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Citations for GE Life Sciences :
301 - 350 of 5743 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The eluate was subjected to size-exclusion chromatography on a 20 ml Superdex 200i 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 10 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Molecular Biology 2021Quote: ... was transferred from the purification buffer into the labeling buffer (Na-PO4 20 mM, pH 8.3) supplemented with OG (30 mM) using PD-10 columns (GE Healthcare, Solingen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... The amine modified dT nucleotides were labeled with Cy5 in a 20 µL reaction containing 10 mM (200 nmol) NHS-Cy5 (GE Healthcare, PA15100), 0.1 mM (2 nmol ...
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bioRxiv - Cancer Biology 2023Quote: ... to 20 mg/mL and purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). Fractions containing pure Ras protein were pooled and concentrated to 20 mg/mL and stored at –78 ºC ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was diluted 1:10 v/v with 20 mM Tris 8.0 and further purified with anion exchange chromatography (HiTrapQ column, GE Healthcare Life Sciences) using a NaCl gradient of 50 mM to 500 mM in 20 mM Tris 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Complexes were incubated for 20 minutes at 23 ℃ before further purification via SEC on a Superdex 200 Increase 10/300 GL (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
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bioRxiv - Physiology 2024Quote: ... was used to induce anaesthesia via a 22 gauge intravenous catheter which was placed in the auricular vein (Carestastion 650, GE Healthcare, UK). Anaesthesia was maintained using inhaled sevoflurane (SevoFlo ...
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bioRxiv - Cell Biology 2022Quote: ... and 15% (v/v) fetal bovine serum (GE Healthcare Life Sciences HyClone Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded onto a 6mL SOURCE 15S column (GE Healthcare), and eluted with a linear gradient of 0-50% high salt buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... 15% Defined fetal bovine serum (HycloneTM, GE Healthcare #SH30070.03), 1% MEM non-essential amino acid solution (Sigma #M-7145) ...
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bioRxiv - Immunology 2020Quote: ... layered onto 15 ml Ficoll-Paque Plus (GE Healthcare) and then centrifuged at 400 xg for 30 minutes at room temperature without brake ...
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bioRxiv - Plant Biology 2021Quote: ... GFP or mCherry fluorescence was measured 4-5 days post-infiltration (see Table S3 for raw data) using the ImageQuant LAS 4000 luminescent imager (GE Healthcare Life Sciences) with the appropriate excitation and emission settings (EX ...
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bioRxiv - Biophysics 2020Quote: ... DCS experiments with working buffer solutions containing 4 μM of an SBD-mutant and 5 mM substrate were conducted on a VP-DSC Calorimeter (Micro Cal™, GE Healthcare, Buckinghamshire). The melting temperature Tm was determined by ORIGIN 8 (Origin Lab Corp ...
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bioRxiv - Immunology 2021Quote: ... and was then applied to a 24 mL Superdex 75 increase (GE Healthcare) column equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... and purified by SEC using a 24 mL Superdex 75 column (GE Healthcare) to separate the H2A-Ub from Ub species.
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at 4°C overnight prior to removal of unreacted FITC by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... were cultured according to the ATCC guidelines in 4:1 mixture of Dulbecco’s medium and Medium 1999 supplemented with 10% Hyclone™ FBS (GE Healthcare) and 50μg/ml Gentimicin (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4°C by injecting sample onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Immunology 2020Quote: ... Samples were separated by protein electrophoresis at 120 V for 2 h using 10% Bis-Tris pre-cast gels (NuPage) and transferred overnight at 4°C onto Hybond-P PVDF membranes (GE Healthcare). After blocking in ECL blocking agent (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... mosaic NP preps were stored for a month at 4°C and then analyzed via SEC using a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Cell Biology 2020Quote: ... 100 – 300 µg protein was loaded on 10 – 12% SDS-PAGE and transferred at 4°C overnight to a nitrocellulose blotting membrane (A29434119, GE Healthcare) in transfer buffer (25 mM Tris pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... These solutions were prepared either by dialysis against the buffer at 4°C or by passage through PD-10 desalting columns (GE Healthcare). Protein concentrations were measured spectrophotometrically using known values for the extinction coefficient ...
