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Citations for GE Life Sciences :
301 - 350 of 2796 citations for 8 HYDROXY 2 METHYL 4 1H PYRAZOL 1 YL QUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Proteins were electrophoretically separated on an 8% polyacrylamide gel and then wet- transferred to a nitrocellulose membrane (Amersham Protran 0.2 NC, GE Healthcare). Prior to antibody incubation ...
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bioRxiv - Bioengineering 2021Quote: ... The UtAs were scanned using the C4-8 transabdominal probe of a GE Voluson E10 (GE Healthcare, Wisconsin, United States) US machine ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15 M (NH4)2SO4 pH 8) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then dialysed into buffer C (1 M (NH4)2SO4 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 million cells were injected subcutaneously in the flanks of 8 week-old Nude or NSG mice with a ratio of 50/50 standard medium (DMEM: Cat# SH30022.01, GE Healthcare) and BD Matrigel™ (Cat# 356234 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA products were extracted with phenol:chloroform:isoamyl alcohol and analyzed on an 8% urea-PAGE using fluorescence scanner (Typhoon™ FLA7000, GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... elution in buffer A plus 350mM imidazole (pH 8) and gel filtration chromatography on a Superdex 200 column (GE Healthcare) in buffer A.
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bioRxiv - Biochemistry 2022Quote: ... Afterwards the lysates were subjected to SDS-polyacrylamide gel electrophoresis using an 8% polyacrylamide gel and then electroblotted onto a nitrocellulose membrane with a pore size of 0.45 μm (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... beads were washed once in lysis buffer and rotated at 4ºC overnight in wash buffer containing 8 U/ml thrombin (GE healthcare) to remove the GST tag ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by electrophoresis on an 8% polyacrylamide gel and then transferred onto a nitrocellulose membrane (GE Healthcare) for 90 minutes at 100V ...
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bioRxiv - Microbiology 2023Quote: ... elution in buffer A plus 350mM imidazole (pH 8) and gel filtration chromatography on a Superdex 200 column (GE Healthcare) in buffer A.
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bioRxiv - Neuroscience 2023Quote: The experiments were conducted using a 3-Tesla scanner equipped with an 8-ch phased array coil (GE Healthcare, Signa). Functional images were acquired using a T2*-weighted gradient-echo echo-planar imaging sequence ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were further enriched by centrifugation at room temperature at 695 g for 8 min in 37.5% Percoll (GE Healthcare), prior to washing and resuspension for downstream analysis.
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bioRxiv - Biochemistry 2022Quote: ... was passed through the HisTrap column previously equilibrated with IMAC buffer A at 2 ml/min flowrate with a peristaltic pump (GE Healthcare Pump P-1) and then washed with 5 CVs of buffer IMAC A supplemented with 50 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... were mixed together at a molar ratio of 1:2 and loaded on a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare, Chicago, IL, USA) pre-equilibrated with 20mM Tris-HCl (8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 18000-20000 rpm at 4 °C and supernatant was allowed to bind to Glutathione resin (GS resin) (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat no. 17-5132-02) in a batch binding mode for overnight at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-16-cell embryos were transferred to 3 % Ficoll (GE Healthcare) in 0.6x MMR and injected with 5-10 nl of a solution containing mRNAs and/or MOs ...
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bioRxiv - Biochemistry 2020Quote: ... SDS-PAGE gels (15-well NuPAGE 4–12% BisTris, GE Healthcare) revealed a single band for the purified enzymes ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose 4 Fast flow beads (GE Healthcare Life Sciences) washed twice with wash buffer were added to each IP and incubation was continued for 2 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... and Protein G Sepharose 4 Fast Flow Media (#11524935, GE Healthcare) to pull-down phosphorylated IRF5 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NHS-activated Sepharose 4 Fast Flow was obtained from GE Healthcare and protein ladder from Thermo Fisher Scientific.
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bioRxiv - Cancer Biology 2019Quote: ... 30 µL of protein G sepharose 4 fast flow (GE Healthcare) was bound to 5 µL of anti-HN1 (GeneTex ...
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bioRxiv - Biochemistry 2019Quote: ... GluC was removed with benzamidine Sepharose 4 Fast Flow (GE Healthcare) resin ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant was incubated with Gluthathione Sepharose 4 Fast Flow (GE Healthcare) for 2 hours at 4 °C ...
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bioRxiv - Genetics 2021Quote: ... Glutathione Fast Flow Sepharose 4 beads (GE Healthcare, 17-5132-01) that had been pre-washed in binding buffer were then added to the binding reaction and it was incubated in a rotor for 2 hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 3 or 4 of a Series S CM5 chip (GE Healthcare) functionalised with an anti-His capture kit (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Protein G Sepharose 4 Fast Flow (17-0618-01, GE Healthcare), Dynal magnetic beads (110.31 ...
