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Citations for GE Life Sciences :
301 - 350 of 5563 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: Gel imaging—Gels were imaged using an ImageQuant LAS 4000 imager (GE Healthcare). The band density of full-length H3 was used as a measure of intensity and was quantified using the ImageJ program (NIH) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated in standard procedures using 12% polyacrylamide gel (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (GE Healthcare Life science, Germany) using TransBlot semi dry transfer cell (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... Equivalent amounts of protein or equal volume from each sample were run on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (GE Healthcare, Piscataway, NJ, USA). Following blocking in 5% milk or bovine serum albumin the blots were incubated with primary antibodies and analyzed by HRP-conjugated secondary antibodies (Jackson Laboratories) ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were further resolved using size exclusion chromatography on gel filtration columns using the Superdex 200 (16/600) (GE Healthcare, GE28-9893-35) or Superdex 75 (16/600 ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were electrophoresed on an SDS polyacrylamide gel and then transferred to a nitrocellulose membrane (Amersham Protran Premium; GE Healthcare, Waukesha, WI, USA) using a semidry method ...
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bioRxiv - Neuroscience 2024Quote: ... We separated equal amounts of protein on 5-20% SDS‒PAGE gels (Wako, Osaka, Japan) and transferred them to Hybond-P membranes (GE Healthcare, Piscataway, NJ, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved by electrophoresis in a 10% or 12% gel and transferred to nitrocellulose membranes (GE Healthcare, Westborough, MA, #88018). The membranes were then incubated with specific antibodies ...
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bioRxiv - Microbiology 2021Quote: ... gel-purified using illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). 30 ng of each upstream and downstream flanking region of the targeted gene were used as templates for overlap ...
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bioRxiv - Molecular Biology 2020Quote: ... Gels were stained with Coomassie Blue (Phast Gel ™ Blue R, GE Healthcare, Sweden).
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bioRxiv - Biochemistry 2023Quote: ... and gel filtered using a Superdex 200 16/60 gel filtration column (GE Healthcare). The peak fractions were concentrated ...
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bioRxiv - Biochemistry 2023Quote: ... and gel filtered using a Superdex 200 16/60 gel filtration column (GE Healthcare). The peak fractions were concentrated ...
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bioRxiv - Biophysics 2021Quote: ... gel filtration columns (GE Healthcare). According to the separation efficiency ...
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bioRxiv - Cell Biology 2024Quote: ... PCR samples were resolved on agarose gel and amplified fragments extracted from gel using GFX PCR DNA and Gel Band purification kit (GE Healthcare, 28-9034-70). The purified DNA or 1µg of plasmid DNA were digested with XmaI and XbaI for 1 h at 37°C in 1x CutSmart buffer and nuclease-free water ...
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bioRxiv - Cancer Biology 2022Quote: ... All proteins were separated using SDS-polyacrylamide gel electrophoresis and transferred to PVDF Blotting Membrane (P 0.45, A29532146, GE healthcare Life science, Chicago, IL, USA) using the Trans-Blot Turbo Cassette (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: ... Cells were harvested and lysed in PBS supplemented with 1 mM PMSF and soluble protein was purified using nickel-affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare, Piscataway, New Jersey, USA). After SDS-PAGE analysis ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Gels were scanned on a fluorescence gel scanner (Typhoon FLA 9500, GE Healthcare, Freiburg, Germany) at 100 µm resolution ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... and gel-filtration (Superdex200, GE Healthcare) chromatography ...
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bioRxiv - Cancer Biology 2022Quote: ... The gel images were acquired on ImageQuant LAS4000 gel documentation system (GE Healthcare now Cytiva Life Sciences).
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bioRxiv - Cell Biology 2024Quote: Cell or collagen lysates were separated by denaturing SDS-PAGE (6% gels for collagens, 10% for SMADs) and proteins blotted onto a nitrocellulose membrane (Amersham™, GE Healthcare, Chalfont St Giles, United Kingdom). Membranes were cut horizontally to detect proteins of different sizes ...
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bioRxiv - Cell Biology 2022Quote: ... The polyacrylamide gels were then either imaged directly using a Typhoon 9200 Variable Mode gel scanner (GE Healthcare) (Cy5 filter setting ...
