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Citations for GE Life Sciences :
201 - 250 of 379 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The cleared lysates containing the protein of interest were incubated 4h at 4°C with 1.5 μM of the indicated GST-fused proteins and 30 μl of glutathione-sepharose CL-4B beads (GE Healthcare). After three washes in lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.1% FA (Fluka) and crosslinked peptides were enriched by separation on a Superdex Peptide PC 3.2/30 column (GE Healthcare), using an Agilent 1100 chromatography system at 95 μL/minute with fraction collection ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation for 30 min at 47,000 xg and the supernatant was loaded onto a 5 ml GSTrap column (GE Healthcare). The resin was washed by the lysis buffer and bound protein was eluted with elution buffer of 100 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble material was sedimented by centrifugation at 13,500 g for 30 min at 4°C prior to loading on His-trap FF columns (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and the recombinant protein was purified from the soluble fraction (separation after 30 min 10,000 rpm) using a Ni2+-NTA-agarose column (GE Healthcare), equilibrated in 50 mM Tris-HCl ...
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bioRxiv - Pathology 2021Quote: ... the crude protein solution was finally collected by ultracentrifugation (40,000 rpm, 30 min, 4°C) and subsequently incubated with Ni-NTA (GE healthcare) resin for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed and stained with 0.4% methylene blue in methanol for 30 min and images acquired with an image analyzer (Amersham Imager 600, GE healthcare).
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bioRxiv - Neuroscience 2020Quote: Protein samples concentrated to ∼1.0 g/L were injected into an HPLC-driven SEC column (Superdex 200 10/30 column, GE Healthcare) equilibrated with HBS-C buffer ...
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OxoScan-MS: Oxonium ion scanning mass spectrometry facilitates plasma glycoproteomics in large scalebioRxiv - Systems Biology 2022Quote: ... IgG was isolated from 5 μl of serum using 30 μl of Protein A Sepharose (GE Healthcare, Eindhoven, The Netherlands). Sample mixtures were incubated under agitation at 650 rpm for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µL of sample (10 µl injection loop loaded with 50 µL sample) were injected on a Superdex 75 PC 3.2/30 (GE Healthcare) analytical size exclusion column preequilibrated with the protein buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were cleared by centrifugation at 100,000 g at 4°C for 30 min and then mixed with Glutathione Sepharose 4B (GE Healthcare). The Sepharose beads were washed with buffer B [50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2020Quote: ... Labeled NBD1 was spun at 4 °C and 16,000 g for 30 minutes and passed through a gel filtration column (Superdex 200 Increase 10/300 GL - GE Healthcare) previously equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The crosslinker was inactivated with 30 mM Tris pH 8.0 and the sample was loaded onto a Superose 6 Increase 10/300 GL (GE healthcare) column equilibrated in GF buffer (30 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were removed by centrifugation at 40,000 rpm for 30 min and supernatants were loaded onto Superose 6 Increase (GE Healthcare) for FSEC analysis.
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bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μl of the concentrated 26S proteasome sample (alone or mixed with 90 μM of model degron) was further purified by size exclusion chromatography using a Superose 6 PC 3.2/30 column (GE Healthcare), equilibrated with 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... and lysates were clarified by centrifuging at 30,000g for 30 minutes and the supernatant was incubated with Ni resin (Ni Sepharose Fast Flow, GE Healthcare) for 30 minutes at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The medium from 1 L of culture was filtered using a 0.22-μm membrane and the filtrate was adjusted to contain 30 mM imidazole before incubating with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) beads for 2 h at 4 °C with mild agitation ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were spun down at 12,000 g for 30 min and the supernatants were loaded onto a His-Select Nickel Affinity gel (GE Healthcare). Recombinant PstS proteins were eluted with the elution buffer (500 mM imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant and the crude lysate were separately filtered using a 0.2 μm syringe Filter (Puradisc FP 30 Cellulose Acetate Syringe Filter, GE Healthcare) and directly loaded onto the column at 1 mL/min one after another ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were loaded into a 30 μL loop and applied to a Superdex 75 3.2/300 increase column (GE healthcare) equilibrated in 50 mM Tris pH 7.5 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A second purification step was performed by size-exclusion chromatography (SEC) with Superdex 75 column (1 x 30) (GE Healthcare) at a flow rate of 0.5 mL/min monitored at 280 nm using a 25 mM Na2HPO4/NaH2PO4 ...
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bioRxiv - Neuroscience 2021Quote: ... equal amounts of protein (30 µg) were run on homemade 10% polyacrylamide gels and transferred on nitrocellulose membranes (GE Healthcare). Membranes were probed with primary antibodies (listed in the table below) ...
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bioRxiv - Microbiology 2022Quote: Neutrophils were purified by centrifugation for 30 min at 1,300xg on a discontinuous Percoll gradient (GE Healthcare #17-0891-01) consisting of 62% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... The cells were grown and maintained in DMEM/F12 medium (ATCC 30-2006) supplemented with 10% heat-inactivated fetal bovine serum (FBS - SH30071.03, HyClone-GE healthcare), 0.5 mg/ml G-418-sulfate (10131-027 ...
