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Citations for GE Life Sciences :
551 - 600 of 2886 citations for 8 HYDROXY 2 METHYL 4 1H PYRAZOL 1 YL QUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... and subjected to size exclusion chromatography at 4°C using a Superdex-200 column (GE Healthcare) and SEC buffer (50 mM Bis-Tris-Propane pH 9.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Equilibrium dissociation constants were obtained at 4°C using a MicroCal VP-ITC system (GE Healthcare). Peptide concentrations between 300-750 μM in the syringe were injected into 25–35 μM pocket domain in the cell ...
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bioRxiv - Biochemistry 2019Quote: ... The purified nanobody was then coupled to CNBr-Activated Sepharose 4 Fast Flow beads (GE Healthcare) to yield the GFP nanotrap ...
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bioRxiv - Plant Biology 2019Quote: ... Ligands were coupled to NHS-activated Sepharose™ 4 Fast Flow beads (GE Healthcare, United Kingdom). Control beads blocked with ethanolamine were prepared in the same way ...
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bioRxiv - Bioengineering 2020Quote: ... 4 °C) and the lysate was loaded onto a StrepTrap HP 5 mL column (GE Healthcare) and purified with the ÄKTA™purifier system (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... D1-4 (CNFY1-704) was purified using a HiLoad 16/600 Superdex 200 pg (GE Healthcare) pre-equilibrated in buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... the media was passed through a Sepharose 4 fast flow gel filtration column (GE Healthcare, 170149001), using the AKTA pure chromatography system (GE Health care) ...
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bioRxiv - Molecular Biology 2019Quote: ... and then analyzed by size-exclusion chromatography at 4°C using a AKTAmicro system (GE Healthcare) mounted with a Superose 6 5/150 increase column (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... samples were digested with 4 µL Sequencing Grade Modified trypsin (0.5 µg/µl; GE Life Sciences) overnight at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... which was underlayed with 4 ml 80% percoll solution (GE Healthcare-Life Sciences, Chicago, IL, USA). Tubes were centrifuged (1600 G ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was purified using Protein G Sepharose 4 Fast flow (GE Healthcare Life Sciences, Pittsburgh, PA) as per manufacturer protocol.
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bioRxiv - Immunology 2020Quote: ... Cell suspension was then pelleted and then re-suspended in 4 mL 40% Percoll (GE Healthcare), layered on 4 mL 70% Percoll and centrifuged at 800xg (RT ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL HisTrap FF column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL StrepTrap HP column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The in-house resin is prepared by coupling N-hydroxysuccinimide (NHS)-Activated Sepharose 4 (GE Healthcare) with 2-(Pyridyldithio ...
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bioRxiv - Pathology 2023Quote: ... 4° C) and purified via anion-exchange chromatography with an AKTA pure chromatography system (GE Healthcare). The sample was passed through an anion-exchange column (HiTrap Q HP ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the supernatant incubated for 1.5 h with IgG-coupled sepharose beads (GE Healthcare). The beads were washed with lysis buffer and proteins eluted by cleavage with TEV protease ...
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bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL; GE Healthcare) for 2hr at RT ...
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bioRxiv - Biophysics 2024Quote: ... for 45 min at 4 °C and loaded on an empty PD-10 column (GE Healthcare). The column was washed with 15 column volumes of wash buffer containing 20 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... The protein extract was precleared by incubation with nProtein A-Sepharose 4 Fast Flow (GE Healthcare) for 30 min and then incubated with 20 uL of rabbit anti-Myc antibody (ab9106 ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
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bioRxiv - Microbiology 2021Quote: ... and applied to column with 2 ml of Ni2+-NTA-agarose (GE Healthcare) equilibrated with wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was loaded on a 2×5ml HisTrap HP column (GE Healthcare) pre-equilibrated with HisBind A ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT on a HiLoad Superdex 75 26/60 column (GE Healthcare). After treatment with Prescission protease overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked in 2% ECL Prime™ Blocking Agent (GE Healthcare, RPN418) in TBST (Tris-Buffered Saline (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... before incubation 2 h with 500 μL CNBr-activated Sepharose beads (GE Healthcare) coupled to monoclonal Rho-1D4 antibody (University of British Columbia ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Fractions were collected in a Whatman 2 ml 96-well plate (GE Healthcare) using a horizontal snaking pattern and were concatenated into 24 final fractions for proteomic analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using 2-D quant kit (GE healthcare, Cat# 80648356), by following manufacturer’ instruction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 mM TCEP) by using a Superdex S75 16/60 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase (HRP)-conjugated IgG F(ab)2 donkey anti-rabbit (GE Healthcare), and HRP-conjugated affinity-purified goat anti-mouse IgG (Jackson).
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bioRxiv - Systems Biology 2020Quote: ... and filtered through a 10 kDa MWCO spin column (Vivaspin 2; GE Healthcare) and quantified by BCA assay (Pierce).
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bioRxiv - Systems Biology 2021Quote: ... agarose-GSH resin and Ni+2-NTA resin were from GE Healthcare (USA); and restriction enzymes from Thermo Fisher (USA) ...
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bioRxiv - Immunology 2020Quote: ... and supplemented to determine culture optima using [200U/mL] IL-2 (GE Healthcare), [155U/mL] IL-7 (Miltenyi Biotech) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM MgCl2) using the PD-10 desalting columns (GE Healthcare, Piscataway, NJ). Quantification of VP2 particles was determined by absorbance at A280 with NanoDrop (NanoDrop2000 ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM NaCl and 2 mM DTT by a desalting column (GE Healthcare), and was further purified by ion exchange chromatography with Resource Q column (GE Healthcare) ...
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bioRxiv - Plant Biology 2023Quote: ... another 2 ml of buffer and 10 μl of PreScission protease (GE Healthcare) were added to the column followed by overnight incubation to cleave off the AVR proteins from the GST resin ...
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bioRxiv - Biochemistry 2022Quote: MCherry-kindlin 2 was bound on a HisTrap FF crude column (GE Healthcare) and eluted with 300 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare), 0.5 μg solids/μl in water was added ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was subsequently incubated with 2 ml Ni-NTA (GE Healthcare) per 1,000 ODs for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... they were ligated with the pGEX-4T-2 plasmid (GE Healthcare Life Sciences) previously digested with the restriction enzymes EcoRI and XhoI ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with 0.5 ml protein-A-sepharose (nProtein A Sepharose 4 Fast flow, GE healthcare #1705280) at 4 °C for 24 hr ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 by gel filtration over Sephadex G25 (GE Healthcare, 4 x HiPrep Desalting 26/10 columns).
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C and injected onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Life Sciences) equilibrated with buffer C (Table S1) ...