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bioRxiv - Microbiology 2020Quote: ... and subjected to gel filtration chromatography using a Hiload 16/60 Superdex 75 gel filtration column (GE Healthcare) running on an AKTA FPLC system ...
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bioRxiv - Microbiology 2022Quote: ... purified after agarose gel electrophoresis using the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare) and ligated into the linearized pHT315-gfp vector ...
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bioRxiv - Biophysics 2022Quote: ... with salt gradient of (250-1000mM NaCl for PARP1 and 50mM-1000mM NaCl for all other constructs) and finally through gel filtration (16/600 200pg or 16/600 75pg, GE Healthcare, depending on the size of the protein) column pre-equilibrated with buffer 150mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... followed by gel filtration chromatography (GE Healthcare). The S trimer of SARS-CoV-1 ...
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bioRxiv - Immunology 2022Quote: ... followed by gel filtration chromatography (GE Healthcare), as previously reported 66 ...
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bioRxiv - Immunology 2021Quote: ... and an ImageQuant Gel imager (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... and Superdex75 gel filtration chromatography (GE Healthcare) into 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: Protein extracts were precleaned with Protein G Sepharose (GE Healthcare) and subjected to immunoprecipitation with a combination of anti-FLAG M2 and Protein G Sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... protein G-Sepharose or protein A-Sepharose beads (GE Healthcare) for 2 h at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... separated by gel electrophoresis in 0.7% (w/v) agarose gels and blotted onto Hybond N nylon membranes (GE Healthcare). A ~500 bp probe was produced by PCR amplification (primer pair P19/P20 ...
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bioRxiv - Plant Biology 2020Quote: ... gels were firmly attached to one of the two glass gel plates via binding silane (GE Healthcare, Uppsala, Sweden) whereas the opposite plate was coated with a releasing agent (Blueslick ...
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bioRxiv - Microbiology 2020Quote: ... All standard proteins used were from GE’s Gel Filtration Low Molecular Weight Kit and GE’S Gel Filtration High Molecular Weight Kit (GE Healthcare), except for the BSA (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... and purified after agarose gel electrophoresis using the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare). Insert fragments were amplified with Q5 DNA polymerase (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... Protein (GE Healthcare), according to the manufacturer’s specifications in protocol “E,” except that glutardialdehyde (25% w/v ...
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bioRxiv - Biochemistry 2021Quote: ... 30 μg of total protein lysate was resolved on SDS polyacrylamide gel according to standard procedure at 20 mA per gel and blotted onto a nitrocellulose membrane 0.45 μm (GE Healthcare) via Mini Trans-blot module (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... PCR amplicons from each species presenting positive signals for Micropia were separated by 1.5% agarose gel electrophoresis and purified using Illustra GFXTM PCR DNA and Gel Band Purification kit (GE Healthcare) according to the supplier’s specifications ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were visualized on agarose gel then purified using PCR DNA and Gel Band Purification kit (GE Healthcare, UK).
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bioRxiv - Microbiology 2023Quote: ... Samples were purified using nickel-affinity chromatography followed by gel filtration using a Superdex-200 gel-filtration column (GE Healthcare), equilibrated in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... were resolved by SDS–polyacrylamide gel electrophoresis using a 10% acrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (GE Healthcare). After a blocking step (5% non-fat milk in TBS O/N at 4°C) ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated proteins were pulled down with protein A-sepharose (GE Healthcare) for immunoblotting ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A and Protein G beads mix (1:1) (GE Healthcare) were washed with HEMG100 ...
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
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bioRxiv - Cell Biology 2021Quote: ... and proteins were collected on protein G-Sepharose beads (GE Healthcare) for an additional 4 h at 4°C with gentle shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... a mixture of protein A and protein G sepharose (GE Healthcare life sciences ...
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bioRxiv - Biochemistry 2019Quote: ... followed by gel filtration (Superdex 75, GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... chromatography followed by gel filtration (Superdex200, GE healthcare). The chromatogram and SDS-PAGE analyses of the gel filtration step show that peak fractions contain a homogeneous 1:1 complex of Rad4 and Rad23 proteins ...
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bioRxiv - Microbiology 2019Quote: ... Gels were treated with Amersham Amplify (GE Healthcare) according to manufactures instructions ...