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Citations for GE Life Sciences :
301 - 350 of 3299 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
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bioRxiv - Cell Biology 2022Quote: ... incubated in HRP conjugated secondary antibodies detailed in Table 4 for 1 h at room temperature and developed using Pierce SuperSignal Pico Plus (Pierce) or ECL (GE Healthcare) reagent and imaged on ImageQuant.
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bioRxiv - Cancer Biology 2020Quote: ... were cultured according to the ATCC guidelines in 4:1 mixture of Dulbecco’s medium and Medium 1999 supplemented with 10% Hyclone™ FBS (GE Healthcare) and 50μg/ml Gentimicin (Life Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... cleavage was achieved after elution of GbpA from the column by adding 1 unit of PreScission protease enzyme and incubation overnight at 4°C per the instructions of the manufacturer (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: IVTT reaction mixtures (100 µl) were incubated at 4 °C for 1 h with a 50% slurry of PBS/Glutathione-Sepharose 4B (GE Healthcare) with gentle mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were cleared for 30 min at 12,500 × g at 4°C and the supernatant was collected and fractionated on a 1 ml Q-HiTrap column (GE Healthcare) equilibrated in 20 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... to 3 mL for loading onto a 1 × 66 cm gel filtration column comprised of 4 g of degassed Sephadex G-50 beads (GE Healthcare) preequilibrated in buffer A O/N ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Neuroscience 2024Quote: ... The protein bands were detected by incubating the membrane with primary antibodies (see below) overnight at +4°C and horse radish peroxidase-conjugated secondary antibodies (1:5000, NA934 or NA931, GE Healthcare) for 1 h at RT ...
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bioRxiv - Systems Biology 2024Quote: ... overnight at 4°C followed by a 1/10.000 dilution of anti-mouse IgG HRP-linked whole secondary antibody (GE Healthcare NA931), during 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by centrifugation at 50,000⍰×⍰g for 1⍰h at 4⍰°C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample (at a concentration of 3 mg·ml−1) were loaded onto either a Superdex 75 or 200 HR 10/300 GL columns (GE Healthcare, Madison, WI, USA), equilibrated in 25 mM Hepes (pH 7.8) ...
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bioRxiv - Molecular Biology 2023Quote: ... nitrocellulose membranes were blocked in 3 % BSA in TBST and stained with HRP-conjugated streptavidin (GE Healthcare Life Science, Cat# RPN1231vs,1:2500) in blocking solution ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was separated on a Ni Sepharose 6 Fast Flow (GE Healthcare) column using 30 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... hTRPC5 protein was further purified by size exclusion chromatography (Superose-6, GE Healthcare) in buffer containing TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... which was further purified using a Superose 6 10/300 column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 ml of Ni2+-beads slurry (Ni-sepharose 6 Fast Flow, GE Healthcare) were washed in washing buffer (20 mM TrisHCl pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... and then loaded onto a Superose 6 increase column (GE Healthcare, Chicago, IL) on an NGC system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified with Ni-sepharose fast flow 6 resin (GE Healthcare, Boston, USA) by the elution with 250 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the size-exclusion chromatography (Superose 6 10/300 GL, GE Healthcare) in buffer containing 20 mM MOPS-Na ...
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bioRxiv - Molecular Biology 2021Quote: ... and was injected into a Superose 6 Increase 10/300 column (GE Healthcare) in buffer H200 (20 mM Hepes ...
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bioRxiv - Biochemistry 2020Quote: ... The clear supernatant was incubated with Ni-Sepharose 6 Fast Flow (GE Healthcare) to capture the His-tagged protein ...
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bioRxiv - Bioengineering 2021Quote: ... The column was a Superose 6 Increase 10/300 GL from GE healthcare. Data was analyzed using Astra 6.0 software ...
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bioRxiv - Biophysics 2021Quote: ... The eluate was concentrated and loaded onto Superose 6 increase column (GE Healthcare) running with TBS supplemented with 0.1% digitonin ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were injected through a Superose 6 10/300 GL column (GE Healthcare) equilibrated in 20 mm HEPES ...
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bioRxiv - Microbiology 2020Quote: ... HDL was purified by size fractionation on a Superose 6 column (GE Healthcare) equilibrated with TBS ...
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bioRxiv - Plant Biology 2020Quote: ... and then immobilized on Ni Sepharose 6 Fast Flow beads (GE Healthcare, 10249123) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... and then immobilized on Ni Sepharose 6 Fast Flow beads (GE Healthcare, 10249123). The beads were subsequently washed and eluted by buffers as described above.
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bioRxiv - Biophysics 2021Quote: ... Nanodiscs were then purified on a Superose 6 10/300 GL (GE Healthcare) equilibrated with 0.2 M ammonium acetate ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 and DPP4 were further purified using a Superose 6 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... 500μL total protein was incubated with 20μL IgG-Sepharose 6 resin (GE healthcare) for 2 hrs at 4°C with rotation ...
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bioRxiv - Biophysics 2020Quote: ... and further purified by Superose 6 Increase 10/300 GL column (GE Healthcare) in 25 mM Mops (pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... Prior to crystallization the protein was loaded onto a Superose 6 (GE Healthcare) size-exclusion column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2022Quote: ... gel filtration was performed using a Superose 6 10/300GL column (GE Healthcare) fitted to an AKTA explorer system (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... and gel filtered through a Superose 6 10/300 GL column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Immunology 2022Quote: ... proteins were separated on a Superose 6 Increase 10/300 column (GE Healthcare) in 1x PBS (pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... and run through a size exclusion column (Superose 6 10/300, GE Healthcare). Fractions containing purified monomeric Tau were concentrated as before and buffer exchanged to PBS ...
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bioRxiv - Cell Biology 2022Quote: ... analytical size exclusion chromatography was performed in a Superose 6 column (GE Healthcare) which was fitted to the Thermo Scientific Vanquish HPLC system ...
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bioRxiv - Cell Biology 2022Quote: ... The recombinant proteins were purified by Ni+ sepharose 6 fast flow (GE Healthcare), glutathione Sepharose 4B (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... and purified with superpose 6 increase (GE Healthcare Life Sciences, Pittsburgh, PA, USA) size exclusion column.