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bioRxiv - Biophysics 2023Quote: ... the sample was clarified 400,000 x g for 30 minutes and applied to a Superdex 200 Increase 10/300 GL (GE Healthcare) gel filtration column equilibrated in G-buffer containing 0.2 M ammonium acetate ...
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bioRxiv - Biochemistry 2023Quote: ... and the concentrate was loaded onto a size-exclusion chromatography (SEC) column (Superose 6 Increase 10/30 GL, GE Healthcare) connected to a Shimadzu high-pressure liquid chromatography instrument ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 30,000 g for 30 min at 4°C and incubated for 45 min with amylose beads (GE Healthcare). The loaded protein was cleaved on column by GST-tagged 3C protease at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... The soluble fraction was separated by centrifugation at 30,000×g for 30 min and subjected to size exclusion chromatography using a HiLoad26/60 Superdex75 column (GE Healthcare) with gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The washing step was followed by 30 min incubation with HRP-conjugated Mouse Anti-M13 monoclonal antibody (GE Healthcare, 27942101) diluted in PBST in 1:5000 ratio ...
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bioRxiv - Neuroscience 2024Quote: ... and 30 µL of each diluted odor was placed on a syringe filter (2.7-µm pore size, 6823-1327, GE Healthcare). Odorized air was further diluted with filtered air by 1:8 to produce a 1 liter/min total flow rate ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins (30 µg/lane) were resolved by SDS-PAGE and electro-transferred to the Hybond-P PVDF membranes (GE Healthcare). Membranes were probed at 4◦C overnight with mouse monoclonal anti-Myc-Tag antibodies (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The eluent was pooled and concentrated to a volume of ∼0.5 mL with a 30-kDa Vivaspin concentrator (GE Healthcare) and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was cleared by centrifugation at 30,000 g for 30 min and the supernatant was loaded onto a HisTrap FF crude column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... with a molecular weight cut-off (MWCO) of 30 kDa and polished by size exclusion chromatography on a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation (15’000 xg, 4 °C, 30 minutes) and supernatants were incubated with 1 ml Glutathione Sepharose (GE Healthcare) for 2 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysate was centrifuged at 4 °C at 11,000 rpm for 30 min and the supernatant was then loaded onto a Ni-affinity chromatography column (GE Healthcare), which was pre-equilibrated using the lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 4°C at 10,000 g for 30 minutes and the supernatants were used to purify target proteins by Ni NTA agarose (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... the lysate was centrifuged at 45,000 g for 30 min and His-(MBP-) ZNF524 further purified on a HisTrapTM HP (GE Healthcare) via the Akta Prime Plus System ...
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bioRxiv - Immunology 2023Quote: ... Blots were then wash 3 times in 15-30 mL TBST (0.05% v/v) and chemiluminescence were quantified on the Amersham Imager 680 (GE Healthcare). All immunoblots shown are representative of 3 or more biological replicates.
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bioRxiv - Cell Biology 2023Quote: ... The solution was then passed over a Superose 6 Increase 10/30 size-exclusion chromatography column (GE Healthcare Life Sciences) that was preequilibrated in 50 mM phosphate buffer pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... the column was equilibrated with solvent A (30 % ACN, 0.07 % TFA) and connected to an Aekta FPLC system (GE Healthcare). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation at 40,000 x g for 30 min and incubated with 10 mL Glutathione Sepharose 4B (GE Healthcare) for 14 h ...
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bioRxiv - Microbiology 2024Quote: ... and amplified by Multiple Displacement Amplification (MDA) at 30°C for 2h using the Illustra GenomiPhi v3 kit (GE Healthcare). The SSU rRNA gene from each isolate was then amplified by PCR using the MDA product as template ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were suspended in 30 µL of an aequous solution containing Tris (50 mM, GE Healthcare, Chicaco, IL, USA), NaCl (150 mM ...
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bioRxiv - Biochemistry 2024Quote: ... Elution was performed with a gradient of 0.225–1.5 M NaCl in buffer K using Äkta FPLC (gradient length 30 mL, flow rate 1 mL/min, GE Healthcare). CF eluted at ∼825 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... The desalted samples were then fractionated by peptide-level size-exclusion chromatography (SEC) using a Superdex 30 Increase column (300 x 3.2 mm; GE Healthcare) (mobile phase ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM HEPES pH 7.5 (Buffer B) supplemented with 30 mM imidazole before elution directly into a PD-10 column (GE Healthcare; equilibrated in Buffer B + 20 mM imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein extracts (30 µg) were separated by SDS-PAGE and transferred to Hybond-C Extra nitrocellulose membranes (GE Healthcare, USA). Details regarding primary and secondary antibodies are listed in Table S5 ...