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Citations for GE Life Sciences :
3201 - 3250 of 4264 citations for Recombinant Human CFHR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to 0.45 µm pore-size nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked at room temperature for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2024Quote: ... Refolded HLA-G2 protein was purified by size exclusion chromatography using HiLoad 26/60 Superdex75 pg column (GE Healthcare) equilibrated with 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml of pooled mouse sera was then used to purify IgG using Protein A Sepharose resin (GE Healthcare) and digested for using papain-agarose resin (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... 500 µL sample was injected and run with a flow rate of 0.5 mL/min on an ÄKTA Pure protein purification system (GE Healthcare). Control samples containing ThT alone at the lowest (0.4 M urea ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were finally purified using size exclusion chromatography using a Superdex 200 column (10/300 GL, GE Healthcare, Germany) equilibrated with PBS buffer ...
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bioRxiv - Cell Biology 2020Quote: Fresh embryo protein extracts (4 mg in 2.5 ml RIPA buffer) was passed through PD-10 desalting column (GE Healthcare) to remove excess free biotin using the gravity protocol according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was concentrated using Amicon-3.5K filters and subjected to size exclusion chromatography on a Superdex 200 column (GE Healthcare). The concentration of eluted proteins was measured at 280 nm using a Nanodrop spectrophotometer and the proteins were verified by Coomassie Blue staining and western blotting ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was precleaned with 40 μL of 50% slurry of protein A Sepharose beads (GE Healthcare, Chicago, IL, USA) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The fractions containing the protein were concentrated and gel filtered on a Superdex 75 increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected by staining with Coomassie Brilliant Blue R250 or by transferring to a PVDF membrane (GE Life Sciences), and staining with Ponceau S dye or detection by immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions with >95% pure protein were pooled and re-purified from HiTrap Heparin HP affinity columns (GE Healthcare Life sciences) using a linear gradient of 0 – 300 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Fc mutations to prevent dimerization were introduced into the Fc sequence.61 Proteins were purified using MabSelect resin (GE Healthcare). Proteins were biotinylated using BirA enzyme (BPS Biosciences ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then loaded onto a transport buffer equilibrated Superdex 75 gel filtration column (GE Healthcare, #28-9893-33) and fractions containing the protein of interest were pooled ...
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bioRxiv - Molecular Biology 2020Quote: ... For each IP 100µL chromatin was pre-cleared with BSA blocked protein-G sepharose beads (GE Healthcare, Chicago, IL, USA) and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449 ...
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bioRxiv - Molecular Biology 2021Quote: Released protein was further concentrated to 0.5mL and then loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... Exposure of the resultant protein bands was performed with an ImageQuant LAS 4000 Luminescent Image Analyzer (GE Healthcare; Chicago, IL).
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bioRxiv - Microbiology 2022Quote: ... Cells were separated from supernatant by centrifugation and supernatant containing the secreted proteins was loaded onto a NiFF (GE Healthcare) column as described above.
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bioRxiv - Microbiology 2019Quote: ... Strep-KaiC36803 and Strep-KaiC7942 proteins were applied on a Sephacryl S300 HR HiPrep 16/60 Sephacryl column (GE Healthcare) and separated in 20 or 50 mM Tris/HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were cleared by centrifugation (20,000 rpm, 60 min) and proteins were purified from the supernatant using 7mL of Ni-NTA resin (GE Healthcare). The protein was eluted from the column with nickel-binding buffer containing 500mM imidazole.
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bioRxiv - Biochemistry 2019Quote: ... to 3 ml and the protein further purified by size exclusion chromatography (Hiload 16/600 Superdex 200 pg, GE healthcare) equilibrated with SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then run over a size exclusion column (GE Healthcare HiPrep™ 26/60 Sephacryl S-300 HR) in sizing buffer (50mM Tris-HCl pH=8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... Protein-containing fractions were concentrated and further purified on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... Fluorescence profiles and peak volumes of labeled proteins were obtained using ImageQuant TL v2005 (GE Healthcare Life Sciences, Piscataway, NJ). For calculation of AA74-1 IC50 values (Fig ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was purified by size exclusion chromatography over a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... the protein of interest was enriched via metal ion affinity chromatography (Ni-NTA) resin (IMAC, 5ml HiTrap Chelating, GE Healthcare). After 10 column washes with (20 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: A volume of 25-30 µL serum was spotted onto a Whatman® Protein Saver™ 903 Card (GE Healthcare), air-dried at room temperature for approximately 2 hours and stored at 4 °C until further use ...
