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Citations for GE Life Sciences :
301 - 350 of 379 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... gel analysis were pooled and dialyzed against Tris-HCl buffer (30 mM Tris-HCl, pH 8) using PD-10 Desalting Columns (GE Healthcare). The protein concentration was determined by Bradford assays with BSA (bovine serum albumin ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... Proteins were further purified by concentrating to ∼3mg/ml and applying to a size exclusion column (Superdex-200 increase, 10/30 GE Healthcare) equilibrated in buffer A containing 5 mM ATP ...
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bioRxiv - Microbiology 2023Quote: ... The crude extract was centrifuged at 35,000 g for 30 minutes and then passed over Ni Sepharose HP (GE Healthcare, Solingen). The target proteins were eluted with an imidazole gradient ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: Proof-of-concept data were acquired in six healthy volunteers (five female, mean age 30 years, range 24 – 46 years) on a simultaneous 3T SIGNA PET-MR scanner (GE Healthcare); ethics approval was granted by the University of Manchester Research Ethics Committee (reference ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 1mM DTT) and using a gradient of 30–1000 mM NaCl followed by size-exclusion chromatography (Superdex 75 16/60, GE Healthcare) in buffer B [25 mM Tris-HCl pH 7.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Images were acquired with 200 ms exposure time every 6 minutes for at least 6 h at 30°C using Softworx (Applied Precision) software ...
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bioRxiv - Cell Biology 2022Quote: Cellular lysates were separated on a Superose 6 HR 10/30 size-exclusion column and an AKTA Purifier protein purification system (GE Healthcare), essentially as described previously(61) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 M NaCl and 30 mM imidazole and captured with Glutathione-Sepharose 4B beads as indicated by the manufacturer (GE Healthcare). The elution fractions were pooled and the buffer was exchanged to phosphate-buffered saline (PBS ...
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bioRxiv - Genetics 2023Quote: ... The microfluidic Y04E plate was then loaded into an environmental chamber heated to 30°C mounted on a DeltaVision Elite wide-field fluorescence microscope (GE Healthcare) with a PCO Edge sCMOS camera and operated by the associated softWoRx software ...
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bioRxiv - Biophysics 2023Quote: ... The fixed cells were rinsed three times in warm PBS and incubated for 30 min with a blocking solution containing 1% BSA (GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... a final gel filtration step was performed in GF buffer (30 mM HEPES pH 8, 250 mM NaCl, 10 mM TCEP) using a S200 size exclusion chromatography column (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... The peak associated with pure ACBP was concentrated and further purified by SEC using a Superdex 75 (HR 10/30) column coupled to an Äkta purifier system (GE Healthcare) using Buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was concentrated to 100 µL using a 100 kDa MWCO concentrator and the concentrate was loaded onto a size-exclusion chromatography (SEC) column (Superose 6 Increase 10/30 GL, GE Healthcare), equilibrated in a buffer composed of 20 mM Tris-Cl (pH 8) ...
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bioRxiv - Plant Biology 2024Quote: ... The enriched nuclei were further purified using three-step Percoll-gradients (100%/60%/30%, 17-0891-01, GE Healthcare, Solingen, Germany) modified after Marienfeld et al ...
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bioRxiv - Microbiology 2024Quote: ... Plates were incubated at 30 °C for 48 h and pictures were taken with Amersham Imager 600 (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2024Quote: ... The tissue was then filtered on a cell strainer (70 µm) and the cells were separated from myelin and debris with a 30% Percoll gradient (17-0891-01, GE Healthcare) in HBSS without Ca2+ and Mg2+ ...
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bioRxiv - Neuroscience 2024Quote: ... cell lysates were diluted with supplemented TEEN buffer to a final concentration of 2 µg/µL (approximately 1.6 mg of protein) and pre-cleared by incubation with 30 µL of protein-A Sepharose (GE Healthcare, #11514945) for 1 hour at 4°C under rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... was concentrated to 0.5 mL on a Vivaspin 20 MWCO 30 filter and was injected into a Superose 6 Increase 10/300 GL column (GE Healthcare) in buffer H200 (20 mM Hepes ...
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Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexesbioRxiv - Biophysics 2024Quote: ... The solution was then adjusted to 2 M KCl in TE 10/50 buffer for size exclusion chromatography (SEC) using a XK 50/30 column packed with Sepharose 6 Fast Flow (GE Healthcare). Eluted fractions were analyzed on a 1% agarose gel ...
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bioRxiv - Biochemistry 2024Quote: ... incubated at 4 °C for 30 min and purified using gel filtration (Superdex 200 Increase 10/300 GL) columns (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... The clarified lysate obtained after centrifugation (30 min at 20000 × g, 4°C) was first run on a streptavidin affinity column (StrepTrap® HP, GE Healthcare) equilibrated with septin 1 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved topo VI fractions were pooled and concentrated using Amicon Ultra 15 mL centrifugal filter units (30 kDa cut off, MilliporeSigma) before being passed down a Superose 6 10/300 (GE Life Sciences) column in Buffer C (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... was transferred from the purification buffer into the labeling buffer (Na-PO4 20 mM, pH 8.3) supplemented with OG (30 mM) using PD-10 columns (GE Healthcare, Solingen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then centrifuged (21 K, 4°C, 30 min) and the supernatant applied to an AKTA Start (GE Life Sciences) at 5 mL min−1 ...
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bioRxiv - Microbiology 2021Quote: ... Then the lysis was centrifuged at 15000 g for 30 min and the supernatant was loaded onto HisTrap HP column (GE Healthcare, America). The column was then washed with wash buffer (lysis buffer supplemented with 30 mM imidazole ...
