-equilibrated with buffer A containing 500 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... was used at 1:10,000 and proteins were visualised by enhanced chemiluminescence using the ECL-Plus system (GE Healthcare).
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bioRxiv - Immunology 2024Quote: NP constructs (each at 100 μg) were loaded separately on 1 ml heparin column (GE17-0407-01, GE Healthcare) pre-equilibrated with buffer A (20mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated anti-rat or anti-mouse (1:2000; GE healthcare) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was filtered through 0.45 µm filters before loading onto a 1 mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A (100 mM Tris-HCl [pH8] ...
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bioRxiv - Neuroscience 2023Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein in the supernatant was diluted to 1% Triton and incubate with Glutathione Sepharose beads (GE Healthcare) in a batch mode ...
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bioRxiv - Molecular Biology 2023Quote: ... washed in TBST and detected with anti-Mouse IgG-Horseradish peroxidase (GE Healthcare Life Science, Cat# NA931, 1:5000). For streptavidin staining ...
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bioRxiv - Biochemistry 2023Quote: ... to <1 mL and injected onto a Superdex™ 75 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20mM Tris pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with Cy5-probe ([23]) ...
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bioRxiv - Biochemistry 2023Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted protein was concentrated and incubated with the antibody fragment scFv16 for 2 h on ice at a molar ratio of 1:1.5.48 A Superdex 200 Increase 10/300 column (GE Healthcare) was pre-equilibrated with buffer [20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel was soaked in 1×TBE for 20 min and transferred to a Hybond-N+ membrane (GE Healthcare) for 1 h (15 V ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
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bioRxiv - Biophysics 2023Quote: ... the supernatant was filtered (0.22 μm pore size) and applied to a HisTrap HP 1 mL column (GE Healthcare) for affinity chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... Various Prp40 domains fused with an N-terminal Flag tag were cloned into pGEX-6p-1 vector (GE Healthcare) and purified from E.coli as GST fusion proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... PTENα-NTE (residues 1-173) was further purified by ion exchange chromatography (HiTrap SP HP column, GE Healthcare, 17115201) using buffers with 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... TbSmee1(1-400) concentrates were applied to a previously equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare) pre-equilibrated in dialysis buffer ...
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bioRxiv - Genomics 2024Quote: Ten μl of PCR product were then mixed with 1 μl of Streptavidin sepharose™ high performance (GE Healthcare) and 40 μl of PyroMark binding buffer (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 ml protein was loaded onto the analytical size exclusion column Superdex 200 increase 10/300 GL (GE Healthcare) in running buffer (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant for each nanobody was then applied to one 1 ml Ni-NTA pre-packed column (GE healthcare) pre-equilibrated with base buffer + 30 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... using 10/12% acrylamide/bisacrylamide (29:1) 0.1% SDS gels and transferred to a 0.2 μm PVDF Hybond-P membranes (Amersham/GE healthcare) at 20V for 1h in a semi-dry Trans-Blot SD cell system (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... was used in combination with a secondary anti-mouse antibody conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). Primary rabbit anti-mCherry antibody (BioVision 5993 1:2000 ...
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bioRxiv - Plant Biology 2020Quote: ... 1 mM EDTA) affinity chromatography followed by size-exclusion chromatography on a HiLoad 16/600 Superdex 200pg column (GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Biophysics 2021Quote: ... Cell lysates were centrifuged at 75,000 x g for 25 min and supernatants were loaded on a GSTrap FF 1 mL column (GE Healthcare) using an AKTA Start purification system (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was then supplemented with 5 mM imidazole prior to loading onto a 1-ml HisTrap column (GE Healthcare) attached to an AKTA system at 1 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... GST-PTP1B was purified with a 1 ml GSTrap FF Column on an ÄKTA pure purification system (GE Healthcare GmbH.) using a binary buffer system (buffer A ...
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bioRxiv - Cell Biology 2019Quote: ... Buffer was then exchanged to 1xSAB supplemented with 1 mM ATP to remove imidazole using PD-25 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Cell Signalling) and horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (respectively NA931V and NA934V. 1:10.000. GE Healthcare). Mineral dissolution activity of Oc was measured as described (Maurin et al. ...
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bioRxiv - Cell Biology 2020Quote: ... 2004) primary antibody and horseradish peroxidase linked anti-rabbit IgG secondary antibody (1:5000, from donkey, GE Healthcare Life Sciences). Signal was detected by chemiluminescence (Luminata Forte Western HRP substrate ...
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bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
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bioRxiv - Immunology 2021Quote: ... The diluted eluate was then loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, Chalfont St Giles, UK). After loading ...
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bioRxiv - Genomics 2020Quote: ... The membrane was incubated with 1:10,000 dilution of anti-rabbit IgG conjugated to horse radish peroxidase (HRP; GE Healthcare) diluted in 2.5% skimmed milk in TBS-T or anti-mouse IgG conjugated to HRP (GE Healthcare ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 3.0 (pH neutralization to 7.8 with 1 M Tris-HCl, pH 9.0) using ÄKTA FPLC system (GE Healthcare Life Sciences). The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime, GE healthcare). Chemiluminescence signals were detected by a LAS 4000 (GE healthcare ...
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bioRxiv - Genomics 2020Quote: ... Mouse Anti-Rabbit IgG (Light Chain Specific) (CST, #93702S) (1:5000) or Rabbit IgG HRP Linked Whole (GE Healthcare, NA9340), respectively ...