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bioRxiv - Biophysics 2023Quote: ... 3,000 g × 15 min at 4 °C) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex-75 Increase 10/300 GL, GE Healthcare). The unfolding and refolding process for GlnBP WT was conducted under the same conditions as described for the GlnBP variants.
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bioRxiv - Biochemistry 2024Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4 °C by injecting sample onto a Superose 6 Increase 10/ 300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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bioRxiv - Biophysics 2022Quote: ... proteins were concentrated by ultrafiltration at 4 °C (MWCO: 10 kDa) and passed over a Superdex 75 26/60 gel filtration column (GE Healthcare) equilibrated in 20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
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bioRxiv - Plant Biology 2024Quote: ... The mixture was centrifuged at 2,000 g and 4 °C for 10 min and eventually filtered with 0.43 μm organic phase medium (GE Healthcare, 6789-0404). The column was a 100×2 mm Phenomenex Luna 3 μm NH2 (Catalog No. ...
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bioRxiv - Biochemistry 2024Quote: ... incubated at 4 °C for 30 min and purified using gel filtration (Superdex 200 Increase 10/300 GL) columns (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
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bioRxiv - Biochemistry 2022Quote: ... to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare). A linear 0-75 mM histidine gradient with elution buffer was used to elute proMbxA ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mL imidazol gradient) or by size exclusion chromatography (SEC) using a Superdex® 75 10/300 GL (GE Healthcare) with a flow rate of 0.5 mL/min and dialysis buffer as running buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 mM dithiothreitol (DTT), and concentrated using Vivaspin 5 kDa (HipBLp, HipSLp) and 10 kDa (HipTLp) cutoff columns (GE Healthcare). Protein concentration was quantified using the Bradford assay according to the manufacturer’s instructions (BioShop) ...
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bioRxiv - Biophysics 2020Quote: ... buffer twice and then subjected to size-exclusion chromatography on a 20 ml Superdex 200i 10/300 GL column (GE Healthcare Life Sciences) for detergent removal.
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bioRxiv - Biophysics 2022Quote: ... was titrated into an A1 protein solution (22 μM except 50 μM with A1+OGly+N+C) in a MicroCal iTC200 (GE Healthcare Life Sciences). A priming injection of 0.4 μL (not included in data analysis ...
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bioRxiv - Biophysics 2022Quote: The rat Syb2 (29–93) and Syb2 (29–93, S61C), mouse Rab3A (22–217, Q81L) were all cloned into pGEX-6p-1(GE Healthcare; Piscataway, NJ). The cytoplasmic domain of rat Syx1 (2–253 ...
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Intradermal Administration of Rescue Dose of Antiemetics in Chemotherapy-induced Nausea and VomitingbioRxiv - Pharmacology and Toxicology 2023Quote: ... and skin thickness was measured using an ultrasound diagnostic device (LOGIQ e Premium Pro, L10-22-RS probe, GE Healthcare Japan, Tokyo, Japan) and an ultrasound gel (LOGIQLEAN bottle hard type ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 20 mL (GE Healthcare) with loading buffer (10 mM Tris (pH 7.6) ...
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bioRxiv - Microbiology 2020Quote: ... Sephadex LH-20 (GE Healthcare) and Bio-Beads SM2 resin (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... filled with 15 ml of Ficoll-Plaque PLUS (GE Healthcare) through a 15 mins centrifugation at 1,200 g ...
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bioRxiv - Biochemistry 2022Quote: ... first on a Superdex 200 15/300 column (GE Healthcare), and then again on a Superdex 200 5/150 (GE Healthcare).
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bioRxiv - Genomics 2022Quote: ... supplemented with 15 % fetal bovine serum (GE Healthcare Life Sciences), 5 μM beta mercaptoethanol (Sigma) ...