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bioRxiv - Cell Biology 2019Quote: ... Processed protein was concentrated and further purified on a HiLoad Superdex 200 16/60 size exclusion chromatography column (GE Healthcare) equilibrated in 20 mM Tris HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysates were run on an 8% SDS-PAGE gel and the protein was then transblotted onto a 0.2 µm nitrocellulose membrane (GE Healthcare), followed by blocking for 3 hours in KPL block (SeraCare ...
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bioRxiv - Microbiology 2019Quote: ... 2.5 ml of fractions containing the highest protein content were pooled and the imidazole was removed by running the 2.5 ml protein suspension on PD-10 size-exclusion columns (GE Healthcare) followed by cold 3.5 ml of Buffer A to elute proteins ...
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Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... Refolded protein was concentrated (Vivaspin or Amicon 10 kDa) and further purified on Superdex S75 or S200 columns (GE Healthcare) in SEC buffer as indicated in Table S3 ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 μg proteins were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). To visualize MLKL oligomers ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were clarified by centrifugation at 12,000 × g for 15 min and GST-Rab proteins were applied at saturating levels to glutathione Sepharose beads (GE Healthcare) for 30 min at 4°C and then washed extensively to remove unbound material ...
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bioRxiv - Immunology 2021Quote: ... TCR and pMHC proteins were dialyzed and further purified via anion exchange chromatography (HiTrap Q HP; Mono Q; GE Healthcare) and size-exclusion (Superdex 200 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein signal was detected by Clarity™ western ECL substrate and visualized by LAS680 imager (GE Life Sciences, Pittsburgh, USA). Images were analyzed by ImageJ (NIH ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was separated from free D3_Sof protein by size-exclusion chromatography using “Superdex 75 10/300 GL” column (GE Healthcare) at 0.4 ml/min flow rate using running buffer containing 25 mM Tris-HCl (pH 7.6 at 25 ° C) ...
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bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2021Quote: ... and protein further purified by size-exclusion chromatography using a HiLoad 16/600 Superdex 75 pg column (GE Life Sciences), 1.0 mL/min in Na2HPO4 pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... the bound fusion protein was eluted with 20– 200 mM imidazole gradient using a FPLC purifier system (ÄKTA; GE Healthcare). Peak fractions were pooled and digested with SENP2 protease (at final 0.01 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was further purified over a gel filtration column (HiPrep 16/60 Sephacryl S-100 High Resolution, GE Healthcare) equilibrated with AUC buffer (50 mM NaH2PO4 pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... 100-400 mM NaCl (depending on the protein) with either PD-10 or HiPrep 26/10 desalting columns (GE Healthcare). SGTA ...
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bioRxiv - Biochemistry 2020Quote: Proteins were transferred from the SDS-PAGE gel to a 0.2 μm Amersham Protran Nitrocellulose blotting membrane (GE life sciences) using a BioRad Trans-blot cell gel tank filled with transfer buffer (192 mM glycine ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Biochemistry 2020Quote: ... The clarified supernatant containing the protein was loaded on a 5 mL HiTrap Ni-chelating column (GE Healthcare, Piscataway, NJ), washed ...
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bioRxiv - Biochemistry 2021Quote: ... The excess Cu(II) was eliminated by passing the protein through a PD MidiTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl.
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bioRxiv - Biophysics 2020Quote: ... Supernant containing soluble proteins was filtered using a 0.45 μm device and applied to a HisTrap HP affinity column (GE Healthcare) equilibrated in buffer A (40 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... The complex protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...