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Citations for GE Life Sciences :
251 - 300 of 3299 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and further purification by RESOURCE Q 6 mL column chromatography (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2020Quote: ... Deglycosylated protein was then purified using a Superose 6 column (GE healthcare) equilibrated with buffer A ...
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bioRxiv - Biophysics 2020Quote: ... and 150 mM NaCl (Superose 6 Increase 10/300 GL, GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... and resuspended in 6 ml of 38% isotonic Percoll® (GE Healthcare) before a 25 min centrifugation at 800 G without neither acceleration nor brake ...
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bioRxiv - Molecular Biology 2020Quote: ... filter and run on a Superose™ 6 Increase column (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... and applied to a Superose 6 Increase 10/30 column (GE Healthcare), equilibrated in gel filtration buffer ...
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bioRxiv - Biophysics 2023Quote: ... and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer plus 0.03% digitonin ...
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bioRxiv - Biophysics 2023Quote: ... before gel filtration using a Superose 6 10/300 column (GE Healthcare) equilibrated with GF buffer (20 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by Ni2+-Sepharose 6 Fast Flow (GE healthcare) affinity chromatography with the washing buffer (50mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Clear lysates were incubated with IgG Sepharose 6 Fast-Flow (GE Healthcare) for four hours at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... and size exclusion column (Superose 6 Increase 10/300 GL, GE Healthcare) in buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were bound to IgG Sepharose 6 Fast-Flow (GE Healthcare) via the ZZ tag on the heavy chain ...
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bioRxiv - Molecular Biology 2023Quote: ... A Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) was pre-equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded onto Superose 6 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with buffer including 20 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified on a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer GF (10 mM HEPES–NaOH pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... or a Superose 6 prep grade XK 16/600 column (GE Healthcare). Columns were equilibrated in Ndc80 buffer (50 mM Hepes ...
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bioRxiv - Biochemistry 2022Quote: ... The 6% acrylamide gels were scanned on a Typhoon scanner (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... and loaded onto a Superose 6 increase column (GE Healthcare, Chicago, IL) on an NGC system (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The 6% acrylamide gels were scanned on a Typhoon scanner (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in TBS with 0.01% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... and loaded into a Superose 6 Increase 10/300 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Biophysics 2021Quote: ... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of the resulting supernatant was incubated for 1 hour at 4°C on constant agitation of 1,000 rpm with 100 µL glutathione sepharose (GS) resin (GE Healthcare), pre-equilibrated in buffer F ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant after centrifugation at 17,000 g at 4 °C for 1 hr was incubated with Glutathione Sepharose 4B (GE healthcare) at 4 °C for 3 hrs ...
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bioRxiv - Biochemistry 2021Quote: ... the purified OxlT was mixed with purified D5901-A08-Fab at a 1:1.3 molar ratio at 4 °C overnight and applied to a HiLoad 16/60 Superdex200 pg column (GE healthcare) using buffer D (20 mM MES-KOH ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was then centrifuged for 1 h at 16,000 g at 4 °C and the supernatant was added to 1 mL of glutathione beads (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Swarmer cells were isolated from the culture by centrifugation (20 mins at 11,000 rpm, 4°C) after mixture with 1 volume of Percoll (GE Healthcare). The synchronized swarmer cells were pipetted onto an agarose (2% ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded at low speed (0.2 ml/min) at 4°C onto a HisTrap-HP 1 mL affinity column (GE Healthcare) equilibrated with buffer A (20 mM NaPi pH 7.7 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to Amersham™ Protran® Western blotting membranes, nitrocellulose (pore size 0.2 μm, roll W × L 300 mm × 4 m, pkg of 1 ea) (GE healthcare) overnight 4 °C at 15 V ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation (15’000 xg, 4 °C, 30 minutes) and supernatants were incubated with 1 ml Glutathione Sepharose (GE Healthcare) for 2 hours at 4 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Samples were homogenised by bead beating at 4°C in 500 μl Tris-HCl lysis buffer (100 mM, pH 6.8) containing 1% Protease Inhibitor Mix (GE Healthcare). A full and detailed description of the protocol for 2-dimensional gel electrophoresis (2-DE ...
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bioRxiv - Immunology 2023Quote: ... [3] A HisTrap FF column (Cytiva, GE Healthcare) was used in the Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3–5 times and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rabbit antibody (GE Healthcare, UK) for one hour ...
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bioRxiv - Microbiology 2023Quote: ... equivalent to ∼1-3×108 cells per sample) were collected by vacuum filtration onto glass microfiber filters (grade GF/A, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... and the cleaved DRH-3 CTD and DRH-3 NTD were purified using a Superdex 75 HiLoad 16/600 size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 5-15 mg ml−1 in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Cancer Biology 2022Quote: Custom siRNAs targeting 5’-CACCAUGAGUGGCAGUCAG-3’ 33 for FRA1 and 5′-GUUCACUGCUGGCCUAUAA-3’ 34 for FLI1 were ordered from GE Healthcare Dharmacon ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation (15000 rpm for 1 h at 4 °C) and incubated with glutathione Sepharose 4B (GE Healthcare) for 4 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and chromatin was released from the resin with 20 µl of ChIP buffer 1 supplemented with 100 µg/ml BSA and 4 µg HRV3C incubated (GE Healthcare) on ice for 60 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were then incubated with SuperSignal West Dura or ProSignal Pico Spray chemiluminescence substrate for 1-4 minutes at room temperature and imaged using an Amersham Imager 600 (GE Healthcare) or ChemiDoc (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then cleared by centrifugation (18000 g, 4 °C, 1 hour) and purified using a HisTrap HP 5 ml column (GE Healthcare) and a 10-100 mM imidazole linear gradient followed by elution with 250 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 12000 g at 4 °C for 1 h and loaded onto a HisTrap HP column (GE Healthcare) equilibrated with 98% buffer A/2% buffer B (20 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...