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Citations for GE Life Sciences :
3401 - 3450 of 3622 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... Imidazole was added to the supernatant to final concentration of 10mM loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare) equilibrated in NiNTA A buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL of purified proteins at 5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Biophysics 2023Quote: ... The cell lysates were filtered through a 0.45 μm syringe filter and purified by affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare Life Sciences) with 5 CV of wash buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... The cell lysates were filtered through a 0.45 μm syringe filter and purified by affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare Life Sciences) with 5 CV of wash buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Purity and molecular weights were analyzed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analytical size exclusion chromatography (SEC) using a Superdex™ 75 5/150 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: Human packed RBC were spin washed (1800 rpm; 5 minutes) three times with chilled 1X PBS and resuspended in RPMI (HyClone, GE Healthcare, USA) at 2% haematocrit ...
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Improved Activity and Kinetics of Endoglucanase Biofuel Enzyme with Addition of an AzoTAB SurfactantbioRxiv - Bioengineering 2023Quote: ... The protein solution was filtered and applied to a 5 mL HiTrap Q HP anion exchange column (GE Healthcare, Cat. no. 17115301) and later washed with 30× column volume with the protein buffer ...
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bioRxiv - Biochemistry 2023Quote: The supernatant containing the target protein was then loaded on a 5 mL Ni-NTA column followed by a size exclusion column (Superdex 75, GE Healthcare, USA). YsiA is then eluted stepwise with 30 mM ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing Fibril Δ(273-512)-His6 was loaded into a pre-packed 5 mL Ni-NTA column (HisTrap; GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... beads were suspended in 0.22 mm filtered PBS with 5 ml (10 units) of PreScission protease (catalog # 27084301; GE healthcare Life Sciences) and incubated overnight at 4°C on a rotospin test tube rotator for on-column PreScission protease cleavage ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting lysate was filtered through a 0.2 μm nylon filter and loaded onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences) that had been equilibrated in Buffer A ...
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bioRxiv - Microbiology 2024Quote: ... The crude cell extract was cleared by centrifugation for 30 min at 16,000 ×rpm (4 °C) and loaded onto a 5 ml HisTrap™ HP affinity column (GE Healthcare, USA) equilibrated with buffer B ...
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bioRxiv - Genomics 2024Quote: ... Peripheral blood mononuclear cells (PMBCs) were also isolated from 5 ml of blood using the standard Percoll ® (GE Healthcare, Chicago, USA) centrifugation method ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved protein was applied to a Source 15 Q anion exchange column and eluted with a gradient of 200 mM-300 mM NaCl in 20 mM HEPES (pH 7.0) and 2 mM DTT followed by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was collected and incubated with 2 column volume (for 20 mL supernatant) of Gluthatione Sepharose 4B (GE Healthcare) for 1h30 on a rotating wheel ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... was expressed in E.Coli Rosetta 2 (DE3) and purified by affinity chromatography over a Sepharose 4B GSH affinity column (GE healthcare) followed by gel filtration over a Hiload Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Ycf1p NBD1 and NBD1-R were exchanged into the fluorescence buffer (20 mM Na3PO4, 2% glycerol, 150 mM NaCl, pH 7.3) by size exclusion chromatography (Superdex75, GE Healthcare). MgATP-bound and apo NBD1 and NBD1-R were generated by adding 2 mM MgATP or 2 mM EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved protein was applied to a Source 15 Q anion exchange column and eluted with a gradient of 150 mMà350 mM NaCl in 20 mM Immidazole (pH 8.0) and 2 mM DTT followed by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... Nbs and their mixture (1.6 nmol of SARS-CoV-2 RBD mixed with 1.6 nmol of Nbs) were run over a Superdex 75 column (GE healthcare) at a flow rate of 0.5 mL/min with AKTA pure.
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids encoding for GST fusion proteins of Cobl-like1-411 were generated by subcloning into pGEX-4T-2 (GE Healthcare). A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Biochemistry 2020Quote: ... MRPP1 and 2 formed a complex of ~150 kDa that was further purified by gel-filtration (Superdex 200, GE Healthcare). His6-Δ95 MRPP3 cDNA lacking the N-terminal disordered region was cloned into pMCSG7 vector and expressed and purified ...