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bioRxiv - Genetics 2020Quote: ... the eluted protein solution was concentrated 10 times in a Vivaspin concentrator 30 kDa cut off and loaded in a Superose 6 increase 10/300 (GE Life Sciences) with PBS as a carrier buffer ...
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bioRxiv - Biochemistry 2021Quote: Protein complexes were separated according to relative molecular mass using an Äkta™ FPLC system with a Superdex 200 HR 10/30 column (GE Healthcare) and 24 × 106 cardiomyocytes per sample ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA from 30–50 light-treated or dark control embryos was extracted using the illustra™ RNAspin Mini kit (GE Healthcare). 1 μg total RNA was used for reverse transcription with qScript XLT cDNA SuperMix (Quantabio) ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown in appropriate synthetic dropout media at 30°C to mid-log phase (OD600 of ∼0.5) and imaged using DeltaVision Elite system (GE Healthcare Life Sciences) equipped with an Olympus IX-71 inverted microscope ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged in a Beckman 45Ti rotor at 100,000 x g for 30 min at 4 °C and the supernatant applied to a HiLoad 26/10 Q Sepharose column (GE Life Sciences) equilibrated in 90 % buffer A and 10% buffer B (buffer A ...
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bioRxiv - Microbiology 2021Quote: ... Peak fractions were concentrated with 30 kDa cutoff concentrators (Amicon) to ∼10 mg/ml and loaded onto a S200 Increase 10/300 GL (GE Life Sciences) in SEC Buffer [20mM Tris ...
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bioRxiv - Immunology 2021Quote: ... denatured in Denaturation buffer [0.5N NaOH and 1.5M NaCl (Fisher)] for 30 min with gentle agitation and then transferred to Hybond-XL membranes (GE Healthcare Amersham/Fisher). Synthesized DNA specific to regions of homologous recombination (see Oligonuleotide section in Key Resources Table ...
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bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... Images were captured one frame every 1 min 30 sec over 5 h using a DeltaVision Imaging System (Applied Precision, Bratislava, Slovakia).
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bioRxiv - Neuroscience 2023Quote: ... then centrifuged (400g for 10 minutes) and the resulting pellet was re-suspended in 30% Percoll (GE Healthcare BioScience AB, Uppsala, Sweden) and centrifuged at 500g for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: ... then concentrated in a 10 kDa NMWL centrifugal filters (Amicon #UFC9003) to ∼500 μL and injected on a Superdex 200 10/30 GL size exclusion column (GE Healthcare #28990944) equilibrated in size exclusion buffer (SEB ...
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bioRxiv - Biochemistry 2023Quote: ... The synthesized DNA products were separated on denaturing 30% PAGE with 7 M urea in 1xTBE and visualized on a Typhoon 9400 (GE Healthcare, USA).
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bioRxiv - Cell Biology 2023Quote: ... using a series of chromatography steps (XK 50/30 cationic exchange column [GE Healthcare] packed with SP-Sepharose Fast Flow [GE Healthcare] ...
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bioRxiv - Genomics 2024Quote: ... The supernatant from the second and third digests were combined and loaded onto a 30%/35%/45%/50% Percoll discontinuous gradient consisting of 90% Percoll (GE Healthcare, #17089101) mixed with 25 mM HEPES in HBSS in the appropriate concentrations ...
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bioRxiv - Microbiology 2024Quote: ... The crude cell extract was cleared by centrifugation for 30 min at 16,000 ×rpm (4 °C) and loaded onto a 5 ml HisTrap™ HP affinity column (GE Healthcare, USA) equilibrated with buffer B ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractions containing protein were concentrated to 1 mL using an Amicon 100,000 MWCO (or 30,000 MWCO for HrpA1–783 and HrpA1–1179) device and further purified by size exclusion chromatography on a 10/30 Superdex™ 200 column (GE Healthcare) in 10 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2024Quote: ... 4°C for 30 min and added to a 50-mL Falcon tube holding 6mL of Glutathione-Sepharose® 4B resin (GE Healthcare) that was pre-equilibrated in the same buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... to separate blood constituents and isolation of buffy coat density gradient centrifugation (400 × g at 18°C for 30 min) of RPMI-1640 diluted blood over a Ficoll-Paque(tm) Plus reagent (Cat. 17-1440-02, GE Healthcare, Piscataway, NJ) was performed ...
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bioRxiv - Biochemistry 2021Quote: ... or by sonication and cleared lysate was obtained by centrifugation (24,446 x g; 30 min; 4°C) and filtration using a Whatman™ folded filter (GE Healthcare, now Cytiva).
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bioRxiv - Biophysics 2020Quote: ... the Mono Q elutions containing desired proteins were further purified by concentration on Amicon Ultra filters followed by size-exclusion chromatograph using a Superdex 200 10/30 GL column (GE Healthcare Life Sciences) in GF150 buffer (25 mM HEPES pH 7.5 ...
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Cytokine and phenotypic cell profiles in human cutaneous leishmaniasis caused by Leishmania donovanibioRxiv - Microbiology 2022Quote: Peripheral Blood Mononuclear Cells (PBMC) were isolated from EDTA blood by density gradient centrifugation using 30% of Ficoll-Paque (GE Life Sciences, country). Cells were incubated at 37°C in 5% CO2 and monocytes were separated by adherence after 24 hours and cultured in complete RPMI1640(Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant obtained after centrifugation at 15,000 rpm for 30 min was purified using a HisTrap™ HP column (GE Healthcare, Chicago, Illinois). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were subsequently washed with 1 x TBS/T for 30 min in 10 min intervals prior to incubation in ECLTM (GE Healthcare, cat# RPN2106) and exposure by film (FUJIFILM Super RX ...