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bioRxiv - Biophysics 2023Quote: ... and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare), and Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Bioengineering 2024Quote: ... at 4 °C using an ÄKTA Pure 25 M (GE Healthcare). The column was washed with 75 mM imidazole [in 20 mM NaH2PO4 and 0.5 M NaCl (pH 7.4)] ...
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bioRxiv - Biochemistry 2021Quote: ... and diluted with an equal volume of 50 mM Tris pH 8 before loading onto HiTrap Heparin HP columns (GE Healthcare). After extensive washing with 50 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Biochemistry 2020Quote: ... All products were resolved on 12% polyacrylamide gels containing 8 M urea and visualized using Typhoon Trio fluorescence imager (GE Healthcare).
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Biochemistry 2019Quote: SEC-MALS was conducted using a Wyatt Dawn 8+ detector (Wyatt Technology) coupled to a Superdex 75 10/300 column (GE Healthcare) and a refractive index detector ...
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bioRxiv - Neuroscience 2020Quote: ... The lysates were centrifuged at 18000 rpm (F21-8×50y rotor) for 45 min and supernatants were purified by Ni2+-affinity chromatography (FPLC system ÄKTA Purifier, GE Healthcare) eluting the protein in 50 mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were separated by denaturing (8 M urea) 10% PAGE and were visualized by using a Typhoon phosphorimager (GE Healthcare). As described previously,48 band intensities were determined and used to estimate the fraction of RNAs bound to ligand ...
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bioRxiv - Biophysics 2020Quote: ... eluate fractions from the Ni2+-NTA column containing the nsp7-8-His6 were concentrated and the buffer exchanged with a PD-10 desalting column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.
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bioRxiv - Molecular Biology 2020Quote: ... The reaction products were resolved on 6–8% (w/v) denaturing polyacrylamide gels and visualized with Typhoon FLA scanner (GE Healthcare). In total ...
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bioRxiv - Microbiology 2021Quote: ... A constant amount of total RNA (between 3.5 and 14 μg) was loaded on 8% urea acrylamide gel in 1X TBE and transferred to a nitrocellulose membrane (GE Healthcare). For detection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Samples of both male and female livers from all the different treatments (N=6-8) were individually used to isolate total RNA via the Illustra RNAspin Mini RNA Isolation kit (GE Healthcare), according to the manufacturer’s standard protocol ...
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bioRxiv - Immunology 2022Quote: ... After equilibration of the IPG strips in reduction buffer and alkylation buffer proteins were separated according to their molecular weight in the second dimension using a gradient gel (8%-15%) in an Ettan DALT II system (GE Healthcare). The gels were then scanned at the different wavelengths using a Typhoon 9400 Laser Scanner (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions were analyzed in a Commasie stained 8% SDS-polyacrylamide gel and gel lanes were scanned using a Typhoon 9400 laser imager (GE Healthcare). Scanned gels were analyzed using ImageQuant TL v2005 software and the two peak fractions were pooled and concentrated to 8.5 mg/ml in 79 μl ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted proteins were analyzed in an 8% SDS-polyacrylamide gel and scanned and quantitated using a Typhoon 9400 laser imager (GE Healthcare).
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bioRxiv - Plant Biology 2021Quote: ... and proteins were separated by SDS/PAGE on 8-10% (w/v polyacrylamide) gels and transferred to PVDF membranes (Semi-Dry-Blot, GE Healthcare). After transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM EDTA (pH 6.8) prior to purification by size exclusion chromatography using a gel filtration column (HiLoad 26/60 Superdex 75 column, GE Healthcare). The sample was concentrated using a 10 kDa molecular weight cut-off centrifugal filter (Merck Millipore) ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: Affinity-purified xtZnT8 was concentrated to 6-8 mg/ml and loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), and was further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Biophysics 2023Quote: ... The NP without MBP containing buffer was diluted to reach 150 mM NaCl concentration by adding 20 mM NaH2PO4 pH 8 and loaded on a heparin column (GE Healthcare) to remove bound bacterial RNA ...
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bioRxiv - Biochemistry 2023Quote: ... a final gel filtration step was performed in GF buffer (30 mM HEPES pH 8, 250 mM NaCl, 10 mM TCEP) using a S200 size exclusion chromatography column (GE Healthcare).
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bioRxiv - Plant Biology 2023Quote: ... gel analysis were pooled and dialyzed against Tris-HCl buffer (30 mM Tris-HCl, pH 8) using PD-10 Desalting Columns (GE Healthcare). The protein concentration was determined by Bradford assays with BSA (bovine serum albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... the unbound fractions were collected and applied to a Sours 15 Q ion exchange sorbent (column V = 8 mL, GE Healthcare) at a rate of 5 mL/min ...