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bioRxiv - Immunology 2020Quote: ... 7.5×104 congenically distinct cells of each sorted memory population were adoptively transferred and tumor-bearing mice were subsequently immunized with 10 μg of GP33-41 and 2 μg of poly(I:C) (GE Healthcare) s.c ...
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bioRxiv - Genetics 2020Quote: ... 2-4 mg protein extract was added to 50 μl of Glutathione Sepharose beads (Cat. no. 17527901, GE healthcare Ltd.) equilibrated with 1XPBS and incubated for 10-14 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: All samples were prepared in a total volume of 40 μL in SEC buffer A supplemented with 2 mM DTT and analysed on a Superdex 75 3.2/300 Increase column (GE Healthcare). For the TTP-DCP2 interaction ...
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bioRxiv - Immunology 2021Quote: ... the protein was diluted with 2-fold volume of 20 mM HEPES with pH 7.0 and loaded on HiTrap SP HP column (GE Healthcare) equilibrated with 100 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... fragments or Fabs 2/1.12 or 2/6.14 were either used as analytes or amine immobilized on a CM5 chip in a Biacore 3000 (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: HEK293A cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 2 mM L-glutamine (GE Healthcare Life Sciences), 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... Eluted protein was concentrated down to 2 mL for size exclusion chromatography with a Superose6 16/600 column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Cell Biology 2022Quote: Human isoform of amphiphysin 2/BIN1 including exon 11 was expressed in Rosetta 2 bacteria and purified by affinity chromatography using glutathione Sepharose 4B beads according to the manufacturer’s instructions (GE Healthcare) in 50 mM Tris at pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... the complex was further purified by anion exchange chromatography in presence of 2% CHAPS using the AKTA system (GE Healthcare), flash frozen and stored at −80 °C until use.
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl.) supplemented with 2 mM CaCl2 and loaded onto a Superose6 Increase 10/300 GL column (GE Healthcare) integrated on a high-performance liquid chromatography (HPLC ...
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bioRxiv - Plant Biology 2023Quote: ... first-strand cDNA was synthesized from 2 µg of total RNA using Ready-to-Go RT-PCR beads (GE healthcare) and an oligo(dT ...
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bioRxiv - Evolutionary Biology 2023Quote: ... algae were grown for two weeks and harvested using a vacuum filtration with Whatman #2 paper (GE Healthcare 47 mm), washed with distilled water (three times) ...
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bioRxiv - Microbiology 2023Quote: ... 200 mM NaCl and 2 mM TCEP using a Superdex-200 10/300 GL size-exclusion column from GE Healthcare. All protein concentrations were diluted to 1.7 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... the beads were boiled with 2 × Laemmli’s sample buffer and subjected to immunoblotting using an Amersham ECL Prime Western Blotting Detection System (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... 50-100 μL of the purified protein samples (concentration ranging 2-15mg/ml) were filtered (with 0.1 μm pore size) and loaded onto a Superdex200 10/300GL column (GE Healthcare) preequilibrated with SEC buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... the combined peptide containing solutions were passed over pre-equilibrated (50 µl 0.5 % formic acid ) home-made 2-disc Glass microfiber StageTip (disc material: GE Healthcare; pore size ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of protein was immobilized on a nitrocellulose membrane (Amersham) by filtration using a slot blot apparatus (GE Healthcare). The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... Elution buffer was exchanged to standard Tris protein buffer (100 mM Tris and 150 mM NaCl at pH 7.5) with NAP-5 columns (GE Healthcare, Chicago, IL, USA) and samples were concentrated as before.
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and purified using immobilized metal affinity chromatography via a HisTrapTM HP 5 mL (GE Healthcare, Chicago, IL, USA) and an AKTA FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... sfGFP-ACE2 was purified by an ion exchange column (HiTrap 5 ml Q FF anion exchange chromatography column; GE Healthcare, U. S. A.) eluted by a linear salt gradient from 10 to 1000 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 5 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...