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Citations for GE Life Sciences :
201 - 250 of 5563 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated on 10% Tris-Tricine gels and imaged using a Typhoon 9410 Molecular Imager (GE Healthcare Life Sciences).
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: The recombinant proteins purified as described above were subjected to gel filtration assay (Superdex 200 Increase10/300 GL; GE Healthcare) in buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Immunology 2021Quote: ... the protein bands on SDS-PAGE gels were scanned and quantified by laser densitometry (Personal Densitometer SI; GE Healthcare, IL). The densities of the Hoc ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Physiology 2020Quote: ... YFP-tagged proteins were visualized by scanning the wet PAGE gels with a fluorescence scanner (Typhoon, GE Healthcare, München, Germany). Individual bands were quantified with ImageJ (53).
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bioRxiv - Biochemistry 2020Quote: ... The proteins were concentrated ,applied to gel-filtration using a Superdex 75 prep grade HiLoad 16/60 column (GE Healthcare) equilibrated in buffer K (10 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... the harvested protein was concentrated to approximately 2.0 ml and filtered using a Superdex 200 gel filtration column (GE Healthcare) equilibrated with buffer (20 mM Tris-HCl and 200 mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... Protein lysates were separated in 12% acrylamide gels and transferred to nitrocellulose blotting membrane (cat.# 10600004-GE Healthcare Life Sciences). The membrane was blocked with 5% skim milk in TBS-T (20 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... equal amounts of protein (30 µg) were run on homemade 10% polyacrylamide gels and transferred on nitrocellulose membranes (GE Healthcare). Membranes were probed with primary antibodies (listed in the table below) ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved on 6% SDS-PAGE gel and transferred to a nitrocellulose membrane (0.45 μm pore size, GE Healthcare) by Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Neuroscience 2022Quote: ... The protein was separated from free dye using gel filtration on a Superdex 75 pg 10/300 column (GE Healthcare).
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bioRxiv - Genomics 2022Quote: ... Separation was done on 8% bis-acrylamide gels in Tris-Glycine-SDS buffer and proteins were transferred on Amersham Protran 0.2 µm NC membranes (GE Healthcare) for western blotting ...
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bioRxiv - Biophysics 2022Quote: ... The biotinylated protein was separated from excess biotin on a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in 1x PBS buffer (10 mM Na2HPO4·7H2O ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each protein was then purified on a HiPrep™ 16/60 Sephacryl S-500 HR gel filtration column (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by electrophoresis on an 8% polyacrylamide gel and then transferred onto a nitrocellulose membrane (GE Healthcare) for 90 minutes at 100V ...
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bioRxiv - Plant Biology 2023Quote: Protein samples were resolved in a 12% SDS-polyacrylamide gel electrophoresis (PAGE) and then transferred to nitrocellulose membranes (GE Healthcare), blocked with 5% skimmed milk in TBST (Tris-buffered saline ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: Protein molecular mass at the native state was determined by gel filtration using a Superdex200 10/300 column (GE Healthcare). A gel filtration calibration kit (high molecular weight ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the protein of interest were applied to a Sephacryl 300 16/60 gel filtration column (GE Healthcare) and eluted with buffer D (10 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... The untagged protein was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare), eluted in buffer E (25 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... proteins from extracts were separated on SDS polyacrylamide gels and blotted onto Nitrocellulose membrane (Hybond ECL Cat#RPN2020D, GE Healthcare). Membranes were blocked using Skimmed Milk 5% ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing EftMHM4 or EftMPAO1 were pooled and the protein further purified by gel filtration chromatography on a Superdex200 10/300 column (GE Healthcare) equilibrated with gel filtration buffer containing 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The protein samples were centrifuged at 12,000g for 10 min to remove any protein precipitation and then loaded into a gel filtration column (Superdex 200 Increase 10/300GL, GE Healthcare) coupled with an 18 angle light scattering detector (DAWN HELEOS II ...
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bioRxiv - Biochemistry 2019Quote: ... of total cellular protein was separated by SDS-PAGE using 4-12.5 % Bis-Tris gels and transferred to nitrocellulose membrane (GE Healthcare # 10600006). SeeBlue pre-stained protein standard (Invitrogen # LC5925 ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the desired protein were pooled and gel filtration was performed on Superdex 75 or 200 10/300 GL columns (GE Healthcare) mounted into an ÄKTA P100 chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was further purified using reverse affinity chromatography on Ni-Sepharose followed by gel filtration (Superdex 200 16/60, GE Healthcare). Protein in gel filtration buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: Samples were electrophoresed on a 15% SDS polyacrylamide gel and proteins were then blotted onto a nitrocellulose membrane (GE Healthcare, Amersham). Nitrocellulose was blocked with 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Biochemistry 2020Quote: ... The target protein was eluted with 10 mM maltose and further purified via gel filtration on a Superdex S75 (GE Healthcare) column.
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bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing the target protein was further purified by gel filtration using a Superdex 200 10/60 GL column (GE Healthcare) equilibrated with the storage buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The cleaved protein was concentrated to 5 ml and loaded in a HiLoad 16/600 Superdex 75pg gel-filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... A second purification step was performed by loading Protein A eluate on a Hiload 16/600 Superdex 200 pg gel filtration column (GE Healthcare), which was equilibrated and run using DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated on 10 % acrylamide gels by SDS-PAGE and protein transferred to a 0.45 μm PVDF membrane (GE Healthcare 15259894) using the BioRad TransBlot Turbo system ...
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bioRxiv - Microbiology 2021Quote: The native molecular mass of the recombinant proteins was determined by gel filtration using a Superdex 200 10/300 column (GE Healthcare), previously calibrated with protein standards (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... The protein was further purified by gel filtration chromatography on a HiLoad™ 16/600 Superdex 200 pg column (GE Healthcare) with the following buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... protein extracts were resolved on SDS-PAGE gels under denaturing conditions and transferred to Hybond ECL membranes (Amersham Biosciences, GE Healthcare). Membranes were blocked for 1 hour at RT and incubated overnight with the respective primary antibody (Supplementary Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were further purified by gel filtration chromatography using a Superdex 200 column attached to an AKTA-format FPLC (GE Healthcare) and running buffer containing 50mM Tris pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 12 % denaturing acrylamide gel and subsequently transferred onto a nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membrane was blocked using 1x Roti®Block (Carl Roth ...
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bioRxiv - Biophysics 2022Quote: ... The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... The eluted protein fraction was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) in the buffer of 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted proteins were analyzed in an 8% SDS-polyacrylamide gel and scanned and quantitated using a Typhoon 9400 laser imager (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The protein in the supernatant was sequentially purified using a Ni-NTA column and a Superdex200 gel filtration column (GE Healthcare) as previously described (20) ...
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bioRxiv - Biophysics 2020Quote: ... The protein in the supernatant was collected and subjected to gel filtration chromatography in a Superose6 10/300 GL column (GE Healthcare) previously equilibrated in Buffer B1 with 0.02% DDM ...
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bioRxiv - Zoology 2020Quote: ... Proteins were separated in the second dimension on a 14% SDS polyacrylamide gel (Laemmli, 1970) in a Hoefer600 cell (GE Healthcare) for 15 min at 15 mA (100 V/15 W limits ...